Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 110679121

GI number: 110679121

Start: 1750617

End: 1751366

Strand: Reverse

Name: cobS [H]

Synonym: RD1_1826

Alternate gene names: 110679121

Gene position: 1751366-1750617 (Counterclockwise)

Preceding gene: 110679123

Following gene: 110679115

Centisome position: 42.37

GC content: 63.47

Gene sequence:

>750_bases
ATGATCCCGTCTTTCGTGCGCGAGATCGGGGTCGCCTTTTGTCTGCTGTCACGCCTGCCCATGCCCGCTCTGCCGGCCGC
TGCATTTGAGCGAAGTGCGCGCAGTGTCTGGGCTTATCCGCTTGTCGGGGCAACGCTGGGTCTGATCGCAGGGGGCGTCG
GCGCCGCGGCCCTGTGGGCCGGGCTGCCTGTAGGCATCGCCGCCGGTTTGACACTCGCAGCTTTGATGCTGAGCACGGGC
GCCATGCACGAAGATGGCCTCGCCGACACGGCAGACGGTTTTTGGGGCGGGTTTGATCGCGACCGCCGCCTTGAGATCAT
GAAAGACAGCCAGATCGGAACCTATGGCACCCTGTCACTGATCGTGACAAGCGGGCTGCGATGGGCTGCTCTTGCGGTTC
TGCTGCCTGCGGGGATAGCCCCGGTGATCGTCGCCGCTTGTTTGTCACGCGCCGCGATGCCCGTGATCATGCGCCATGTC
CCACATGCGCGTGGCGATGGGCTGTCGCACAGCGTCGGTCGACCGGCAATGCCACATGTGTTCGCGGGCCTGTGCATTGC
GGTCTTGCTGGGTGCGGTTCTGTCAGGGCCTGTCGTCCTTGTCGCGCTGGCGCTTGGTGCGGGCCTTGTTTTCGGGGCCA
TAAAACTCACGCAACACAAAATCGGTGGCCATACAGGAGATGTCCTCGGGGCCACGCAGGTGATCACCGAATTGCTGGTC
TTGCTCACCTTCATTGTGGCCACCCCATGA

Upstream 100 bases:

>100_bases
CCGTCATCAGAGAACCCCGTTGCATGATTGTTGCGTCCTCTTTACCCCAAGGGACGCAACCAGCAAGATGTGAAGAGGCC
GCAGGCGCATGAAAACCGAC

Downstream 100 bases:

>100_bases
AAAAACCGCGCCGGCACGGGCGCGGTTTTCAACAGTAGCCTTGGCTGCGTTCAGGCGGCGACGCGGCTTTCCAGAACGTC
ACCGACCTGTTTGGAGGCGG

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIPSFVREIGVAFCLLSRLPMPALPAAAFERSARSVWAYPLVGATLGLIAGGVGAAALWAGLPVGIAAGLTLAALMLSTG
AMHEDGLADTADGFWGGFDRDRRLEIMKDSQIGTYGTLSLIVTSGLRWAALAVLLPAGIAPVIVAACLSRAAMPVIMRHV
PHARGDGLSHSVGRPAMPHVFAGLCIAVLLGAVLSGPVVLVALALGAGLVFGAIKLTQHKIGGHTGDVLGATQVITELLV
LLTFIVATP

Sequences:

>Translated_249_residues
MIPSFVREIGVAFCLLSRLPMPALPAAAFERSARSVWAYPLVGATLGLIAGGVGAAALWAGLPVGIAAGLTLAALMLSTG
AMHEDGLADTADGFWGGFDRDRRLEIMKDSQIGTYGTLSLIVTSGLRWAALAVLLPAGIAPVIVAACLSRAAMPVIMRHV
PHARGDGLSHSVGRPAMPHVFAGLCIAVLLGAVLSGPVVLVALALGAGLVFGAIKLTQHKIGGHTGDVLGATQVITELLV
LLTFIVATP
>Mature_249_residues
MIPSFVREIGVAFCLLSRLPMPALPAAAFERSARSVWAYPLVGATLGLIAGGVGAAALWAGLPVGIAAGLTLAALMLSTG
AMHEDGLADTADGFWGGFDRDRRLEIMKDSQIGTYGTLSLIVTSGLRWAALAVLLPAGIAPVIVAACLSRAAMPVIMRHV
PHARGDGLSHSVGRPAMPHVFAGLCIAVLLGAVLSGPVVLVALALGAGLVFGAIKLTQHKIGGHTGDVLGATQVITELLV
LLTFIVATP

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes Ado-cobalamin 5'-phosphate from Ado-cobinamide-GDP and alpha-ribazole 5'- phosphate [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=239, Percent_Identity=35.1464435146443, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25390; Mature: 25390

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCC
>Mature Secondary Structure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HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1917841 [H]