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The map label for this gene is metZ [H]

Identifier: 110679226

GI number: 110679226

Start: 1848071

End: 1849252

Strand: Direct

Name: metZ [H]

Synonym: RD1_1939

Alternate gene names: 110679226

Gene position: 1848071-1849252 (Clockwise)

Preceding gene: 110679222

Following gene: 110679228

Centisome position: 44.71

GC content: 58.71

Gene sequence:

>1182_bases
ATGAAAAAAGACCTCAAACCGCGGACGCGCGCCGTGCATGCAGGCACAAGACGTAGCCAGTACGGCGAAGTGTCCGAAGC
GATTTTCCTGACGCAGGGCTTTGTCTATGAGACGGCAGAACAGGCCGAAGCGCGCTTTATCGAAGCGGGCCCGGATGAGT
TCATCTACGCGCGCTACGGAAACCCGACAGTTGCGATGTTCGAAGAGCGCATCGCCGCTCTGGAAGGGGCCGAAGACGCC
TTTGCCACTGCCTCGGGGATGGCCGCTGTATCAGGCGCGCTGACCTCCATGCTCAAGGCCGGCGATCATGTGGTATCGGC
GCGCGCCCTGTTCGGGTCTTGCCTCTATGTGCTCGAAGAAATCCTGACCCGCTATGGGGTTGAGGTGACTTTTGTGGACG
GCACCGACCTTGCGCAATGGGAAAGTGCCATCCGGGATGACACGCGCGCGGTTTTCTTCGAGTCCATCTCAAACCCCACT
CTGCAGGTCGTTGATATTGAGGCCGTAGCCAGCCTCGCGCATGCCAAAGGGGCGATGGTGTTGGTGGACAATGTATTTGC
CACCCCGGTCTGGTCCCGCGCTTTTGAACAAGGTGCAGACGTGGTGATCTATTCAACCACCAAACACATCGACGGACAGG
GGCGGACCCTTGGCGGCGTCATCCTTGGCACCACGGATTTCATCCGCAAGACCGTTGAACCCTACATGAAACACACCGGC
GGTTCTATGAGCCCGTTCACGGCCTGGGTCATGCTGAAGGGTCTTGAAACGCTGGATCTGCGGGTGCGCGCGCAGACTGA
GAATGCGATGGCCTTGGCCACGGCGCTGGAGGGGCATGAAAAGCTCAACCGCGTGATCTATCCCGGTCTTGCCAGTCACG
GCCAGCATGATCTGGTCCAGCGGCAGATGGGAAAAGGCGGCACGGTACTGGCCATTGATCTGGCCGGGGGCAAGGACGCG
GCCTTCCGGTTTTTGAATGCCATCGAGGTCGGGGTGATCTCCAACAACCTCGGTGACGCCAAAACCATTCTGACCCATCC
GGCAACCACGACGCACCAGCGTTTGCCGCAGGATCAGAAAGATGCACTGGGGATTTCGGCTGGCCTAATTCGGATCAGCG
TAGGGATTGAAGATCAGGAAGACCTGCGAAAGGACCTTTTTCAGGCTTTGCGTCATGTTTAG

Upstream 100 bases:

>100_bases
ACATTAAACACTCCGCTAAAAGGTCACTCGATGAGGAAACCCCGTCGCATTGACCGCGTATGGGGTTTTTTTGTGCCCAA
TTGGGCTGGAGAAGGCGAGA

Downstream 100 bases:

>100_bases
CGCGGCGTTGATAAACATATATAAAACAATGTTTTAGGTTGATTTTCCGGCGGTGCTGGCGCCTGGAGTGGGCAAATATG
CACCATGTATTTCCCTCTAT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKKDLKPRTRAVHAGTRRSQYGEVSEAIFLTQGFVYETAEQAEARFIEAGPDEFIYARYGNPTVAMFEERIAALEGAEDA
FATASGMAAVSGALTSMLKAGDHVVSARALFGSCLYVLEEILTRYGVEVTFVDGTDLAQWESAIRDDTRAVFFESISNPT
LQVVDIEAVASLAHAKGAMVLVDNVFATPVWSRAFEQGADVVIYSTTKHIDGQGRTLGGVILGTTDFIRKTVEPYMKHTG
GSMSPFTAWVMLKGLETLDLRVRAQTENAMALATALEGHEKLNRVIYPGLASHGQHDLVQRQMGKGGTVLAIDLAGGKDA
AFRFLNAIEVGVISNNLGDAKTILTHPATTTHQRLPQDQKDALGISAGLIRISVGIEDQEDLRKDLFQALRHV

Sequences:

>Translated_393_residues
MKKDLKPRTRAVHAGTRRSQYGEVSEAIFLTQGFVYETAEQAEARFIEAGPDEFIYARYGNPTVAMFEERIAALEGAEDA
FATASGMAAVSGALTSMLKAGDHVVSARALFGSCLYVLEEILTRYGVEVTFVDGTDLAQWESAIRDDTRAVFFESISNPT
LQVVDIEAVASLAHAKGAMVLVDNVFATPVWSRAFEQGADVVIYSTTKHIDGQGRTLGGVILGTTDFIRKTVEPYMKHTG
GSMSPFTAWVMLKGLETLDLRVRAQTENAMALATALEGHEKLNRVIYPGLASHGQHDLVQRQMGKGGTVLAIDLAGGKDA
AFRFLNAIEVGVISNNLGDAKTILTHPATTTHQRLPQDQKDALGISAGLIRISVGIEDQEDLRKDLFQALRHV
>Mature_393_residues
MKKDLKPRTRAVHAGTRRSQYGEVSEAIFLTQGFVYETAEQAEARFIEAGPDEFIYARYGNPTVAMFEERIAALEGAEDA
FATASGMAAVSGALTSMLKAGDHVVSARALFGSCLYVLEEILTRYGVEVTFVDGTDLAQWESAIRDDTRAVFFESISNPT
LQVVDIEAVASLAHAKGAMVLVDNVFATPVWSRAFEQGADVVIYSTTKHIDGQGRTLGGVILGTTDFIRKTVEPYMKHTG
GSMSPFTAWVMLKGLETLDLRVRAQTENAMALATALEGHEKLNRVIYPGLASHGQHDLVQRQMGKGGTVLAIDLAGGKDA
AFRFLNAIEVGVISNNLGDAKTILTHPATTTHQRLPQDQKDALGISAGLIRISVGIEDQEDLRKDLFQALRHV

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=33.9378238341969, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=30.8290155440415, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI25453487, Length=349, Percent_Identity=30.0859598853868, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=31.0077519379845, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=24.6192893401015, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=36.5284974093264, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=388, Percent_Identity=36.340206185567, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=342, Percent_Identity=33.6257309941521, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=34.4387755102041, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=411, Percent_Identity=32.8467153284672, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=25.0614250614251, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=401, Percent_Identity=27.431421446384, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=282, Percent_Identity=25.886524822695, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=32.8125, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42601; Mature: 42601

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLKPRTRAVHAGTRRSQYGEVSEAIFLTQGFVYETAEQAEARFIEAGPDEFIYARYG
CCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEEEEC
NPTVAMFEERIAALEGAEDAFATASGMAAVSGALTSMLKAGDHVVSARALFGSCLYVLEE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILTRYGVEVTFVDGTDLAQWESAIRDDTRAVFFESISNPTLQVVDIEAVASLAHAKGAMV
HHHHHCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEE
LVDNVFATPVWSRAFEQGADVVIYSTTKHIDGQGRTLGGVILGTTDFIRKTVEPYMKHTG
EECCHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEECCHHHHHHHHHHHHHHCC
GSMSPFTAWVMLKGLETLDLRVRAQTENAMALATALEGHEKLNRVIYPGLASHGQHDLVQ
CCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
RQMGKGGTVLAIDLAGGKDAAFRFLNAIEVGVISNNLGDAKTILTHPATTTHQRLPQDQK
HHHCCCCCEEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCHHHHCCCCHH
DALGISAGLIRISVGIEDQEDLRKDLFQALRHV
HHCCCEECEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKDLKPRTRAVHAGTRRSQYGEVSEAIFLTQGFVYETAEQAEARFIEAGPDEFIYARYG
CCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEEEEC
NPTVAMFEERIAALEGAEDAFATASGMAAVSGALTSMLKAGDHVVSARALFGSCLYVLEE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILTRYGVEVTFVDGTDLAQWESAIRDDTRAVFFESISNPTLQVVDIEAVASLAHAKGAMV
HHHHHCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEE
LVDNVFATPVWSRAFEQGADVVIYSTTKHIDGQGRTLGGVILGTTDFIRKTVEPYMKHTG
EECCHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCEECEEEECCHHHHHHHHHHHHHHCC
GSMSPFTAWVMLKGLETLDLRVRAQTENAMALATALEGHEKLNRVIYPGLASHGQHDLVQ
CCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
RQMGKGGTVLAIDLAGGKDAAFRFLNAIEVGVISNNLGDAKTILTHPATTTHQRLPQDQK
HHHCCCCCEEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCHHHHCCCCHH
DALGISAGLIRISVGIEDQEDLRKDLFQALRHV
HHCCCEECEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]