Click here to switch to the map view.

The map label for this gene is hsdS [H]

Identifier: 110679503

GI number: 110679503

Start: 2136203

End: 2137342

Strand: Direct

Name: hsdS [H]

Synonym: RD1_2234

Alternate gene names: 110679503

Gene position: 2136203-2137342 (Clockwise)

Preceding gene: 110679502

Following gene: 110679504

Centisome position: 51.69

GC content: 50.44

Gene sequence:

>1140_bases
GTGAAAGCAGGTTGGGAGGTTAAGCCGCTTGGCGAAGTTGCAAAATTGCACTATGGAAAAGCCCTTGCAGAATCTGAGCG
TAGTCCAAACGGCACTGTGCCAGTGTACGGTGCAAATGGTGTTCTGGGTTGGTCCGACCATACACTTACAGAAGGCCCCT
CGCTCATCGTTGGCCGTAAAGGTTCCGCAGGAGAGGTCAATCGTGTAGACGGCCCGTTTTGGCCCAGCGATGTAACGTAT
TACACCGAGCACGACCCAAATCGTCTGGACTTCGACTATTTCCACTATGGTCTAATGACTCTAAATCTCCCGTCTCTGGC
AAAAGGTGTGAAGCCCGGAATAAACCGTAATGACGTGTACGAACTTGGTCTCCCAATCCCGCCTCTTGAAGAGCAAAAGC
GGATTGTGGCGATTTTGGATGCGGCGTTTGAGAGGCTGGACCGTGCCAAAGAGAACGCCGAAGCCAATCTCCAAAACGCC
CGCGAGTTGTTTGATCGAACCCTCGAAAGAGTTTTCGCAGAACTAGTTGCTGTACACGCGACGATCAAGCTCGAGGAAGT
CACCTCAAAAATCACGAAAGGCTCTTCCCCTAAATGGCAGGGGTTTTCATATGTCGATTCGCCGGGAGTGCTTTTTGTCA
CAAGCGAAAACGTAGGCAAAAATGAGTTGCTCCTCGAAAAGACAAAGTATGTCGAAGAGGGTTTCAATCAGAAAGATAGG
AAGTCTATTCTTGCACCGGGTGACGTGCTGTCGAACATTGTCGGGGCATCTATTGGGCGCACCGCTGTTTTTGACTTGGA
CGCGGTTGCGAATATCAATCAAGCGGTTTGTTTGATGCGTTGCTTGCCGGAGCGACTTTCCCCCAAGTTTTTAAGCTTTT
TGCTGAACTCTCCTTACTTTAAGGCCCGCCTTCATGAAGGCGAAAGTAACATGGCTAGGGCAAATCTCAGCCTTGCCTTT
TTTCGGGAGTTCTTAGTGCCGTTGCCAGAATTGGAGGCACAAGAAAGGATCGTGCAGGAGATTGAAGAACTGGCCACGCA
TTCAGCAGAGTGCGAAACCAACTACCGAACCAAACTCACTGACATTGCCGACCTGCGTCAATCCCTCCTGCAAAAAGCCT
TTGCCGGGGAACTCACCTGA

Upstream 100 bases:

>100_bases
CCGAAGAAGCGCCGCTCCGCTCGCCCGAAGAGATCATCGAAGATATGCTGGCCCGAGACAAGGAGACAGCGGCAATACTG
GAAGACATCCGGGGGATGTT

Downstream 100 bases:

>100_bases
CGCTAACCTCCAACAATGGTTGAACATTCTTCCCTTGCCACAGAGACCGAAGCGGACACGCGCGCTCAGCGGATAGACCC
TGCGCTTGCCGCCGCCGGAT

Product: type I restriction enzyme specificity subunit, putative

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKAGWEVKPLGEVAKLHYGKALAESERSPNGTVPVYGANGVLGWSDHTLTEGPSLIVGRKGSAGEVNRVDGPFWPSDVTY
YTEHDPNRLDFDYFHYGLMTLNLPSLAKGVKPGINRNDVYELGLPIPPLEEQKRIVAILDAAFERLDRAKENAEANLQNA
RELFDRTLERVFAELVAVHATIKLEEVTSKITKGSSPKWQGFSYVDSPGVLFVTSENVGKNELLLEKTKYVEEGFNQKDR
KSILAPGDVLSNIVGASIGRTAVFDLDAVANINQAVCLMRCLPERLSPKFLSFLLNSPYFKARLHEGESNMARANLSLAF
FREFLVPLPELEAQERIVQEIEELATHSAECETNYRTKLTDIADLRQSLLQKAFAGELT

Sequences:

>Translated_379_residues
MKAGWEVKPLGEVAKLHYGKALAESERSPNGTVPVYGANGVLGWSDHTLTEGPSLIVGRKGSAGEVNRVDGPFWPSDVTY
YTEHDPNRLDFDYFHYGLMTLNLPSLAKGVKPGINRNDVYELGLPIPPLEEQKRIVAILDAAFERLDRAKENAEANLQNA
RELFDRTLERVFAELVAVHATIKLEEVTSKITKGSSPKWQGFSYVDSPGVLFVTSENVGKNELLLEKTKYVEEGFNQKDR
KSILAPGDVLSNIVGASIGRTAVFDLDAVANINQAVCLMRCLPERLSPKFLSFLLNSPYFKARLHEGESNMARANLSLAF
FREFLVPLPELEAQERIVQEIEELATHSAECETNYRTKLTDIADLRQSLLQKAFAGELT
>Mature_379_residues
MKAGWEVKPLGEVAKLHYGKALAESERSPNGTVPVYGANGVLGWSDHTLTEGPSLIVGRKGSAGEVNRVDGPFWPSDVTY
YTEHDPNRLDFDYFHYGLMTLNLPSLAKGVKPGINRNDVYELGLPIPPLEEQKRIVAILDAAFERLDRAKENAEANLQNA
RELFDRTLERVFAELVAVHATIKLEEVTSKITKGSSPKWQGFSYVDSPGVLFVTSENVGKNELLLEKTKYVEEGFNQKDR
KSILAPGDVLSNIVGASIGRTAVFDLDAVANINQAVCLMRCLPERLSPKFLSFLLNSPYFKARLHEGESNMARANLSLAF
FREFLVPLPELEAQERIVQEIEELATHSAECETNYRTKLTDIADLRQSLLQKAFAGELT

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 42153; Mature: 42153

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGWEVKPLGEVAKLHYGKALAESERSPNGTVPVYGANGVLGWSDHTLTEGPSLIVGRK
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEECC
GSAGEVNRVDGPFWPSDVTYYTEHDPNRLDFDYFHYGLMTLNLPSLAKGVKPGINRNDVY
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHEECCHHHHHCCCCCCCCCCEE
ELGLPIPPLEEQKRIVAILDAAFERLDRAKENAEANLQNARELFDRTLERVFAELVAVHA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKLEEVTSKITKGSSPKWQGFSYVDSPGVLFVTSENVGKNELLLEKTKYVEEGFNQKDR
HHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
KSILAPGDVLSNIVGASIGRTAVFDLDAVANINQAVCLMRCLPERLSPKFLSFLLNSPYF
HHCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
KARLHEGESNMARANLSLAFFREFLVPLPELEAQERIVQEIEELATHSAECETNYRTKLT
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DIADLRQSLLQKAFAGELT
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKAGWEVKPLGEVAKLHYGKALAESERSPNGTVPVYGANGVLGWSDHTLTEGPSLIVGRK
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEECC
GSAGEVNRVDGPFWPSDVTYYTEHDPNRLDFDYFHYGLMTLNLPSLAKGVKPGINRNDVY
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHEECCHHHHHCCCCCCCCCCEE
ELGLPIPPLEEQKRIVAILDAAFERLDRAKENAEANLQNARELFDRTLERVFAELVAVHA
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKLEEVTSKITKGSSPKWQGFSYVDSPGVLFVTSENVGKNELLLEKTKYVEEGFNQKDR
HHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
KSILAPGDVLSNIVGASIGRTAVFDLDAVANINQAVCLMRCLPERLSPKFLSFLLNSPYF
HHCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
KARLHEGESNMARANLSLAFFREFLVPLPELEAQERIVQEIEELATHSAECETNYRTKLT
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DIADLRQSLLQKAFAGELT
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]