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The map label for this gene is gloA [H]

Identifier: 110679162

GI number: 110679162

Start: 1792224

End: 1792652

Strand: Reverse

Name: gloA [H]

Synonym: RD1_1872

Alternate gene names: 110679162

Gene position: 1792652-1792224 (Counterclockwise)

Preceding gene: 110679166

Following gene: 110679161

Centisome position: 43.37

GC content: 55.71

Gene sequence:

>429_bases
ATGACACTGAAATACCTGCACACGATGGTCCGCGTAAAGGACCTCGAAAAATCCATCGCTTTTTACGAACTGTTGGGCCT
GAAGCAAACGCGGCGCATGGACAGCGAGAGCGGTCGCTTCAGTCTTGTGTTCATGGCACCACCCGGGCAAGAGGAATGCC
CGGTGGAATTGACATACAACTGGGATGGTGACGAGGGTCTGCCCAGCGACAGCCGCCATTTCGGGCATCTCGCCTACCGG
GTGGGCAACATCTATGAGGTGTGCCAGCATTTGATGGACAACGGCGTTACAATCAACCGCCCCCCGCGTGATGGTCACAT
GGCTTTTGTGCGCTCACCTGATAATGTTTCGATCGAGCTTTTGCAGGAAGGCGACCACCTTGCGCCGGCTGAGCCATGGG
CAAGCATGGAAAATACGGGGCATTGGTAA

Upstream 100 bases:

>100_bases
GCGGGTGACATGCCGGCGTGTTCACGTCTGCGGGATGATGCTGACGTCGCGGGCTGAGCATGTTAAGCTGCAATCGTAGT
ACACATAAACGGAATGCCAG

Downstream 100 bases:

>100_bases
GAGGAGCCACTCTTGCCGCGCTCATGCTGTTCTCTGCGCCAGCCGCTCTGGCGCAGAATTGTCGCTTGGCGTTGCTCCTT
GCGATAGATGTGTCGAGCTC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MTLKYLHTMVRVKDLEKSIAFYELLGLKQTRRMDSESGRFSLVFMAPPGQEECPVELTYNWDGDEGLPSDSRHFGHLAYR
VGNIYEVCQHLMDNGVTINRPPRDGHMAFVRSPDNVSIELLQEGDHLAPAEPWASMENTGHW

Sequences:

>Translated_142_residues
MTLKYLHTMVRVKDLEKSIAFYELLGLKQTRRMDSESGRFSLVFMAPPGQEECPVELTYNWDGDEGLPSDSRHFGHLAYR
VGNIYEVCQHLMDNGVTINRPPRDGHMAFVRSPDNVSIELLQEGDHLAPAEPWASMENTGHW
>Mature_141_residues
TLKYLHTMVRVKDLEKSIAFYELLGLKQTRRMDSESGRFSLVFMAPPGQEECPVELTYNWDGDEGLPSDSRHFGHLAYRV
GNIYEVCQHLMDNGVTINRPPRDGHMAFVRSPDNVSIELLQEGDHLAPAEPWASMENTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=33.8028169014084, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1787940, Length=130, Percent_Identity=39.2307692307692, Blast_Score=86, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=147, Percent_Identity=31.2925170068027, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=31.7241379310345, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16233; Mature: 16101

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKYLHTMVRVKDLEKSIAFYELLGLKQTRRMDSESGRFSLVFMAPPGQEECPVELTYN
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
WDGDEGLPSDSRHFGHLAYRVGNIYEVCQHLMDNGVTINRPPRDGHMAFVRSPDNVSIEL
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCEEEE
LQEGDHLAPAEPWASMENTGHW
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLKYLHTMVRVKDLEKSIAFYELLGLKQTRRMDSESGRFSLVFMAPPGQEECPVELTYN
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEE
WDGDEGLPSDSRHFGHLAYRVGNIYEVCQHLMDNGVTINRPPRDGHMAFVRSPDNVSIEL
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCEEEE
LQEGDHLAPAEPWASMENTGHW
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]