Click here to switch to the map view.

The map label for this gene is mtlK [H]

Identifier: 110678793

GI number: 110678793

Start: 1405379

End: 1406860

Strand: Reverse

Name: mtlK [H]

Synonym: RD1_1481

Alternate gene names: 110678793

Gene position: 1406860-1405379 (Counterclockwise)

Preceding gene: 110678794

Following gene: 110678792

Centisome position: 34.04

GC content: 58.23

Gene sequence:

>1482_bases
ATGGATGAACTGATCGCCCTTTCAAATGCGACGCTGGATCGACTGCGCGTTGAACGTCCAAACTATGATCGCGGGGCCCT
GACACCGGGCATTGTTCACATCGGTGTCGGTAATTTCCATCGTGCGCATCAGGCCTGGTATCTGCACCGTCTGATGCAGG
ACGGCAAAGCGCATGACTGGGCCATCATCGGAGCGGGGGTGCGCCCCTACGATACCGCGATGCGCGAAAAACTGATGGCG
CAGGACTGCCTTACCACGCTGATCGAACTGGACCCTCGGAAAACCTCGGCTGAGGTGGTGGGTTCGATGATCGACTACCT
GCCGATCGAGGATGGAAATGGCGCGCTGATCCGTCAGATGGCGGACCCTTCCATCCGGATCGTCTCGATGACCGTTACCG
AAAGCGGGTACTATATCGACCCGGTCACCAAGGGGTTTGATGCCAGCCACGAAGACATGCTTCACGATGCTGCACATGTC
GATACGCCGCGCACGGCGTTTGGCGCAATCATCGCCGCACTTCGCGCCCGTCGTGCTGCCGGGCATCTGCCTTTTACCTG
CCAAAGCTGTGACAATCTGCAGGGCAATGGCGATATTCTGCGACAGACGGTGGTATCGCTCGCTCGCATGTCCGATCCTG
CTCTTGCCGGTTGGATCGATGAAAATGGGGCCTTCCCCAATTCGATGGTTGATTGCATTGCGCCTGCCACCGGTCCCAAG
GAGCTCGCCCTTGCCCGCGATTTCGGGATCAAGGACAACGCAGTAGTGACGCATGAAGCCTTCCGTCAATGGGTCATCGA
AGACGCATTTTGCGCAGGCCGTCCGGATTGGGAACAAACCGGCGCGACATTCACGGATGATGTCCACGCATATGAGACGA
TGAAAATCCGCATTCTGAACGCGGGCCATCAGGTCATTGCAAATGTTGGTGAAACCCTCGGGATTGAGACGATTGCAGGC
TGTATGGCCGATCCCAACATTCGCGCCTTCTTCGGCGCGGTCCAGAGAAGCGAGATTGCGCCGACTGTCGCGCCGGTGCC
GGGAAAGACACCGGCAGAATACGTTGACCTGATCGAGCAGCGGTTCGCGAACCCGCAGATTGTCGACACGACACGTCGCG
TGGCCTTTGACGGCTCCGCGCGTCACACCGGATTCGTGCTGCCCATTCTGCGCGATCAACTGGCTGCAGGGCGATCCATT
GAAGGGCTCGCGCTGGTTGAGGCCCTCTGGGCGCGCATGTGTGCAGGTACGCGCGAAGATGGCTCTGAGATCGCCGCAAA
CGATCCTATCTGGGATGCGCTCAAAGATGCTGCCTTGGCTGCAAAAACACGCCCCGTGGCATGGCTTGAGCAGACCAATC
TATACGGGGATCTATTGGAAAACCCCACGTTCTCTCGGGCATTTATCAGGTGGTTGGAGATGATCTGGTCGCAAGGCTGC
CAGGCTGCTCTTGTCGAATATACCAAGAGTGCGCGCGCATAA

Upstream 100 bases:

>100_bases
GCGGCGGCACCATGGCTTACCTGACCCAAACGGATGTCTGTCCGGCGGGCCGCGCCGCAATCCCTATCATCGCACAACCT
TTTAACGTGGATATTGGACC

Downstream 100 bases:

>100_bases
AGCGTCTGACCAAATACCTCACACATCGAATATCACGTAACGCGTTGAAATCTTTGATTTCAGGCAGCGTTATGTGATCG
AGGTTTTCCGCATGACGCTT

Product: mannitol 2-dehydrogenase

Products: NA

Alternate protein names: M2DH; MDH [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MDELIALSNATLDRLRVERPNYDRGALTPGIVHIGVGNFHRAHQAWYLHRLMQDGKAHDWAIIGAGVRPYDTAMREKLMA
QDCLTTLIELDPRKTSAEVVGSMIDYLPIEDGNGALIRQMADPSIRIVSMTVTESGYYIDPVTKGFDASHEDMLHDAAHV
DTPRTAFGAIIAALRARRAAGHLPFTCQSCDNLQGNGDILRQTVVSLARMSDPALAGWIDENGAFPNSMVDCIAPATGPK
ELALARDFGIKDNAVVTHEAFRQWVIEDAFCAGRPDWEQTGATFTDDVHAYETMKIRILNAGHQVIANVGETLGIETIAG
CMADPNIRAFFGAVQRSEIAPTVAPVPGKTPAEYVDLIEQRFANPQIVDTTRRVAFDGSARHTGFVLPILRDQLAAGRSI
EGLALVEALWARMCAGTREDGSEIAANDPIWDALKDAALAAKTRPVAWLEQTNLYGDLLENPTFSRAFIRWLEMIWSQGC
QAALVEYTKSARA

Sequences:

>Translated_493_residues
MDELIALSNATLDRLRVERPNYDRGALTPGIVHIGVGNFHRAHQAWYLHRLMQDGKAHDWAIIGAGVRPYDTAMREKLMA
QDCLTTLIELDPRKTSAEVVGSMIDYLPIEDGNGALIRQMADPSIRIVSMTVTESGYYIDPVTKGFDASHEDMLHDAAHV
DTPRTAFGAIIAALRARRAAGHLPFTCQSCDNLQGNGDILRQTVVSLARMSDPALAGWIDENGAFPNSMVDCIAPATGPK
ELALARDFGIKDNAVVTHEAFRQWVIEDAFCAGRPDWEQTGATFTDDVHAYETMKIRILNAGHQVIANVGETLGIETIAG
CMADPNIRAFFGAVQRSEIAPTVAPVPGKTPAEYVDLIEQRFANPQIVDTTRRVAFDGSARHTGFVLPILRDQLAAGRSI
EGLALVEALWARMCAGTREDGSEIAANDPIWDALKDAALAAKTRPVAWLEQTNLYGDLLENPTFSRAFIRWLEMIWSQGC
QAALVEYTKSARA
>Mature_493_residues
MDELIALSNATLDRLRVERPNYDRGALTPGIVHIGVGNFHRAHQAWYLHRLMQDGKAHDWAIIGAGVRPYDTAMREKLMA
QDCLTTLIELDPRKTSAEVVGSMIDYLPIEDGNGALIRQMADPSIRIVSMTVTESGYYIDPVTKGFDASHEDMLHDAAHV
DTPRTAFGAIIAALRARRAAGHLPFTCQSCDNLQGNGDILRQTVVSLARMSDPALAGWIDENGAFPNSMVDCIAPATGPK
ELALARDFGIKDNAVVTHEAFRQWVIEDAFCAGRPDWEQTGATFTDDVHAYETMKIRILNAGHQVIANVGETLGIETIAG
CMADPNIRAFFGAVQRSEIAPTVAPVPGKTPAEYVDLIEQRFANPQIVDTTRRVAFDGSARHTGFVLPILRDQLAAGRSI
EGLALVEALWARMCAGTREDGSEIAANDPIWDALKDAALAAKTRPVAWLEQTNLYGDLLENPTFSRAFIRWLEMIWSQGC
QAALVEYTKSARA

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=479, Percent_Identity=37.9958246346555, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1788497, Length=479, Percent_Identity=35.0730688935282, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1787823, Length=475, Percent_Identity=35.7894736842105, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI48994885, Length=390, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790028, Length=237, Percent_Identity=25.7383966244726, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324401, Length=474, Percent_Identity=37.5527426160338, Blast_Score=319, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6320765, Length=474, Percent_Identity=37.5527426160338, Blast_Score=319, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.67 [H]

Molecular weight: Translated: 53992; Mature: 53992

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELIALSNATLDRLRVERPNYDRGALTPGIVHIGVGNFHRAHQAWYLHRLMQDGKAHDW
CCCEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCE
AIIGAGVRPYDTAMREKLMAQDCLTTLIELDPRKTSAEVVGSMIDYLPIEDGNGALIRQM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHCC
ADPSIRIVSMTVTESGYYIDPVTKGFDASHEDMLHDAAHVDTPRTAFGAIIAALRARRAA
CCCCEEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GHLPFTCQSCDNLQGNGDILRQTVVSLARMSDPALAGWIDENGAFPNSMVDCIAPATGPK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCHH
ELALARDFGIKDNAVVTHEAFRQWVIEDAFCAGRPDWEQTGATFTDDVHAYETMKIRILN
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHEEEEEEEEEC
AGHQVIANVGETLGIETIAGCMADPNIRAFFGAVQRSEIAPTVAPVPGKTPAEYVDLIEQ
CCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RFANPQIVDTTRRVAFDGSARHTGFVLPILRDQLAAGRSIEGLALVEALWARMCAGTRED
HCCCCCEECCHHHEEECCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
GSEIAANDPIWDALKDAALAAKTRPVAWLEQTNLYGDLLENPTFSRAFIRWLEMIWSQGC
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCC
QAALVEYTKSARA
HHHHHHHHHHCCC
>Mature Secondary Structure
MDELIALSNATLDRLRVERPNYDRGALTPGIVHIGVGNFHRAHQAWYLHRLMQDGKAHDW
CCCEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCE
AIIGAGVRPYDTAMREKLMAQDCLTTLIELDPRKTSAEVVGSMIDYLPIEDGNGALIRQM
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHCC
ADPSIRIVSMTVTESGYYIDPVTKGFDASHEDMLHDAAHVDTPRTAFGAIIAALRARRAA
CCCCEEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GHLPFTCQSCDNLQGNGDILRQTVVSLARMSDPALAGWIDENGAFPNSMVDCIAPATGPK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCHH
ELALARDFGIKDNAVVTHEAFRQWVIEDAFCAGRPDWEQTGATFTDDVHAYETMKIRILN
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHEEEEEEEEEC
AGHQVIANVGETLGIETIAGCMADPNIRAFFGAVQRSEIAPTVAPVPGKTPAEYVDLIEQ
CCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RFANPQIVDTTRRVAFDGSARHTGFVLPILRDQLAAGRSIEGLALVEALWARMCAGTRED
HCCCCCEECCHHHEEECCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
GSEIAANDPIWDALKDAALAAKTRPVAWLEQTNLYGDLLENPTFSRAFIRWLEMIWSQGC
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCC
QAALVEYTKSARA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8254318 [H]