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The map label for this gene is pdxJ

Identifier: 110678682

GI number: 110678682

Start: 1310708

End: 1311457

Strand: Direct

Name: pdxJ

Synonym: RD1_1362

Alternate gene names: 110678682

Gene position: 1310708-1311457 (Clockwise)

Preceding gene: 110678681

Following gene: 110678683

Centisome position: 31.71

GC content: 61.6

Gene sequence:

>750_bases
ATGTCCGATCAAAAAAAACTGCGTCTTGGCGTGAATATCGACCATGTGGCGACGGTGCGCAATGCGCGCGGGGGGGCCTA
TCCTGATCCGCTGCGCGCGGCCCGCATTGCCGAAGAGGCTGGCGCGGATGGCATCACGGCGCATCTGCGCGAAGACCGCC
GTCACATTTCGGATGCGGATATCGAAGGGTTGATGGATGTGCTGAGCGTGCCGCTCAATTTCGAGATGGCAGCGACGGAT
GAGATGCAACAGATCGCCCTGCGCCATAAACCGCACGCGGTGTGCATTGTGCCCGAAAAGCGCGAAGAGCGCACGACCGA
AGGCGGTCTTGAGGTCGCCCGCGAGGAAAACGTGCTGGCCCATTTCATTGCGCCGCTGCGCGAGGCCGGGTGCCGGGTGT
CGATCTTTATCGCTGCAGAGCAACGTCAGATCGAAGCGGCGCACCGGATCGGGGCCGAGGTCATCGAACTGCACACCGGT
GCCTATTGCGACGCCCATGCCGAAGGGGATTTCGCACAGCGCGATGCGGAACTGGCGCGTCTGAGGGATATGGCCACCTT
TGCGCATGGGTTGGGCCTTGAGGTCCACGCAGGCCACGGGCTGACCTATGACACCGTGCAGCCCATTGCCGCTTTTCCCG
AAGTGATCGAACTCAACATCGGACATTTCCTGATTGGTGAAAGCATCTTTCGTGGGCTGGAACCCGCCATCGCAGAAATG
CGCCGCCTGATGGATGCGGCGCGGGCATGA

Upstream 100 bases:

>100_bases
GATCAAGGCCAAAGCGGCCAGCAAAGCAGAGGCGAAACGAAAAGCGGAGCGTCCGCCCGCCGGGGAGTGACCCGGCCACA
ATACTAGGGGAGGGCTTGAG

Downstream 100 bases:

>100_bases
CAGACGCAATGTTTTTGTTGCCTTTGCCGCTTATCCTGTTGGGATGGGTCATCGGCGGGGGAAGCCCCGGGCCGGCGACA
CTCGCAATCTCGGGCACTTC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSDQKKLRLGVNIDHVATVRNARGGAYPDPLRAARIAEEAGADGITAHLREDRRHISDADIEGLMDVLSVPLNFEMAATD
EMQQIALRHKPHAVCIVPEKREERTTEGGLEVAREENVLAHFIAPLREAGCRVSIFIAAEQRQIEAAHRIGAEVIELHTG
AYCDAHAEGDFAQRDAELARLRDMATFAHGLGLEVHAGHGLTYDTVQPIAAFPEVIELNIGHFLIGESIFRGLEPAIAEM
RRLMDAARA

Sequences:

>Translated_249_residues
MSDQKKLRLGVNIDHVATVRNARGGAYPDPLRAARIAEEAGADGITAHLREDRRHISDADIEGLMDVLSVPLNFEMAATD
EMQQIALRHKPHAVCIVPEKREERTTEGGLEVAREENVLAHFIAPLREAGCRVSIFIAAEQRQIEAAHRIGAEVIELHTG
AYCDAHAEGDFAQRDAELARLRDMATFAHGLGLEVHAGHGLTYDTVQPIAAFPEVIELNIGHFLIGESIFRGLEPAIAEM
RRLMDAARA
>Mature_248_residues
SDQKKLRLGVNIDHVATVRNARGGAYPDPLRAARIAEEAGADGITAHLREDRRHISDADIEGLMDVLSVPLNFEMAATDE
MQQIALRHKPHAVCIVPEKREERTTEGGLEVAREENVLAHFIAPLREAGCRVSIFIAAEQRQIEAAHRIGAEVIELHTGA
YCDAHAEGDFAQRDAELARLRDMATFAHGLGLEVHAGHGLTYDTVQPIAAFPEVIELNIGHFLIGESIFRGLEPAIAEMR
RLMDAARA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=243, Percent_Identity=57.6131687242798, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_ROSDO (Q16AJ0)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681689.1
- ProteinModelPortal:   Q16AJ0
- SMR:   Q16AJ0
- STRING:   Q16AJ0
- GeneID:   4197335
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1362
- NMPDR:   fig|375451.6.peg.1275
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q16AJ0
- ProtClustDB:   PRK05265
- BioCyc:   RDEN375451:RD1_1362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27341; Mature: 27210

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 199-199 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQKKLRLGVNIDHVATVRNARGGAYPDPLRAARIAEEAGADGITAHLREDRRHISDAD
CCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
IEGLMDVLSVPLNFEMAATDEMQQIALRHKPHAVCIVPEKREERTTEGGLEVAREENVLA
HHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHH
HFIAPLREAGCRVSIFIAAEQRQIEAAHRIGAEVIELHTGAYCDAHAEGDFAQRDAELAR
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LRDMATFAHGLGLEVHAGHGLTYDTVQPIAAFPEVIELNIGHFLIGESIFRGLEPAIAEM
HHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHCCCHHHHHH
RRLMDAARA
HHHHHHHCC
>Mature Secondary Structure 
SDQKKLRLGVNIDHVATVRNARGGAYPDPLRAARIAEEAGADGITAHLREDRRHISDAD
CCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
IEGLMDVLSVPLNFEMAATDEMQQIALRHKPHAVCIVPEKREERTTEGGLEVAREENVLA
HHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHH
HFIAPLREAGCRVSIFIAAEQRQIEAAHRIGAEVIELHTGAYCDAHAEGDFAQRDAELAR
HHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LRDMATFAHGLGLEVHAGHGLTYDTVQPIAAFPEVIELNIGHFLIGESIFRGLEPAIAEM
HHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHCCCHHHHHH
RRLMDAARA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA