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The map label for this gene is ogt [H]

Identifier: 110679356

GI number: 110679356

Start: 1984032

End: 1984487

Strand: Direct

Name: ogt [H]

Synonym: RD1_2075

Alternate gene names: 110679356

Gene position: 1984032-1984487 (Clockwise)

Preceding gene: 110679355

Following gene: 110679357

Centisome position: 48.0

GC content: 61.18

Gene sequence:

>456_bases
ATGTTGACATGCACGGTGGATACGCAATTCGGACGCCTGACCCTGAGGGAAGAGGCGGGCGCACTTGTCTCCGTCAGCTG
GGGCAGTGCAGCCCGAGAGGATGCATCAGAGCTTTTGCAGCGCGCCTCCTTGCAGGTTTCGGAATATGATCAAGGCGCGC
GCCAGCAGTTCGATCTGCCTGTTCGCATCCGGGGCAGCGCCTTTCAACGGGCGGTCTGCGACGCAATCTGCGCCATTCCG
TTTGGCGATACCTGCACCTACGGGGATATCGCCAGAGCGCTCGGCGTGCCTGCACAAGCGGTCGGACAGGCTTGCGGGGC
AAATCCGATCCCGATCATCATACCCTGTCACCGTGTCATGGGGGCCAAAGGGCTTACCGGGTTTTCGGGGGTAGGTGGGG
TGGAAACCAAAGTGGCGCTTTTGCGTCACGAAGGGGCGGCGGGTTTGTTGATCTAG

Upstream 100 bases:

>100_bases
GCGGGCGTTGTGACAGTGCGATGAGCTGACCCGAAAAGGCGCGGCCCGAAAGCGCAGCTTTCGGGTTGTTCCGAGGGCCG
TGATGCGCGACGGTCGGGCG

Downstream 100 bases:

>100_bases
AGCGTCTGACGAACAACCTGAAACATCGAGTATCACGTTACGCGTTGAAATCTTTGATTTCAGGCAGCGTTGCGAGTATC
AGGTTTTTTGCATGATGCTT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLTCTVDTQFGRLTLREEAGALVSVSWGSAAREDASELLQRASLQVSEYDQGARQQFDLPVRIRGSAFQRAVCDAICAIP
FGDTCTYGDIARALGVPAQAVGQACGANPIPIIIPCHRVMGAKGLTGFSGVGGVETKVALLRHEGAAGLLI

Sequences:

>Translated_151_residues
MLTCTVDTQFGRLTLREEAGALVSVSWGSAAREDASELLQRASLQVSEYDQGARQQFDLPVRIRGSAFQRAVCDAICAIP
FGDTCTYGDIARALGVPAQAVGQACGANPIPIIIPCHRVMGAKGLTGFSGVGGVETKVALLRHEGAAGLLI
>Mature_151_residues
MLTCTVDTQFGRLTLREEAGALVSVSWGSAAREDASELLQRASLQVSEYDQGARQQFDLPVRIRGSAFQRAVCDAICAIP
FGDTCTYGDIARALGVPAQAVGQACGANPIPIIIPCHRVMGAKGLTGFSGVGGVETKVALLRHEGAAGLLI

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=107, Percent_Identity=46.7289719626168, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI1788542, Length=90, Percent_Identity=47.7777777777778, Blast_Score=76, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=45.2380952380952, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=45.2380952380952, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=38.6363636363636, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 15773; Mature: 15773

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCTVDTQFGRLTLREEAGALVSVSWGSAAREDASELLQRASLQVSEYDQGARQQFDLP
CEEEEEECCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCHHHHCCCC
VRIRGSAFQRAVCDAICAIPFGDTCTYGDIARALGVPAQAVGQACGANPIPIIIPCHRVM
EEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEECHHHH
GAKGLTGFSGVGGVETKVALLRHEGAAGLLI
CCCCCCCCCCCCCCHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MLTCTVDTQFGRLTLREEAGALVSVSWGSAAREDASELLQRASLQVSEYDQGARQQFDLP
CEEEEEECCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCHHHHCCCC
VRIRGSAFQRAVCDAICAIPFGDTCTYGDIARALGVPAQAVGQACGANPIPIIIPCHRVM
EEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEECHHHH
GAKGLTGFSGVGGVETKVALLRHEGAAGLLI
CCCCCCCCCCCCCCHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]