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The map label for this gene is topA [H]

Identifier: 110679406

GI number: 110679406

Start: 2028915

End: 2031551

Strand: Reverse

Name: topA [H]

Synonym: RD1_2131

Alternate gene names: 110679406

Gene position: 2031551-2028915 (Counterclockwise)

Preceding gene: 110679407

Following gene: 110679405

Centisome position: 49.15

GC content: 60.3

Gene sequence:

>2637_bases
ATGCCGGTTGTTGTCGTCGAATCCCCTGCGAAAGCCAAGACAATCAATGCGTACCTGGGCGACGATTATACGGTGCTGGC
CTCCTACGGCCACGTGCGCGATCTGCCCCCGAAAGACGGCTCGGTCAATCCCGATGCGGATTTCGAGATGCTTTGGGAAG
TGGGCTATGACAGCCGCAAACACGTCAAGGCTATCGCGGATGCACTGAAGGAGGATAACGCCCTGATCCTCGCAACCGAC
CCCGACCGCGAGGGCGAAGCGATCAGTTGGCACCTTCAGGAAGCGCTGACAAAACGCAAATCCATCAAAAAGGATACCCC
CGTCAGCCGCGTGGTCTTTAACGCGATCACAAAGGCGGCCGTGACAGAAGCGATGCAGAACCCGCGTCAGGTGGATATGC
CGCTGGTTGAGGCATACCTTGCGCGGCGGGCGCTGGACTATCTGGTCGGGTTCAACCTCAGCCCCGTGTTGTGGCGCAAG
CTGCCCGGCGCAAAGTCCGCAGGCCGCGTGCAATCGGTGACCCTGCGCCTGATCGTTGAGCGCGAGATGGAAATCGAAGC
GTTCCGCGCGCGCGAATACTGGTCCGTCAAGGCACTGCTGGCCACGCCGCGGGGTCAGGAGTTCGAAGCCAAGCTGACCG
TTCTGGCGGGCAAGAAACTGGACCGCTTCGACATCGAAAACCAGACGCAGGCCGAGTTGGCCGTGCAGGCCATCACCAGC
CGCGATCTGAGCGTCAAATCGGTTGAGGCAAAACCGGCGAACCGAAACCCCTCGGCCCCCTTCATGACATCGACGCTGCA
ACAGGAAGCAAGCCGCAAGTTCGGCATGGGTGCGCGCCAGACAATGAGCACCGCGCAGCGGCTTTATGAGGCCGGCTACA
TTACCTACATGCGGACCGATGGCATCGACATGGCGCCCGAAGCGGTCACGGCCGCGCGCGATGCGATCAAAACGCTCTAC
GGGTCGAAATACGTGCCCGAAAAACCGCGTATCTACAAGAACAAGGCCAAGAACGCGCAGGAAGCGCATGAATGTGTCCG
CCCCACGGACATGATGACCAAGGCCGAGGACCTCAAGACCACGGATGCGGATCAACGCAAACTCTATGATCTGATCTGGA
AGCGTACCCTCGCCTGCCAGATGGAAGCCGCCCGGATGGAACGCACGACCGCCGAGATCGCGAGCGAAGACGGCCAGGTT
GGCCTCAGGGCGACCGGTCAGGTCATGTTGTTCGACGGTTTCCTGCGCGTCTACGAAGAAGGCCGCGACGATGTGGCGGT
GGTGGATGACGACGACAAACGCCTGCCACAGATTGATCAGGGCGACCGGCTGGACAAACGCACCGTCACCCCGGAGCAGC
ATTTCACCCAGCCACCGCCCAGATATACCGAAGCCACGCTGGTCAAACGCATGGAAGAATTGGGCATTGGTCGCCCCTCC
ACCTATGCTTCCGTGGTCACGACGATTCAGGACCGTGAATACGTTCGAAAGGACAAGAACCGCCTGATCCCGGAGGATAA
GGGGCGTCTCGTTACCGCCTTCCTGAGCAATTATTTCCGCAAATACATCGGATACGACTTCACTGCCGACCTTGAGGATC
AGCTCGACACGGTCAGCGCAGGAAATGCCGATTACAAGGAGGTTTTGGGGCGGTTCTGGCGCGATTTCTCCGCAGCGATT
GCCGAAACGGCAGATCTGCGCATCACCGAGGTGCTGGAAAAAATCAACGAGGTGCTGGAACCGCATCTGTTTCCGCCGAA
TGAAGACGGAAGCGATCCGCGCCTGTGTCCAAACTGCGGCGCGGGGCGACTGAGCATGCGCACGGCCCGTTCCGGCGGTG
CTTTCATCGGTTGCTCGAATTACCCCGAATGCCGATATACCCGCGCCTTTGGTCCGCCCGGCGAAGAGGATGACAGTGGC
ATCCCACCCGAAGGCAAGCTGCTGGGCGAGGACGGCGACGACAAGATTTATGCCTTCAAGGGACGTTTCGGCCCCTATGT
GCAGCGCGGTGAAGTCACCGAGGACAACAAGAAACCACCGCGCCAGTCCATCCCGAAAGACTGGCCGCCGGAGGATGTCG
ATCTCCCGCAGGCGCTCAAACTGCTGTCACTGCCACGCGAAATCGGGCCCCACCCCGAGGATGGGATCATGGTCTGGGCC
AATATCGGTCGCTATGGGCCCTACCTCAAACACGCGCCATCGACCTCTGACCGGGGGGGCACAAATGCCAATCTCGAGAG
CATCGAGGAGGTCTTTACCGTCGGCATGAACCGCGCGGTGCAGCTTCTGGCGGAAAAAGTCGCATCCCGTGGCAGGCGCG
GTGGCGCTGCGAAAACGCTGCGTGACCTTGGTGAACACCCCGAAGAGGGCGGCCCCGTCGCGATCATGGAGGGCAAATAC
GGACCCTACGTGAAATGGGACAAGATAAACGCCACCCTGCCCAAGGACAGCGACCCTGCCGAGCTGACCATGGAAGCCGC
CATCGCGCTGATCGACGAAAAAGCCGCCTCGAAAGGCAAGAAGCGGCGCAAGGCGGCCCCCAAGAAGGCAGCCGCCAAAA
AGACTGCGACAAAAAAGGCCGCGCCCAAAAAGACGGCTGCAAAGAAAAAGGCACCGGCAAAAAAAGCCAAGGAGTAA

Upstream 100 bases:

>100_bases
ACCGCGCTGAAATCAGCTTACCATTGAAAATAATGACTTGCGCACCACCTGTCTCACGGCCATAGACACAAGATATTTTT
GATCAGAGGTGCTTCAGTAC

Downstream 100 bases:

>100_bases
CGGACGTTTGAACGGCGTGCGCCCCAGGCCGTGTTAATATCTTGTGCTACGCGCATGCGTGAAATAAAACGTGGGCAACA
CCGGCCTGCGCTGTGGCAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 878; Mature: 877

Protein sequence:

>878_residues
MPVVVVESPAKAKTINAYLGDDYTVLASYGHVRDLPPKDGSVNPDADFEMLWEVGYDSRKHVKAIADALKEDNALILATD
PDREGEAISWHLQEALTKRKSIKKDTPVSRVVFNAITKAAVTEAMQNPRQVDMPLVEAYLARRALDYLVGFNLSPVLWRK
LPGAKSAGRVQSVTLRLIVEREMEIEAFRAREYWSVKALLATPRGQEFEAKLTVLAGKKLDRFDIENQTQAELAVQAITS
RDLSVKSVEAKPANRNPSAPFMTSTLQQEASRKFGMGARQTMSTAQRLYEAGYITYMRTDGIDMAPEAVTAARDAIKTLY
GSKYVPEKPRIYKNKAKNAQEAHECVRPTDMMTKAEDLKTTDADQRKLYDLIWKRTLACQMEAARMERTTAEIASEDGQV
GLRATGQVMLFDGFLRVYEEGRDDVAVVDDDDKRLPQIDQGDRLDKRTVTPEQHFTQPPPRYTEATLVKRMEELGIGRPS
TYASVVTTIQDREYVRKDKNRLIPEDKGRLVTAFLSNYFRKYIGYDFTADLEDQLDTVSAGNADYKEVLGRFWRDFSAAI
AETADLRITEVLEKINEVLEPHLFPPNEDGSDPRLCPNCGAGRLSMRTARSGGAFIGCSNYPECRYTRAFGPPGEEDDSG
IPPEGKLLGEDGDDKIYAFKGRFGPYVQRGEVTEDNKKPPRQSIPKDWPPEDVDLPQALKLLSLPREIGPHPEDGIMVWA
NIGRYGPYLKHAPSTSDRGGTNANLESIEEVFTVGMNRAVQLLAEKVASRGRRGGAAKTLRDLGEHPEEGGPVAIMEGKY
GPYVKWDKINATLPKDSDPAELTMEAAIALIDEKAASKGKKRRKAAPKKAAAKKTATKKAAPKKTAAKKKAPAKKAKE

Sequences:

>Translated_878_residues
MPVVVVESPAKAKTINAYLGDDYTVLASYGHVRDLPPKDGSVNPDADFEMLWEVGYDSRKHVKAIADALKEDNALILATD
PDREGEAISWHLQEALTKRKSIKKDTPVSRVVFNAITKAAVTEAMQNPRQVDMPLVEAYLARRALDYLVGFNLSPVLWRK
LPGAKSAGRVQSVTLRLIVEREMEIEAFRAREYWSVKALLATPRGQEFEAKLTVLAGKKLDRFDIENQTQAELAVQAITS
RDLSVKSVEAKPANRNPSAPFMTSTLQQEASRKFGMGARQTMSTAQRLYEAGYITYMRTDGIDMAPEAVTAARDAIKTLY
GSKYVPEKPRIYKNKAKNAQEAHECVRPTDMMTKAEDLKTTDADQRKLYDLIWKRTLACQMEAARMERTTAEIASEDGQV
GLRATGQVMLFDGFLRVYEEGRDDVAVVDDDDKRLPQIDQGDRLDKRTVTPEQHFTQPPPRYTEATLVKRMEELGIGRPS
TYASVVTTIQDREYVRKDKNRLIPEDKGRLVTAFLSNYFRKYIGYDFTADLEDQLDTVSAGNADYKEVLGRFWRDFSAAI
AETADLRITEVLEKINEVLEPHLFPPNEDGSDPRLCPNCGAGRLSMRTARSGGAFIGCSNYPECRYTRAFGPPGEEDDSG
IPPEGKLLGEDGDDKIYAFKGRFGPYVQRGEVTEDNKKPPRQSIPKDWPPEDVDLPQALKLLSLPREIGPHPEDGIMVWA
NIGRYGPYLKHAPSTSDRGGTNANLESIEEVFTVGMNRAVQLLAEKVASRGRRGGAAKTLRDLGEHPEEGGPVAIMEGKY
GPYVKWDKINATLPKDSDPAELTMEAAIALIDEKAASKGKKRRKAAPKKAAAKKTATKKAAPKKTAAKKKAPAKKAKE
>Mature_877_residues
PVVVVESPAKAKTINAYLGDDYTVLASYGHVRDLPPKDGSVNPDADFEMLWEVGYDSRKHVKAIADALKEDNALILATDP
DREGEAISWHLQEALTKRKSIKKDTPVSRVVFNAITKAAVTEAMQNPRQVDMPLVEAYLARRALDYLVGFNLSPVLWRKL
PGAKSAGRVQSVTLRLIVEREMEIEAFRAREYWSVKALLATPRGQEFEAKLTVLAGKKLDRFDIENQTQAELAVQAITSR
DLSVKSVEAKPANRNPSAPFMTSTLQQEASRKFGMGARQTMSTAQRLYEAGYITYMRTDGIDMAPEAVTAARDAIKTLYG
SKYVPEKPRIYKNKAKNAQEAHECVRPTDMMTKAEDLKTTDADQRKLYDLIWKRTLACQMEAARMERTTAEIASEDGQVG
LRATGQVMLFDGFLRVYEEGRDDVAVVDDDDKRLPQIDQGDRLDKRTVTPEQHFTQPPPRYTEATLVKRMEELGIGRPST
YASVVTTIQDREYVRKDKNRLIPEDKGRLVTAFLSNYFRKYIGYDFTADLEDQLDTVSAGNADYKEVLGRFWRDFSAAIA
ETADLRITEVLEKINEVLEPHLFPPNEDGSDPRLCPNCGAGRLSMRTARSGGAFIGCSNYPECRYTRAFGPPGEEDDSGI
PPEGKLLGEDGDDKIYAFKGRFGPYVQRGEVTEDNKKPPRQSIPKDWPPEDVDLPQALKLLSLPREIGPHPEDGIMVWAN
IGRYGPYLKHAPSTSDRGGTNANLESIEEVFTVGMNRAVQLLAEKVASRGRRGGAAKTLRDLGEHPEEGGPVAIMEGKYG
PYVKWDKINATLPKDSDPAELTMEAAIALIDEKAASKGKKRRKAAPKKAAAKKTATKKAAPKKTAAKKKAPAKKAKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=619, Percent_Identity=27.4636510500808, Blast_Score=151, Evalue=3e-36,
Organism=Homo sapiens, GI4507635, Length=555, Percent_Identity=25.2252252252252, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1787529, Length=667, Percent_Identity=41.8290854572714, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1788061, Length=534, Percent_Identity=26.5917602996255, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=520, Percent_Identity=26.7307692307692, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=560, Percent_Identity=26.25, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=489, Percent_Identity=26.1758691206544, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=639, Percent_Identity=26.4475743348983, Blast_Score=161, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640096, Length=590, Percent_Identity=22.0338983050847, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97621; Mature: 97490

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVVVESPAKAKTINAYLGDDYTVLASYGHVRDLPPKDGSVNPDADFEMLWEVGYDSRK
CCEEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHH
HVKAIADALKEDNALILATDPDREGEAISWHLQEALTKRKSIKKDTPVSRVVFNAITKAA
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTEAMQNPRQVDMPLVEAYLARRALDYLVGFNLSPVLWRKLPGAKSAGRVQSVTLRLIVE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHH
REMEIEAFRAREYWSVKALLATPRGQEFEAKLTVLAGKKLDRFDIENQTQAELAVQAITS
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHC
RDLSVKSVEAKPANRNPSAPFMTSTLQQEASRKFGMGARQTMSTAQRLYEAGYITYMRTD
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
GIDMAPEAVTAARDAIKTLYGSKYVPEKPRIYKNKAKNAQEAHECVRPTDMMTKAEDLKT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHCC
TDADQRKLYDLIWKRTLACQMEAARMERTTAEIASEDGQVGLRATGQVMLFDGFLRVYEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEHHHHHHHHHC
GRDDVAVVDDDDKRLPQIDQGDRLDKRTVTPEQHFTQPPPRYTEATLVKRMEELGIGRPS
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
TYASVVTTIQDREYVRKDKNRLIPEDKGRLVTAFLSNYFRKYIGYDFTADLEDQLDTVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
GNADYKEVLGRFWRDFSAAIAETADLRITEVLEKINEVLEPHLFPPNEDGSDPRLCPNCG
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AGRLSMRTARSGGAFIGCSNYPECRYTRAFGPPGEEDDSGIPPEGKLLGEDGDDKIYAFK
CCCEEEEECCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEE
GRFGPYVQRGEVTEDNKKPPRQSIPKDWPPEDVDLPQALKLLSLPREIGPHPEDGIMVWA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCCEEEEE
NIGRYGPYLKHAPSTSDRGGTNANLESIEEVFTVGMNRAVQLLAEKVASRGRRGGAAKTL
CCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RDLGEHPEEGGPVAIMEGKYGPYVKWDKINATLPKDSDPAELTMEAAIALIDEKAASKGK
HHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KRRKAAPKKAAAKKTATKKAAPKKTAAKKKAPAKKAKE
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PVVVVESPAKAKTINAYLGDDYTVLASYGHVRDLPPKDGSVNPDADFEMLWEVGYDSRK
CEEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHH
HVKAIADALKEDNALILATDPDREGEAISWHLQEALTKRKSIKKDTPVSRVVFNAITKAA
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTEAMQNPRQVDMPLVEAYLARRALDYLVGFNLSPVLWRKLPGAKSAGRVQSVTLRLIVE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHH
REMEIEAFRAREYWSVKALLATPRGQEFEAKLTVLAGKKLDRFDIENQTQAELAVQAITS
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHC
RDLSVKSVEAKPANRNPSAPFMTSTLQQEASRKFGMGARQTMSTAQRLYEAGYITYMRTD
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
GIDMAPEAVTAARDAIKTLYGSKYVPEKPRIYKNKAKNAQEAHECVRPTDMMTKAEDLKT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHCC
TDADQRKLYDLIWKRTLACQMEAARMERTTAEIASEDGQVGLRATGQVMLFDGFLRVYEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEHHHHHHHHHC
GRDDVAVVDDDDKRLPQIDQGDRLDKRTVTPEQHFTQPPPRYTEATLVKRMEELGIGRPS
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
TYASVVTTIQDREYVRKDKNRLIPEDKGRLVTAFLSNYFRKYIGYDFTADLEDQLDTVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
GNADYKEVLGRFWRDFSAAIAETADLRITEVLEKINEVLEPHLFPPNEDGSDPRLCPNCG
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AGRLSMRTARSGGAFIGCSNYPECRYTRAFGPPGEEDDSGIPPEGKLLGEDGDDKIYAFK
CCCEEEEECCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEE
GRFGPYVQRGEVTEDNKKPPRQSIPKDWPPEDVDLPQALKLLSLPREIGPHPEDGIMVWA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCCEEEEE
NIGRYGPYLKHAPSTSDRGGTNANLESIEEVFTVGMNRAVQLLAEKVASRGRRGGAAKTL
CCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RDLGEHPEEGGPVAIMEGKYGPYVKWDKINATLPKDSDPAELTMEAAIALIDEKAASKGK
HHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KRRKAAPKKAAAKKTATKKAAPKKTAAKKKAPAKKAKE
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA