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The map label for this gene is puuA [H]

Identifier: 110679182

GI number: 110679182

Start: 1808136

End: 1809494

Strand: Reverse

Name: puuA [H]

Synonym: RD1_1892

Alternate gene names: 110679182

Gene position: 1809494-1808136 (Counterclockwise)

Preceding gene: 110679183

Following gene: 110679181

Centisome position: 43.78

GC content: 60.63

Gene sequence:

>1359_bases
ATGGCCGACTGGATGACAGAACTGCCGGATGCTGCGCGCAGCTATCTGGAGGGGCACCGGCTTGACGAGGTCGAGTGCAT
CATTTCGGACCTGCCCGGCATTGCGCGCGGCAAGGCCGTACCTGCATTGAAATTCCAACGGCAGGATTCCTTCCACCTGC
CCGACAGCATCTTTTACCAGACCATCACCGGCGGCTGGGGCGAAGCGGCGGGGGATGATGGTTTCATCGAACGCGACATG
ATCCTCAAGCCCGACATGAGCACCGCGACCGCCGCCCCATGGACCGGCGACTGGACGTTGCAGGTGATCCATGACGCCTT
TGATCGCGCCGGGGAGCCTGTCCCCTTCAGTCCGCGCAACGTGTTGAAACGCGTCTGCAAGCTTTATCAGGACAAAGGCT
GGTCCCCGGTTGTCGCCCCCGAGATGGAGTTTTTTCTGGTGGCGCGCAACATTGACCCGGCCCATGACATCAAGCCGATG
ATGGGCCGGTCGGGGCGTCCTGCGGCGGCGCGGCAGGCCTATTCCATGACAGCCGTGGACGAATTCGGCCCCGTCATTGA
TGACATTTACGATTTCGCCGAGGCGCAGGGCTTCGAGATCGACGGCATTACGCAGGAAGGCGGCGCAGGTCAGTTGGAGA
TCAACCTGCTGCACGGAGAGCCGGTGAAGCTCGCCGATGAAATCTTTTACTTCAAACGGCTGATCCGCGAGGCGGCGTTG
CGCCACGATTGTTTCGCCACTTTCATGGCCAAACCCATCGCGGATGAACCGGGCAGTGCCATGCATATCCACCACTCCGT
TCTGAACATGGAGACCGGGCAGAATGTGTTTTCTGACGCGGATGGTGCTGAAACCGATCTGTTCCGCCACTTCATTGCCG
GTCTGCAAAACCACATGCCCGCAGCGCTCGCGGTGTTTGCACCTTACGTCAACAGCTATCGCCGCTACGTCAAAGATCAC
GCAGCACCGATCAACCTGAACTGGGGACGGGACAATCGCACCACGGGCATCCGCATACCCCTGTCCAGCCCCGCCGCGCG
GCGCGTGGAAAACCGGCTGGCCGGGATGGATTGCAACCCCTACCTGGGCATCGCCGCCAGCCTCGCCTGCGGCTATCTCG
GCATGACCCGGCAAGAGGAACCGCAGCCGGAGTTCAAGGGCGACGCTTATGACGGGGATGGGGACATTCCGCGTGATCTG
GGCTCTGCCCTGAATATGTTTGACGAAGCAGAGGCGATCCACAAGGTTCTGGGACCAGATTTCGCCCGCGTTTATTCCAT
CGTGAAACGCGCGGAATACGAGGAGTTCCTGCAAGTCATCTCGCCCTGGGAACGCGAGCATCTGTTGCTGAACGTCTAG

Upstream 100 bases:

>100_bases
AGGACCTCTTGGCACGTGCAATGTCCAAGCTCGATCAGCCGAGCGACCGCATGGACGTCGCCAACAAAATGGCTGCATTT
TTCAAGAAAGAGAGGGCCGC

Downstream 100 bases:

>100_bases
GCAACGTCTGGCCGTCATATGGATTTGCTTTACACAAACGACACTGCGGGCCAGTATCCGCCAAGCTGGTATGCGGCAAC
CGCAATGCCGCCTGCCCCCT

Product: glutamine synthetase, putative

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MADWMTELPDAARSYLEGHRLDEVECIISDLPGIARGKAVPALKFQRQDSFHLPDSIFYQTITGGWGEAAGDDGFIERDM
ILKPDMSTATAAPWTGDWTLQVIHDAFDRAGEPVPFSPRNVLKRVCKLYQDKGWSPVVAPEMEFFLVARNIDPAHDIKPM
MGRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLLHGEPVKLADEIFYFKRLIREAAL
RHDCFATFMAKPIADEPGSAMHIHHSVLNMETGQNVFSDADGAETDLFRHFIAGLQNHMPAALAVFAPYVNSYRRYVKDH
AAPINLNWGRDNRTTGIRIPLSSPAARRVENRLAGMDCNPYLGIAASLACGYLGMTRQEEPQPEFKGDAYDGDGDIPRDL
GSALNMFDEAEAIHKVLGPDFARVYSIVKRAEYEEFLQVISPWEREHLLLNV

Sequences:

>Translated_452_residues
MADWMTELPDAARSYLEGHRLDEVECIISDLPGIARGKAVPALKFQRQDSFHLPDSIFYQTITGGWGEAAGDDGFIERDM
ILKPDMSTATAAPWTGDWTLQVIHDAFDRAGEPVPFSPRNVLKRVCKLYQDKGWSPVVAPEMEFFLVARNIDPAHDIKPM
MGRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLLHGEPVKLADEIFYFKRLIREAAL
RHDCFATFMAKPIADEPGSAMHIHHSVLNMETGQNVFSDADGAETDLFRHFIAGLQNHMPAALAVFAPYVNSYRRYVKDH
AAPINLNWGRDNRTTGIRIPLSSPAARRVENRLAGMDCNPYLGIAASLACGYLGMTRQEEPQPEFKGDAYDGDGDIPRDL
GSALNMFDEAEAIHKVLGPDFARVYSIVKRAEYEEFLQVISPWEREHLLLNV
>Mature_451_residues
ADWMTELPDAARSYLEGHRLDEVECIISDLPGIARGKAVPALKFQRQDSFHLPDSIFYQTITGGWGEAAGDDGFIERDMI
LKPDMSTATAAPWTGDWTLQVIHDAFDRAGEPVPFSPRNVLKRVCKLYQDKGWSPVVAPEMEFFLVARNIDPAHDIKPMM
GRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLLHGEPVKLADEIFYFKRLIREAALR
HDCFATFMAKPIADEPGSAMHIHHSVLNMETGQNVFSDADGAETDLFRHFIAGLQNHMPAALAVFAPYVNSYRRYVKDHA
APINLNWGRDNRTTGIRIPLSSPAARRVENRLAGMDCNPYLGIAASLACGYLGMTRQEEPQPEFKGDAYDGDGDIPRDLG
SALNMFDEAEAIHKVLGPDFARVYSIVKRAEYEEFLQVISPWEREHLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=438, Percent_Identity=25.5707762557078, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI87081870, Length=447, Percent_Identity=34.8993288590604, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1790301, Length=426, Percent_Identity=30.2816901408451, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50347; Mature: 50216

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWMTELPDAARSYLEGHRLDEVECIISDLPGIARGKAVPALKFQRQDSFHLPDSIFYQ
CCCHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCHHHHHH
TITGGWGEAAGDDGFIERDMILKPDMSTATAAPWTGDWTLQVIHDAFDRAGEPVPFSPRN
HHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
VLKRVCKLYQDKGWSPVVAPEMEFFLVARNIDPAHDIKPMMGRSGRPAAARQAYSMTAVD
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCHHHHCCCCCCHHHHHHHHHHHHH
EFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLLHGEPVKLADEIFYFKRLIREAAL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RHDCFATFMAKPIADEPGSAMHIHHSVLNMETGQNVFSDADGAETDLFRHFIAGLQNHMP
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
AALAVFAPYVNSYRRYVKDHAAPINLNWGRDNRTTGIRIPLSSPAARRVENRLAGMDCNP
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCC
YLGIAASLACGYLGMTRQEEPQPEFKGDAYDGDGDIPRDLGSALNMFDEAEAIHKVLGPD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FARVYSIVKRAEYEEFLQVISPWEREHLLLNV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
ADWMTELPDAARSYLEGHRLDEVECIISDLPGIARGKAVPALKFQRQDSFHLPDSIFYQ
CCHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCHHHHHH
TITGGWGEAAGDDGFIERDMILKPDMSTATAAPWTGDWTLQVIHDAFDRAGEPVPFSPRN
HHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
VLKRVCKLYQDKGWSPVVAPEMEFFLVARNIDPAHDIKPMMGRSGRPAAARQAYSMTAVD
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCHHHHCCCCCCHHHHHHHHHHHHH
EFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLLHGEPVKLADEIFYFKRLIREAAL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RHDCFATFMAKPIADEPGSAMHIHHSVLNMETGQNVFSDADGAETDLFRHFIAGLQNHMP
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
AALAVFAPYVNSYRRYVKDHAAPINLNWGRDNRTTGIRIPLSSPAARRVENRLAGMDCNP
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCC
YLGIAASLACGYLGMTRQEEPQPEFKGDAYDGDGDIPRDLGSALNMFDEAEAIHKVLGPD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FARVYSIVKRAEYEEFLQVISPWEREHLLLNV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]