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The map label for this gene is ntaB [H]

Identifier: 110679571

GI number: 110679571

Start: 2207896

End: 2208405

Strand: Direct

Name: ntaB [H]

Synonym: RD1_2312

Alternate gene names: 110679571

Gene position: 2207896-2208405 (Clockwise)

Preceding gene: 110679570

Following gene: 110679572

Centisome position: 53.42

GC content: 59.02

Gene sequence:

>510_bases
ATGCTGCACTTCGAGCCCACACAGGACAACCACCGCATGTTTCGCGATGCGTTGGGACGGTTTGCATCAGGTGTGACGAT
CGTGACGGCCAATACGCCGCAGGGGCCGGTCGGCATGACGGCCAATTCCTTTACCAGCGTTTCGATGGAGCCGCCGCTGG
TTCTATGGTGCCCGGCGAAATCGTCCAGCCGCTACGGGCTGTTTGCGCAGGCCACTGATTTCGCGATCCATGTGCTCGGG
GCAGATCAAAAGGCACTTGCTCTTGCCTTTGCGCGCTCGGGCACGGCATTCGAGGCGATTGATGCCGCCCTCAGCGACCG
GGACGTCCCGATTTTTGATGCTTGTCTGGCGCGGTTTGAGTGTGAAACCTACGCGCTGCACGATGCGGGTGATCATTCGA
TCATGGTCGGCATGGTGCGCCACGCGGCGTTCCGGCATGGCGCGCCGCTGATTTTTTCGCAAGGTGACTTCGGACAATTT
GACAAAGAGGTTGAAAAGGCGAACGTATAA

Upstream 100 bases:

>100_bases
CGAAGGTTGAGATGGACCGCCAGACGTGGTTTGAGCTGTTTGAAGCCGCAAACGGGCATGAGGTGCCTTGATGCCGGATG
GAACCACCAAAGGGACGGCG

Downstream 100 bases:

>100_bases
AACGGCGGGGAAACCCGCGCGCCGCCGGCAGAATGTCGGTTGCAGGGAGGAGGCCCATTCATGGGGGCACTGCTGCTGGT
GCTTGCACCGCTTCGGGTGA

Product: nitrilotriacetate monooxygenase component B

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MLHFEPTQDNHRMFRDALGRFASGVTIVTANTPQGPVGMTANSFTSVSMEPPLVLWCPAKSSSRYGLFAQATDFAIHVLG
ADQKALALAFARSGTAFEAIDAALSDRDVPIFDACLARFECETYALHDAGDHSIMVGMVRHAAFRHGAPLIFSQGDFGQF
DKEVEKANV

Sequences:

>Translated_169_residues
MLHFEPTQDNHRMFRDALGRFASGVTIVTANTPQGPVGMTANSFTSVSMEPPLVLWCPAKSSSRYGLFAQATDFAIHVLG
ADQKALALAFARSGTAFEAIDAALSDRDVPIFDACLARFECETYALHDAGDHSIMVGMVRHAAFRHGAPLIFSQGDFGQF
DKEVEKANV
>Mature_169_residues
MLHFEPTQDNHRMFRDALGRFASGVTIVTANTPQGPVGMTANSFTSVSMEPPLVLWCPAKSSSRYGLFAQATDFAIHVLG
ADQKALALAFARSGTAFEAIDAALSDRDVPIFDACLARFECETYALHDAGDHSIMVGMVRHAAFRHGAPLIFSQGDFGQF
DKEVEKANV

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18310; Mature: 18310

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFEPTQDNHRMFRDALGRFASGVTIVTANTPQGPVGMTANSFTSVSMEPPLVLWCPAK
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCC
SSSRYGLFAQATDFAIHVLGADQKALALAFARSGTAFEAIDAALSDRDVPIFDACLARFE
CCCCCEEEEECCCEEEEEECCCCHHEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHC
CETYALHDAGDHSIMVGMVRHAAFRHGAPLIFSQGDFGQFDKEVEKANV
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLHFEPTQDNHRMFRDALGRFASGVTIVTANTPQGPVGMTANSFTSVSMEPPLVLWCPAK
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCC
SSSRYGLFAQATDFAIHVLGADQKALALAFARSGTAFEAIDAALSDRDVPIFDACLARFE
CCCCCEEEEECCCEEEEEECCCCHHEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHC
CETYALHDAGDHSIMVGMVRHAAFRHGAPLIFSQGDFGQFDKEVEKANV
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]