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The map label for this gene is braC [H]

Identifier: 110678814

GI number: 110678814

Start: 1427247

End: 1428398

Strand: Direct

Name: braC [H]

Synonym: RD1_1502

Alternate gene names: 110678814

Gene position: 1427247-1428398 (Clockwise)

Preceding gene: 110678813

Following gene: 110678815

Centisome position: 34.53

GC content: 58.94

Gene sequence:

>1152_bases
ATGAAAAAGTCCATAATCACCGCGCTGGCATGCACCGCATTGGTGACACCCGCCTTTGCCCAAGACACCATCAAGATTGG
CCTCGGCGTGCCGATGACCGGTGACTATGCGCCCTATTCCGAATGGCAGGGGGCCCGCTGCATGGCTGAAATGATCAACG
CAGATGGTGGCGTTGACGGCAAGATGATCGAGATTCTGGTGCAGGATTCCGGTGCTGACACGCAGACCGCGATCTCTCTG
GCGCAGAAGTTTCTGGACGAAGGGGCGGTCATGCTGGGCACGATCCCGTTTTCCGACACGATGATACCTGTCGCCCAGAT
CGCGCAGGGCTATGGCGTGTCGATCTTTCAGCCACAATCGACTCAGGTCGAGATGCACGCAGGCATCGTCGATAACTTCT
TTACCGGTGTTTCACCTGATCCTTTCACCGCGACAGCAGCCGCCAACTATGCGCTGGAAAAAGGCGTCAAGAACGTCGTC
CTGCTGGCATCTGACGAAGGGGGCAGCTGGTCTGCCAAAACGCCGCTTTGGTTCGGCGAGGTCATCGAAGCAGGTGGCGG
CAAGGTCCTGAGCACGCTGAATTTCAGCTTTGGCACATCTGACTGGTCCCCCCAGATCGCGGAAATGCGCGCACTGGGGG
AAGAGGTGGACGCGGTCTATATCTCTTCGATCATGCCGGATGTCGGCGTCTTGGTGCGCCAGTTGCGCAGTGCTGGCATA
GATGCCTGGGTCGTGGGCTCAGATGGTTTTGATGACCCGTCGCTGGACGCCATCGGCACGGATGATCCGTCGATCCTTGA
CAAGGTCTTCTTTGCCACGCTCGCGCCAAGCCAGGCGGACAGCGCGGTCGTCCAGTTCATGGCTGATTGCAAAGAGATGG
GCATCGATGTGCCGGGTCTTTTCCCGGCGACGGGCGCGGATACGGTCAAGGCGCTGGCCTGGGCGGTGGAGCAATCGGGC
AGCGTGGATCCTGAGACCATCGCCCAAACGATCCGCGACGCGGACAGTATTCCGGTGCTGACCGTGGATAGCATTTCGTT
CAAGGAGACCAGGACATACGCGCAGCGCACCATTCCCGTCATCGGGTTTAAAGATGGCAAGCGCGTGCTGATTTCGAACG
AAATCCCTGAAAACGTGCCGGCCGACTGGTAA

Upstream 100 bases:

>100_bases
CGAAGAGGTCACCAAGAACCCAAAGGTTGTCACCGCCTACCTCGGAGAAGACGCAGGCACTGACGCCTGAGTCCAAGAAA
CCAACATCAAGGAGAACGTA

Downstream 100 bases:

>100_bases
AGGCAAAGGGGGGCAGGGCTTTATCACCATGCCCCCGTGACCTGATGTTGGATATTCAGAACCTCCGCGTTTCGCATGGC
CCCATCGAGGCGGTGCATGG

Product: branched-chain amino acid ABC transporter, periplasmic binding protein, putative

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKKSIITALACTALVTPAFAQDTIKIGLGVPMTGDYAPYSEWQGARCMAEMINADGGVDGKMIEILVQDSGADTQTAISL
AQKFLDEGAVMLGTIPFSDTMIPVAQIAQGYGVSIFQPQSTQVEMHAGIVDNFFTGVSPDPFTATAAANYALEKGVKNVV
LLASDEGGSWSAKTPLWFGEVIEAGGGKVLSTLNFSFGTSDWSPQIAEMRALGEEVDAVYISSIMPDVGVLVRQLRSAGI
DAWVVGSDGFDDPSLDAIGTDDPSILDKVFFATLAPSQADSAVVQFMADCKEMGIDVPGLFPATGADTVKALAWAVEQSG
SVDPETIAQTIRDADSIPVLTVDSISFKETRTYAQRTIPVIGFKDGKRVLISNEIPENVPADW

Sequences:

>Translated_383_residues
MKKSIITALACTALVTPAFAQDTIKIGLGVPMTGDYAPYSEWQGARCMAEMINADGGVDGKMIEILVQDSGADTQTAISL
AQKFLDEGAVMLGTIPFSDTMIPVAQIAQGYGVSIFQPQSTQVEMHAGIVDNFFTGVSPDPFTATAAANYALEKGVKNVV
LLASDEGGSWSAKTPLWFGEVIEAGGGKVLSTLNFSFGTSDWSPQIAEMRALGEEVDAVYISSIMPDVGVLVRQLRSAGI
DAWVVGSDGFDDPSLDAIGTDDPSILDKVFFATLAPSQADSAVVQFMADCKEMGIDVPGLFPATGADTVKALAWAVEQSG
SVDPETIAQTIRDADSIPVLTVDSISFKETRTYAQRTIPVIGFKDGKRVLISNEIPENVPADW
>Mature_383_residues
MKKSIITALACTALVTPAFAQDTIKIGLGVPMTGDYAPYSEWQGARCMAEMINADGGVDGKMIEILVQDSGADTQTAISL
AQKFLDEGAVMLGTIPFSDTMIPVAQIAQGYGVSIFQPQSTQVEMHAGIVDNFFTGVSPDPFTATAAANYALEKGVKNVV
LLASDEGGSWSAKTPLWFGEVIEAGGGKVLSTLNFSFGTSDWSPQIAEMRALGEEVDAVYISSIMPDVGVLVRQLRSAGI
DAWVVGSDGFDDPSLDAIGTDDPSILDKVFFATLAPSQADSAVVQFMADCKEMGIDVPGLFPATGADTVKALAWAVEQSG
SVDPETIAQTIRDADSIPVLTVDSISFKETRTYAQRTIPVIGFKDGKRVLISNEIPENVPADW

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=335, Percent_Identity=24.7761194029851, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994941, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40495; Mature: 40495

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIITALACTALVTPAFAQDTIKIGLGVPMTGDYAPYSEWQGARCMAEMINADGGVDG
CCCHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
KMIEILVQDSGADTQTAISLAQKFLDEGAVMLGTIPFSDTMIPVAQIAQGYGVSIFQPQS
EEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCEEEECCCC
TQVEMHAGIVDNFFTGVSPDPFTATAAANYALEKGVKNVVLLASDEGGSWSAKTPLWFGE
CCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
VIEAGGGKVLSTLNFSFGTSDWSPQIAEMRALGEEVDAVYISSIMPDVGVLVRQLRSAGI
HHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DAWVVGSDGFDDPSLDAIGTDDPSILDKVFFATLAPSQADSAVVQFMADCKEMGIDVPGL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
FPATGADTVKALAWAVEQSGSVDPETIAQTIRDADSIPVLTVDSISFKETRTYAQRTIPV
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCE
IGFKDGKRVLISNEIPENVPADW
EEECCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKSIITALACTALVTPAFAQDTIKIGLGVPMTGDYAPYSEWQGARCMAEMINADGGVDG
CCCHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
KMIEILVQDSGADTQTAISLAQKFLDEGAVMLGTIPFSDTMIPVAQIAQGYGVSIFQPQS
EEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCEEEECCCC
TQVEMHAGIVDNFFTGVSPDPFTATAAANYALEKGVKNVVLLASDEGGSWSAKTPLWFGE
CCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHH
VIEAGGGKVLSTLNFSFGTSDWSPQIAEMRALGEEVDAVYISSIMPDVGVLVRQLRSAGI
HHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DAWVVGSDGFDDPSLDAIGTDDPSILDKVFFATLAPSQADSAVVQFMADCKEMGIDVPGL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
FPATGADTVKALAWAVEQSGSVDPETIAQTIRDADSIPVLTVDSISFKETRTYAQRTIPV
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCE
IGFKDGKRVLISNEIPENVPADW
EEECCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]