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The map label for this gene is ispH

Identifier: 110678676

GI number: 110678676

Start: 1304987

End: 1305937

Strand: Reverse

Name: ispH

Synonym: RD1_1355

Alternate gene names: 110678676

Gene position: 1305937-1304987 (Counterclockwise)

Preceding gene: 110678677

Following gene: 110678675

Centisome position: 31.6

GC content: 60.36

Gene sequence:

>951_bases
ATGACGAAACCGCCCCTGACCCTCTATCTTGCAGCGCCCCGTGGCTTTTGCGCGGGCGTTGATCGCGCGATCAAGATCGT
GGAACTGGCGCTCGATAAATGGGGCGCGCCCGTTTATGTCAGGCATGAAATCGTGCACAACAAATTCGTGGTGGACGGGC
TGCGCGCAAAAGGCGCCATCTTTGTCGAAGAGCTTTCGGAATGCCCCGACGACCGACCCGTCATTTTTTCCGCCCATGGC
GTGCCCAAATCCGTGCCCACGGCGGCGCAAGCGCGCAACCTCGTCTATGTGGATGCGACCTGCCCGCTTGTGAGCAAGGT
GCATATCGAAGCCCAACGCCATGCGGACAACGGGCTGCAGATGATCATGATCGGTCATGCAGGCCACCCCGAAACTGTCG
GCACCATGGGGCAATTGCCCGAAGGTGAGGTGCTGTTGGTGGAAACCCCGGAAGATGTGGCGACCGTAAAGGTAAGAGAC
CCCGCGAAACTGGCCTATGTCACACAAACCACGCTCAGCGTTGATGACACCGCCGATGTGGTCCACGCCTTGCAGCAGCG
TTTCCCATTGATCGTGGGCCCGCACAAGGAAGACATCTGCTATGCGACCACCAACCGACAGGAGGCCGTCAAGGCCATGG
CCCCGAAATGTGACGCGATGCTGGTTGTCGGGGCTCCCAACTCGTCAAACTCGCGCCGCCTTGTCGAGGTTGGCGCACGG
GCCGGTTGTCAATACGCGCAACTCGTCCAGCGCGCCACGGATATCGACTGGCGCGCCCTTGATGGCATCAGCAGCATTGG
CATCACCGCCGGGGCATCTGCGCCAGAGGTCTTGATCAACGAGGTGATCGCCGCGTTCGACGCACATTATGATGTGACCC
AAGAGGTGGTCGAGACGGCGGTCGAAAACGTGGAATTCAAAGTACCGCGTGTCCTGCGCGAACCGGCCTGA

Upstream 100 bases:

>100_bases
AGAAAGCCGTTTCAGAGGGGGGCCTGCGTTGCGGCATGTCACCCTCTGGACGCAGCCGCGTCAAACCCTTACCTCTGAGA
CATCTGATCCGGAGTAAGAC

Downstream 100 bases:

>100_bases
CCGTCACCGCATGTCCGCCAGACAAGACACTTTTGGCTCCCCCGATCAGCAAGCGCTTTTGGGCCGGGGGCGGGCCCTGC
ATGATCTGACCAGAAGCAAC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTKPPLTLYLAAPRGFCAGVDRAIKIVELALDKWGAPVYVRHEIVHNKFVVDGLRAKGAIFVEELSECPDDRPVIFSAHG
VPKSVPTAAQARNLVYVDATCPLVSKVHIEAQRHADNGLQMIMIGHAGHPETVGTMGQLPEGEVLLVETPEDVATVKVRD
PAKLAYVTQTTLSVDDTADVVHALQQRFPLIVGPHKEDICYATTNRQEAVKAMAPKCDAMLVVGAPNSSNSRRLVEVGAR
AGCQYAQLVQRATDIDWRALDGISSIGITAGASAPEVLINEVIAAFDAHYDVTQEVVETAVENVEFKVPRVLREPA

Sequences:

>Translated_316_residues
MTKPPLTLYLAAPRGFCAGVDRAIKIVELALDKWGAPVYVRHEIVHNKFVVDGLRAKGAIFVEELSECPDDRPVIFSAHG
VPKSVPTAAQARNLVYVDATCPLVSKVHIEAQRHADNGLQMIMIGHAGHPETVGTMGQLPEGEVLLVETPEDVATVKVRD
PAKLAYVTQTTLSVDDTADVVHALQQRFPLIVGPHKEDICYATTNRQEAVKAMAPKCDAMLVVGAPNSSNSRRLVEVGAR
AGCQYAQLVQRATDIDWRALDGISSIGITAGASAPEVLINEVIAAFDAHYDVTQEVVETAVENVEFKVPRVLREPA
>Mature_315_residues
TKPPLTLYLAAPRGFCAGVDRAIKIVELALDKWGAPVYVRHEIVHNKFVVDGLRAKGAIFVEELSECPDDRPVIFSAHGV
PKSVPTAAQARNLVYVDATCPLVSKVHIEAQRHADNGLQMIMIGHAGHPETVGTMGQLPEGEVLLVETPEDVATVKVRDP
AKLAYVTQTTLSVDDTADVVHALQQRFPLIVGPHKEDICYATTNRQEAVKAMAPKCDAMLVVGAPNSSNSRRLVEVGARA
GCQYAQLVQRATDIDWRALDGISSIGITAGASAPEVLINEVIAAFDAHYDVTQEVVETAVENVEFKVPRVLREPA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=54.5161290322581, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_ROSDO (Q16AJ6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681683.1
- STRING:   Q16AJ6
- GeneID:   4197329
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1355
- NMPDR:   fig|375451.6.peg.1269
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- PhylomeDB:   Q16AJ6
- ProtClustDB:   PRK01045
- BioCyc:   RDEN375451:RD1_1355-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34135; Mature: 34004

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPPLTLYLAAPRGFCAGVDRAIKIVELALDKWGAPVYVRHEIVHNKFVVDGLRAKGAI
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCCCCEE
FVEELSECPDDRPVIFSAHGVPKSVPTAAQARNLVYVDATCPLVSKVHIEAQRHADNGLQ
EHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCCEEEEECCCCCCHHEEEEEHHCCCCCEE
MIMIGHAGHPETVGTMGQLPEGEVLLVETPEDVATVKVRDPAKLAYVTQTTLSVDDTADV
EEEEECCCCCCHHCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEEEEECCCHHHHH
VHALQQRFPLIVGPHKEDICYATTNRQEAVKAMAPKCDAMLVVGAPNSSNSRRLVEVGAR
HHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCC
AGCQYAQLVQRATDIDWRALDGISSIGITAGASAPEVLINEVIAAFDAHYDVTQEVVETA
CCCHHHHHHHHHCCCCHHHHCCCHHCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
VENVEFKVPRVLREPA
HHCCEEECCHHHCCCC
>Mature Secondary Structure 
TKPPLTLYLAAPRGFCAGVDRAIKIVELALDKWGAPVYVRHEIVHNKFVVDGLRAKGAI
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCCCCEE
FVEELSECPDDRPVIFSAHGVPKSVPTAAQARNLVYVDATCPLVSKVHIEAQRHADNGLQ
EHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCCEEEEECCCCCCHHEEEEEHHCCCCCEE
MIMIGHAGHPETVGTMGQLPEGEVLLVETPEDVATVKVRDPAKLAYVTQTTLSVDDTADV
EEEEECCCCCCHHCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEEEEEECCCHHHHH
VHALQQRFPLIVGPHKEDICYATTNRQEAVKAMAPKCDAMLVVGAPNSSNSRRLVEVGAR
HHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCC
AGCQYAQLVQRATDIDWRALDGISSIGITAGASAPEVLINEVIAAFDAHYDVTQEVVETA
CCCHHHHHHHHHCCCCHHHHCCCHHCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
VENVEFKVPRVLREPA
HHCCEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA