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The map label for this gene is gst [H]

Identifier: 110679224

GI number: 110679224

Start: 1846137

End: 1846841

Strand: Reverse

Name: gst [H]

Synonym: RD1_1937

Alternate gene names: 110679224

Gene position: 1846841-1846137 (Counterclockwise)

Preceding gene: 110679225

Following gene: 110679223

Centisome position: 44.68

GC content: 56.6

Gene sequence:

>705_bases
ATGAGCCAGCCTATTGATCTGTTTTTCTGGCCCACGCCAAACGGCTGGAAGATTTCGATAGCGCTTGAGGAAATGGGCCT
GCCCTATGCGACACATCTCATTGATATCGGGGCTGGCGATCAATTCGCTTCCGACTTCCTGAAAATTGCACCAAACAACA
GGATGCCCGCAATCATCGACCCTGAGGGGCCAGATGGTGCGCCGGTTTCGGTTTTCGAATCCGGTGCCATCCTGCAATAC
CTCGCCGGGAAAACCGGCCTTTTCGGTGGCCAGACCGCCCGCGACAGGATCGCTGTGACCGAGTGGCTGATGTGGCAGAT
GGGCGGCCTTGGCCCTATGGCCGGTCAGGCGCATCATTTCTTGAAATATGCGCCGGCAATGGACCCGCCCCATGATCTGC
CCTATGCGAAAAACAGGTACAGAACCGAGGTTGAAAGGCTATACGGCGTGCTGGATCGTCAACTGGCCCGCCATGAATAT
GTCGCCGGTGATTTCTACTCGATTGCGGATATGTCGATTTGGGGTTGGGCATCTCTTTGGGAAGGTCAGCAGCAATCCCT
TGACGATAAACCACATCTTGAACGCTGGCTTGAGACGGTGAGTGCGCGCCCCGGTGTCATCGCAGGCCGCGCGCTGCATG
CGGACAAACGCGGGGATTTCCGCAAGTACAAGGCCGCGCAGGATCAACTGTTCTCAAAACCCTGA

Upstream 100 bases:

>100_bases
CAAATTCGCATCCCTCAGGATTTTGACTTGTCGCATCGCACACATCAGGTAGGCTCCGGCGTGCGCCTGCCCAATGCGAC
CTGCCTGCGGAGTGACAAGA

Downstream 100 bases:

>100_bases
TTTCCGCAGAGTTCCTGATAAACAATACTTTTCGTCTCGATTTTCTTTGCAATGCCGGGCGTTTTCTCCAAAAGTCCCGC
AATGACAGCAAAGTACAGAG

Product: glutathione S-transferase, putative

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSQPIDLFFWPTPNGWKISIALEEMGLPYATHLIDIGAGDQFASDFLKIAPNNRMPAIIDPEGPDGAPVSVFESGAILQY
LAGKTGLFGGQTARDRIAVTEWLMWQMGGLGPMAGQAHHFLKYAPAMDPPHDLPYAKNRYRTEVERLYGVLDRQLARHEY
VAGDFYSIADMSIWGWASLWEGQQQSLDDKPHLERWLETVSARPGVIAGRALHADKRGDFRKYKAAQDQLFSKP

Sequences:

>Translated_234_residues
MSQPIDLFFWPTPNGWKISIALEEMGLPYATHLIDIGAGDQFASDFLKIAPNNRMPAIIDPEGPDGAPVSVFESGAILQY
LAGKTGLFGGQTARDRIAVTEWLMWQMGGLGPMAGQAHHFLKYAPAMDPPHDLPYAKNRYRTEVERLYGVLDRQLARHEY
VAGDFYSIADMSIWGWASLWEGQQQSLDDKPHLERWLETVSARPGVIAGRALHADKRGDFRKYKAAQDQLFSKP
>Mature_233_residues
SQPIDLFFWPTPNGWKISIALEEMGLPYATHLIDIGAGDQFASDFLKIAPNNRMPAIIDPEGPDGAPVSVFESGAILQYL
AGKTGLFGGQTARDRIAVTEWLMWQMGGLGPMAGQAHHFLKYAPAMDPPHDLPYAKNRYRTEVERLYGVLDRQLARHEYV
AGDFYSIADMSIWGWASLWEGQQQSLDDKPHLERWLETVSARPGVIAGRALHADKRGDFRKYKAAQDQLFSKP

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=218, Percent_Identity=47.7064220183486, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082195, Length=226, Percent_Identity=40.2654867256637, Blast_Score=150, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26201; Mature: 26070

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPIDLFFWPTPNGWKISIALEEMGLPYATHLIDIGAGDQFASDFLKIAPNNRMPAIID
CCCCCEEEEECCCCCEEEEEEHHHHCCCHHHHEEECCCCHHHHHHHHHCCCCCCCCEEEC
PEGPDGAPVSVFESGAILQYLAGKTGLFGGQTARDRIAVTEWLMWQMGGLGPMAGQAHHF
CCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LKYAPAMDPPHDLPYAKNRYRTEVERLYGVLDRQLARHEYVAGDFYSIADMSIWGWASLW
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
EGQQQSLDDKPHLERWLETVSARPGVIAGRALHADKRGDFRKYKAAQDQLFSKP
CCHHHCCCCCHHHHHHHHHHCCCCCEEECCEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQPIDLFFWPTPNGWKISIALEEMGLPYATHLIDIGAGDQFASDFLKIAPNNRMPAIID
CCCCEEEEECCCCCEEEEEEHHHHCCCHHHHEEECCCCHHHHHHHHHCCCCCCCCEEEC
PEGPDGAPVSVFESGAILQYLAGKTGLFGGQTARDRIAVTEWLMWQMGGLGPMAGQAHHF
CCCCCCCCEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LKYAPAMDPPHDLPYAKNRYRTEVERLYGVLDRQLARHEYVAGDFYSIADMSIWGWASLW
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
EGQQQSLDDKPHLERWLETVSARPGVIAGRALHADKRGDFRKYKAAQDQLFSKP
CCHHHCCCCCHHHHHHHHHHCCCCCEEECCEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]