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The map label for this gene is metH [H]

Identifier: 110679525

GI number: 110679525

Start: 2161087

End: 2162109

Strand: Reverse

Name: metH [H]

Synonym: RD1_2260

Alternate gene names: 110679525

Gene position: 2162109-2161087 (Counterclockwise)

Preceding gene: 110679526

Following gene: 110679524

Centisome position: 52.31

GC content: 63.25

Gene sequence:

>1023_bases
ATGCCCAACCCTTTCAGCGATCTTCTTGAAACGCGCGGCGTATTGTTGGCCGATGGCGCGACCGGCACGAATCTCTTCAA
CATGGGATTGATGTCCGGCGATGCACCCGAATTGTGGAACACGCAGGAGCCGCAAAAGATCAAGGCGCTGTACAAAGGCG
CGGTCGATGCGGGCAGTGATCTGTTTCTGACCAATTCCTTCGGAGCCAACGCCGCGCGCCTGAAACTGCACAGCGCCGAG
AAACGCGTTCATGAGTTGAGCCGCGTCGCTGCCGAGATCGGACGCGAGGTCGCGGATACCGCCGGACGGGCGGTGATTGT
CGCAGGCTCCGTCGGGCCGACCGGTGAGATCATGGAGCCTGTTGGCACGCTCAGCCATGCGCTGGCGGTTGAGATGTTTC
ACGAAACCGCAGACGGTCTGAAAGCGGGCGGTGCGGATATCGGATGGCTCGAAACGATCAGCGCACCTGAGGAATACCGC
GCCGCAGCCGAGGGCTTCGCGCTCGCCGGGCTGGATTGGTGCGGCACGATGAGCTTTGACACCGCCGGGCGCACCATGAT
GGGGCTGACCAGCGCCGATATGGTCAAAATGGTCGATGATCTGGGTGATCTTGCGCCGCTGGCTTTTGGGGCGAATTGCG
GCACCGGCGCGTCTGACCTGTTGCGCACGATCCTCGGGTTTGCCGCGCAGGGCGCCGAACGCCCGATCATTGCCAAGGGC
AACGCCGGCATTCCCAAATACGTGGACGGTCACATTCATTACGACGGCACCCCCGAACTGATGGCGGATTACGCCGTGAT
GGCGCGGGCGAGCGGTGCGCGGATCATTGGCGGATGTTGTGGCACCATGCCGGAGCATCTGCGCCATATGCGCGAGGCGC
TGGATACGCGCAGGATGGACCACCCCCCGGCGTTGGAGGAAATCGTGGACCGGCTCGGTGCCTTTTCCTCCGTTGCGGAC
GGGACCGACAAGGAAAACACGCCGGCGCGCCGCGCAACGCGAAGAAGCAGACGCGCCGGCTAA

Upstream 100 bases:

>100_bases
CACCGGGGTCTTCGTCATTTCATGTAAATGATGAGAATTATGAATTTGCGACGGCACGCAACCTGTGACATGACGCACGC
AAGGACAGCGGAGCGTGATC

Downstream 100 bases:

>100_bases
TTCCCGTATTCCGACCCTATTGCCGCCGCACCCGATGCATGCACCGCGTGACACTTTCGCCCGCGCGCGTCGCACTCCGC
TTTCACCGTGTCGCAATCCG

Product: methionine synthase I

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPNPFSDLLETRGVLLADGATGTNLFNMGLMSGDAPELWNTQEPQKIKALYKGAVDAGSDLFLTNSFGANAARLKLHSAE
KRVHELSRVAAEIGREVADTAGRAVIVAGSVGPTGEIMEPVGTLSHALAVEMFHETADGLKAGGADIGWLETISAPEEYR
AAAEGFALAGLDWCGTMSFDTAGRTMMGLTSADMVKMVDDLGDLAPLAFGANCGTGASDLLRTILGFAAQGAERPIIAKG
NAGIPKYVDGHIHYDGTPELMADYAVMARASGARIIGGCCGTMPEHLRHMREALDTRRMDHPPALEEIVDRLGAFSSVAD
GTDKENTPARRATRRSRRAG

Sequences:

>Translated_340_residues
MPNPFSDLLETRGVLLADGATGTNLFNMGLMSGDAPELWNTQEPQKIKALYKGAVDAGSDLFLTNSFGANAARLKLHSAE
KRVHELSRVAAEIGREVADTAGRAVIVAGSVGPTGEIMEPVGTLSHALAVEMFHETADGLKAGGADIGWLETISAPEEYR
AAAEGFALAGLDWCGTMSFDTAGRTMMGLTSADMVKMVDDLGDLAPLAFGANCGTGASDLLRTILGFAAQGAERPIIAKG
NAGIPKYVDGHIHYDGTPELMADYAVMARASGARIIGGCCGTMPEHLRHMREALDTRRMDHPPALEEIVDRLGAFSSVAD
GTDKENTPARRATRRSRRAG
>Mature_339_residues
PNPFSDLLETRGVLLADGATGTNLFNMGLMSGDAPELWNTQEPQKIKALYKGAVDAGSDLFLTNSFGANAARLKLHSAEK
RVHELSRVAAEIGREVADTAGRAVIVAGSVGPTGEIMEPVGTLSHALAVEMFHETADGLKAGGADIGWLETISAPEEYRA
AAEGFALAGLDWCGTMSFDTAGRTMMGLTSADMVKMVDDLGDLAPLAFGANCGTGASDLLRTILGFAAQGAERPIIAKGN
AGIPKYVDGHIHYDGTPELMADYAVMARASGARIIGGCCGTMPEHLRHMREALDTRRMDHPPALEEIVDRLGAFSSVADG
TDKENTPARRATRRSRRAG

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=289, Percent_Identity=28.3737024221453, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1790450, Length=319, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535405, Length=319, Percent_Identity=28.8401253918495, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 35841; Mature: 35710

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPFSDLLETRGVLLADGATGTNLFNMGLMSGDAPELWNTQEPQKIKALYKGAVDAGSD
CCCHHHHHHHHCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
LFLTNSFGANAARLKLHSAEKRVHELSRVAAEIGREVADTAGRAVIVAGSVGPTGEIMEP
EEEECCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VGTLSHALAVEMFHETADGLKAGGADIGWLETISAPEEYRAAAEGFALAGLDWCGTMSFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCEEECCHHHCCCCCC
TAGRTMMGLTSADMVKMVDDLGDLAPLAFGANCGTGASDLLRTILGFAAQGAERPIIAKG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECC
NAGIPKYVDGHIHYDGTPELMADYAVMARASGARIIGGCCGTMPEHLRHMREALDTRRMD
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
HPPALEEIVDRLGAFSSVADGTDKENTPARRATRRSRRAG
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PNPFSDLLETRGVLLADGATGTNLFNMGLMSGDAPELWNTQEPQKIKALYKGAVDAGSD
CCHHHHHHHHCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
LFLTNSFGANAARLKLHSAEKRVHELSRVAAEIGREVADTAGRAVIVAGSVGPTGEIMEP
EEEECCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VGTLSHALAVEMFHETADGLKAGGADIGWLETISAPEEYRAAAEGFALAGLDWCGTMSFD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCEEECCHHHCCCCCC
TAGRTMMGLTSADMVKMVDDLGDLAPLAFGANCGTGASDLLRTILGFAAQGAERPIIAKG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECC
NAGIPKYVDGHIHYDGTPELMADYAVMARASGARIIGGCCGTMPEHLRHMREALDTRRMD
CCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
HPPALEEIVDRLGAFSSVADGTDKENTPARRATRRSRRAG
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]