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The map label for this gene is rimO

Identifier: 110678963

GI number: 110678963

Start: 1593682

End: 1595061

Strand: Reverse

Name: rimO

Synonym: RD1_1659

Alternate gene names: 110678963

Gene position: 1595061-1593682 (Counterclockwise)

Preceding gene: 110678965

Following gene: 110678962

Centisome position: 38.59

GC content: 61.45

Gene sequence:

>1380_bases
ATGAGCACAAACCCACCAGACCTGCGCCCCGATCTTGCGCCCAAAGCCCGGCTGACGCAACCGGACCGTCCCGGACAGCC
GACAATCGGCATGGTCAGCCTCGGCTGCCCCAAAGCACTTGTCGACAGTGAACGCATTTTGACGCGATTGCGCGCGGAAG
GCTACGGCATATCACCGGATTATGATGGGGCGGATGCGGTCATCGTGAATACCTGCGGATTTCTGGACAGCGCCAAGGCG
GAATCGCTTTCTGCCATCGGGGAAGCGCTAAATGAGAACGGCCGGGTGATCGTCACGGGCTGTCTGGGCGCTGAGCCCGA
GTACATCACAGGGGCGCACCCAAAGGTGCTGGCAGTGACCGGCCCGCAACAATACGAACAGGTGCTGGATGCGGTGCATG
CCGCCGTGCCGCCCAGCCCGGACCCTTTTGTTGATCTCTTGCCTGCAACGGCGGTCAGCCTGACACCGCGCCATTTCAGC
TATCTGAAGATTTCCGAGGGCTGCAATCACAAGTGCAAATTCTGCATCATCCCTGACATGCGCGGGCGTCTGGCCAGCCG
TCCGGCCCATGCGGTGATGCGGGAAGCGGAAAAGCTGGTCGACAACGGCGTCAGGGAACTGCTGGTCATCAGTCAGGACA
CATCGGCCTTTGGCGTGGACATCAAACACGCCGAGGAGCGCGGGCACCGCGCCCACATCACCGACCTTGCACGCGACCTC
GGATCGCTGGGGGCATGGGTTCGGCTGCATTACGTCTATCCCTACCCCCATGTGCGCCAGTTGATCCCGCTGATGGCGGA
CGGTTTGGTGCTGCCCTATCTGGACATCCCCTTTCAGCACGCTCATCCGGATGTGTTGCGCCGCATGGCACGCCCTGCCG
CTGCTGCAAAAACGCTGGATGAAATCGCGGCTTGGCGCGCGATTTGTCCGGACCTGACGCTGCGCTCGACCTTTATCGTG
GGCTATCCCGGCGAAACGGAGGAAGAGTTTCAGACCCTTCTTGACTGGCTGGACGAAGCGCAACTGGACCGGGTCGGGTG
CTTTCAATATGAAAACGTCGCCGGTGCACGCTCAAACGCGCTGCCGGATCATGTGCCCGAGGACGTGAAGCAGGACCGCT
GGAACCGGTTCATGGCCAAGTCGCAGGACATCTCTGAAGCGAAACTCGCCGCGAAAGTCGCTCAGCGCCTTGAGGTGATC
GTGGATGAGGTGGACGCTGATGCCGCCACCTGCCGCACCAAAGCCGATGCGCCGGAAATCGACGGCAACCTGTTCATTGA
TGACGGCCACGATGGCTTGAAACCGGGCGACATCGTCACGGTCGAGGTCGATGAGGCAGGTGAATACGACCTGTGGGGAC
GCCTTGCGCCATCGGCGTAA

Upstream 100 bases:

>100_bases
AAGATCAGCCCGAAAAAACGAAAAACCCATTTCATCCCGCACACTCCCGCGCCAACGCAAGGCCTTTCAAGCCTCTGACA
ACTGCAGTATAGCGCAGATC

Downstream 100 bases:

>100_bases
CCGCGGAGTTTGCCTTTGACGCGGCTGCGCTATCTTTTGCACACAGCAAGGAGAACCCAGCCATGTCCGACACGCCCAAA
ATCGCCCAGACCGCCCCCTA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSTNPPDLRPDLAPKARLTQPDRPGQPTIGMVSLGCPKALVDSERILTRLRAEGYGISPDYDGADAVIVNTCGFLDSAKA
ESLSAIGEALNENGRVIVTGCLGAEPEYITGAHPKVLAVTGPQQYEQVLDAVHAAVPPSPDPFVDLLPATAVSLTPRHFS
YLKISEGCNHKCKFCIIPDMRGRLASRPAHAVMREAEKLVDNGVRELLVISQDTSAFGVDIKHAEERGHRAHITDLARDL
GSLGAWVRLHYVYPYPHVRQLIPLMADGLVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIAAWRAICPDLTLRSTFIV
GYPGETEEEFQTLLDWLDEAQLDRVGCFQYENVAGARSNALPDHVPEDVKQDRWNRFMAKSQDISEAKLAAKVAQRLEVI
VDEVDADAATCRTKADAPEIDGNLFIDDGHDGLKPGDIVTVEVDEAGEYDLWGRLAPSA

Sequences:

>Translated_459_residues
MSTNPPDLRPDLAPKARLTQPDRPGQPTIGMVSLGCPKALVDSERILTRLRAEGYGISPDYDGADAVIVNTCGFLDSAKA
ESLSAIGEALNENGRVIVTGCLGAEPEYITGAHPKVLAVTGPQQYEQVLDAVHAAVPPSPDPFVDLLPATAVSLTPRHFS
YLKISEGCNHKCKFCIIPDMRGRLASRPAHAVMREAEKLVDNGVRELLVISQDTSAFGVDIKHAEERGHRAHITDLARDL
GSLGAWVRLHYVYPYPHVRQLIPLMADGLVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIAAWRAICPDLTLRSTFIV
GYPGETEEEFQTLLDWLDEAQLDRVGCFQYENVAGARSNALPDHVPEDVKQDRWNRFMAKSQDISEAKLAAKVAQRLEVI
VDEVDADAATCRTKADAPEIDGNLFIDDGHDGLKPGDIVTVEVDEAGEYDLWGRLAPSA
>Mature_458_residues
STNPPDLRPDLAPKARLTQPDRPGQPTIGMVSLGCPKALVDSERILTRLRAEGYGISPDYDGADAVIVNTCGFLDSAKAE
SLSAIGEALNENGRVIVTGCLGAEPEYITGAHPKVLAVTGPQQYEQVLDAVHAAVPPSPDPFVDLLPATAVSLTPRHFSY
LKISEGCNHKCKFCIIPDMRGRLASRPAHAVMREAEKLVDNGVRELLVISQDTSAFGVDIKHAEERGHRAHITDLARDLG
SLGAWVRLHYVYPYPHVRQLIPLMADGLVLPYLDIPFQHAHPDVLRRMARPAAAAKTLDEIAAWRAICPDLTLRSTFIVG
YPGETEEEFQTLLDWLDEAQLDRVGCFQYENVAGARSNALPDHVPEDVKQDRWNRFMAKSQDISEAKLAAKVAQRLEVIV
DEVDADAATCRTKADAPEIDGNLFIDDGHDGLKPGDIVTVEVDEAGEYDLWGRLAPSA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=493, Percent_Identity=23.7322515212982, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28872784, Length=486, Percent_Identity=24.6913580246914, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28872782, Length=486, Percent_Identity=24.6913580246914, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=62.5866050808314, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786882, Length=358, Percent_Identity=25.9776536312849, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=24.4725738396624, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=203, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356207, Length=471, Percent_Identity=22.7176220806794, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ROSDO (Q169Q9)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681970.1
- ProteinModelPortal:   Q169Q9
- SMR:   Q169Q9
- STRING:   Q169Q9
- GeneID:   4197073
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1659
- NMPDR:   fig|375451.6.peg.1553
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q169Q9
- ProtClustDB:   PRK14862
- BioCyc:   RDEN375451:RD1_1659-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50149; Mature: 50018

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNPPDLRPDLAPKARLTQPDRPGQPTIGMVSLGCPKALVDSERILTRLRAEGYGISPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
YDGADAVIVNTCGFLDSAKAESLSAIGEALNENGRVIVTGCLGAEPEYITGAHPKVLAVT
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
GPQQYEQVLDAVHAAVPPSPDPFVDLLPATAVSLTPRHFSYLKISEGCNHKCKFCIIPDM
CHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHEECCCCCEEEEECCCCCCCEEEEECCCH
RGRLASRPAHAVMREAEKLVDNGVRELLVISQDTSAFGVDIKHAEERGHRAHITDLARDL
HHHHHCCHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCEEHHHHHCCCCHHHHHHHHHH
GSLGAWVRLHYVYPYPHVRQLIPLMADGLVLPYLDIPFQHAHPDVLRRMARPAAAAKTLD
HHHCCEEEEEEECCCHHHHHHHHHHHCCEEECEECCCCCCCCHHHHHHHHCHHHHHHHHH
EIAAWRAICPDLTLRSTFIVGYPGETEEEFQTLLDWLDEAQLDRVGCFQYENVAGARSNA
HHHHHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCC
LPDHVPEDVKQDRWNRFMAKSQDISEAKLAAKVAQRLEVIVDEVDADAATCRTKADAPEI
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
DGNLFIDDGHDGLKPGDIVTVEVDEAGEYDLWGRLAPSA
CCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STNPPDLRPDLAPKARLTQPDRPGQPTIGMVSLGCPKALVDSERILTRLRAEGYGISPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
YDGADAVIVNTCGFLDSAKAESLSAIGEALNENGRVIVTGCLGAEPEYITGAHPKVLAVT
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
GPQQYEQVLDAVHAAVPPSPDPFVDLLPATAVSLTPRHFSYLKISEGCNHKCKFCIIPDM
CHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHEECCCCCEEEEECCCCCCCEEEEECCCH
RGRLASRPAHAVMREAEKLVDNGVRELLVISQDTSAFGVDIKHAEERGHRAHITDLARDL
HHHHHCCHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCEEHHHHHCCCCHHHHHHHHHH
GSLGAWVRLHYVYPYPHVRQLIPLMADGLVLPYLDIPFQHAHPDVLRRMARPAAAAKTLD
HHHCCEEEEEEECCCHHHHHHHHHHHCCEEECEECCCCCCCCHHHHHHHHCHHHHHHHHH
EIAAWRAICPDLTLRSTFIVGYPGETEEEFQTLLDWLDEAQLDRVGCFQYENVAGARSNA
HHHHHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCC
LPDHVPEDVKQDRWNRFMAKSQDISEAKLAAKVAQRLEVIVDEVDADAATCRTKADAPEI
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
DGNLFIDDGHDGLKPGDIVTVEVDEAGEYDLWGRLAPSA
CCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA