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The map label for this gene is appA [H]

Identifier: 110679149

GI number: 110679149

Start: 1779379

End: 1780902

Strand: Direct

Name: appA [H]

Synonym: RD1_1859

Alternate gene names: 110679149

Gene position: 1779379-1780902 (Clockwise)

Preceding gene: 110679148

Following gene: 110679151

Centisome position: 43.05

GC content: 61.48

Gene sequence:

>1524_bases
ATGTTACTTATCAAACGCATGGCCGCTGCTGCGGTATTTGCGCTGGGGCTGGCCGGTCCCACGCTTGCGCAGGAGGGCAG
TTTTACCATCTCCGTCACCTTTGGGCCAACGGCCGAGGTGCCCGACCCGCGGGCAGGCTACAACGGGTGGATGTCAAATC
AGACCGGTGTGACTGAAACCCTGATGGGCATTGATTATGATTTGAACCTCTACCCGCGCCTTGCCGAAAACATCGCGCAG
ATCGCGCCCACGCAGTGGCGTGTCACCCTGCGCGAGGGTCTTCAATTCCATGACGGATCGCCGGTGACGGCGCAGGCCGT
CATTGACGCGATTGCTCCGATTTCTCAGGCGGATCATCCAGGTTACAACGCCCGCATTGCCCGCGTGCTGGATCTGGCGG
GAATGTCGGCGCAGGATGACAGGACTGTGGTGTTTGAAACAAACAGCCCGAATGCCGCCTTCCAGTGGAGCCTGTCGGAC
CCCGGTGTGGCCATACTCGGCGCAGCCTCCGAGGCGTTTCCGATCAATGCGACCGGGCCTTATGTGTTCCGTGAGGCCAT
TGTGGATCAGCTCTACCGCGTTGAGGCCAATCCCGACTACCGCCTTGGCGCGCCGGGTTTTGAGGAAGTGCGCGTTGCTG
CCTCACCGGACCCGGCAGCCGCAGCCCTCGCGTTTGAAGCGGGGGAGGTTGATCTGGTCATCAACTATCCGGAAACCGAT
TTTGAACGGATACAGGAGACGGGTGCGCTTGGCTTCACGGCCCCCACGGCACGGCTTTACTTCTATTCGATGAACACGGC
ATCCGGTCCGCTGGCCAACCCTTTGATCCGCCGCGCCGTTTCACTGGCGATTGATCGTGATGGCATCGTCGAGGCGGTGT
TGTCGGGCGTGGGCGGCGTGCCTGCGGGCACCATCTACCCGGCGGGCAAAAGCTGGGCGGCTGACATCGCCCCCACCTAT
GACCCCGCGCGCGCAGAGGCCTTGTTGAGCGAGGCCGGTGCCGTCAAACGGGGCGGGCGCTGGATGCTGGACGGTGAACC
GATTGAGATCGACATCCTGACCTATTCCTCGCGTGCCGCGCTTCCCCCGACAGCCGAAATCACGCAGGCGTTCCTGCAGC
AGATCGGCATTACGGCGCGCGTCCGGGTTGGCGAATATGGCGCCAGCAATGATGCGATGCTGGCCGGGGAGGGGGATATG
TTTCTGCAGGCTTGGGTGATGCTGCCGCAGGGTGATCCCGGTTCGGTTCTCGGCTTTTTGCTGGCCAGCGATGGGGGGGC
GAATTCGGGGCGCTATGCGAATCCCGCCTTGGACGAACTATTGATAAAGGGGCAAACCACCTTTGAGCAGTCCGAGCGTG
AGCGGATTTATGATGAGGTTCAGCAGATCATCGCGACAGATGTGCCGCTGATCCCCGTCTTTCACGTCAGTCAGGCCAAT
GTGGCGCGCGCGGGCTTGACGGGCTATGCCGTTCATCCGACAGAGACTTACTGGCTTACACATGAGACCGCTTTGAGCGA
ATGA

Upstream 100 bases:

>100_bases
GCACTCCGTGCCCTGTCGCGTGCCGAGGCGCAAACACTGGCGCGTGACCGGCCTTTGATCCGCGAAATTCACACACCTTA
AGCTATCAGGGAAACCACAT

Downstream 100 bases:

>100_bases
CGATTAATGCAACGGGCCTGAGCGCTGAGCGCTCGGGCCGCACGATCCTGCATCCGACGCATCTGCGGATGGCGCCCGGC
GACCGCGTCGGGCTTGTTGG

Product: dipeptide ABC transporter, substrate-binding protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLLIKRMAAAAVFALGLAGPTLAQEGSFTISVTFGPTAEVPDPRAGYNGWMSNQTGVTETLMGIDYDLNLYPRLAENIAQ
IAPTQWRVTLREGLQFHDGSPVTAQAVIDAIAPISQADHPGYNARIARVLDLAGMSAQDDRTVVFETNSPNAAFQWSLSD
PGVAILGAASEAFPINATGPYVFREAIVDQLYRVEANPDYRLGAPGFEEVRVAASPDPAAAALAFEAGEVDLVINYPETD
FERIQETGALGFTAPTARLYFYSMNTASGPLANPLIRRAVSLAIDRDGIVEAVLSGVGGVPAGTIYPAGKSWAADIAPTY
DPARAEALLSEAGAVKRGGRWMLDGEPIEIDILTYSSRAALPPTAEITQAFLQQIGITARVRVGEYGASNDAMLAGEGDM
FLQAWVMLPQGDPGSVLGFLLASDGGANSGRYANPALDELLIKGQTTFEQSERERIYDEVQQIIATDVPLIPVFHVSQAN
VARAGLTGYAVHPTETYWLTHETALSE

Sequences:

>Translated_507_residues
MLLIKRMAAAAVFALGLAGPTLAQEGSFTISVTFGPTAEVPDPRAGYNGWMSNQTGVTETLMGIDYDLNLYPRLAENIAQ
IAPTQWRVTLREGLQFHDGSPVTAQAVIDAIAPISQADHPGYNARIARVLDLAGMSAQDDRTVVFETNSPNAAFQWSLSD
PGVAILGAASEAFPINATGPYVFREAIVDQLYRVEANPDYRLGAPGFEEVRVAASPDPAAAALAFEAGEVDLVINYPETD
FERIQETGALGFTAPTARLYFYSMNTASGPLANPLIRRAVSLAIDRDGIVEAVLSGVGGVPAGTIYPAGKSWAADIAPTY
DPARAEALLSEAGAVKRGGRWMLDGEPIEIDILTYSSRAALPPTAEITQAFLQQIGITARVRVGEYGASNDAMLAGEGDM
FLQAWVMLPQGDPGSVLGFLLASDGGANSGRYANPALDELLIKGQTTFEQSERERIYDEVQQIIATDVPLIPVFHVSQAN
VARAGLTGYAVHPTETYWLTHETALSE
>Mature_507_residues
MLLIKRMAAAAVFALGLAGPTLAQEGSFTISVTFGPTAEVPDPRAGYNGWMSNQTGVTETLMGIDYDLNLYPRLAENIAQ
IAPTQWRVTLREGLQFHDGSPVTAQAVIDAIAPISQADHPGYNARIARVLDLAGMSAQDDRTVVFETNSPNAAFQWSLSD
PGVAILGAASEAFPINATGPYVFREAIVDQLYRVEANPDYRLGAPGFEEVRVAASPDPAAAALAFEAGEVDLVINYPETD
FERIQETGALGFTAPTARLYFYSMNTASGPLANPLIRRAVSLAIDRDGIVEAVLSGVGGVPAGTIYPAGKSWAADIAPTY
DPARAEALLSEAGAVKRGGRWMLDGEPIEIDILTYSSRAALPPTAEITQAFLQQIGITARVRVGEYGASNDAMLAGEGDM
FLQAWVMLPQGDPGSVLGFLLASDGGANSGRYANPALDELLIKGQTTFEQSERERIYDEVQQIIATDVPLIPVFHVSQAN
VARAGLTGYAVHPTETYWLTHETALSE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=430, Percent_Identity=24.4186046511628, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081878, Length=455, Percent_Identity=23.5164835164835, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787052, Length=458, Percent_Identity=26.4192139737991, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789966, Length=482, Percent_Identity=23.6514522821577, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789887, Length=522, Percent_Identity=23.1800766283525, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787495, Length=466, Percent_Identity=23.3905579399142, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787551, Length=475, Percent_Identity=22.9473684210526, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789397, Length=452, Percent_Identity=25.2212389380531, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54021; Mature: 54021

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIKRMAAAAVFALGLAGPTLAQEGSFTISVTFGPTAEVPDPRAGYNGWMSNQTGVTET
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
LMGIDYDLNLYPRLAENIAQIAPTQWRVTLREGLQFHDGSPVTAQAVIDAIAPISQADHP
HHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHCCEECCCCCCHHHHHHHHHHHCCCCCCC
GYNARIARVLDLAGMSAQDDRTVVFETNSPNAAFQWSLSDPGVAILGAASEAFPINATGP
CCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCH
YVFREAIVDQLYRVEANPDYRLGAPGFEEVRVAASPDPAAAALAFEAGEVDLVINYPETD
HHHHHHHHHHHHEECCCCCCEECCCCHHHEEEECCCCCCCEEEEEECCCEEEEEECCCCH
FERIQETGALGFTAPTARLYFYSMNTASGPLANPLIRRAVSLAIDRDGIVEAVLSGVGGV
HHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC
PAGTIYPAGKSWAADIAPTYDPARAEALLSEAGAVKRGGRWMLDGEPIEIDILTYSSRAA
CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCC
LPPTAEITQAFLQQIGITARVRVGEYGASNDAMLAGEGDMFLQAWVMLPQGDPGSVLGFL
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEECCCCCCCEEEEE
LASDGGANSGRYANPALDELLIKGQTTFEQSERERIYDEVQQIIATDVPLIPVFHVSQAN
EECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
VARAGLTGYAVHPTETYWLTHETALSE
HHHCCCCCEEECCCCEEEEEEHHHCCC
>Mature Secondary Structure
MLLIKRMAAAAVFALGLAGPTLAQEGSFTISVTFGPTAEVPDPRAGYNGWMSNQTGVTET
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
LMGIDYDLNLYPRLAENIAQIAPTQWRVTLREGLQFHDGSPVTAQAVIDAIAPISQADHP
HHCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHCCEECCCCCCHHHHHHHHHHHCCCCCCC
GYNARIARVLDLAGMSAQDDRTVVFETNSPNAAFQWSLSDPGVAILGAASEAFPINATGP
CCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCH
YVFREAIVDQLYRVEANPDYRLGAPGFEEVRVAASPDPAAAALAFEAGEVDLVINYPETD
HHHHHHHHHHHHEECCCCCCEECCCCHHHEEEECCCCCCCEEEEEECCCEEEEEECCCCH
FERIQETGALGFTAPTARLYFYSMNTASGPLANPLIRRAVSLAIDRDGIVEAVLSGVGGV
HHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC
PAGTIYPAGKSWAADIAPTYDPARAEALLSEAGAVKRGGRWMLDGEPIEIDILTYSSRAA
CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCC
LPPTAEITQAFLQQIGITARVRVGEYGASNDAMLAGEGDMFLQAWVMLPQGDPGSVLGFL
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCEEEEEEEEECCCCCCCEEEEE
LASDGGANSGRYANPALDELLIKGQTTFEQSERERIYDEVQQIIATDVPLIPVFHVSQAN
EECCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
VARAGLTGYAVHPTETYWLTHETALSE
HHHCCCCCEEECCCCEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]