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The map label for this gene is tesA [H]

Identifier: 110678555

GI number: 110678555

Start: 1195596

End: 1196234

Strand: Direct

Name: tesA [H]

Synonym: RD1_1228

Alternate gene names: 110678555

Gene position: 1195596-1196234 (Clockwise)

Preceding gene: 110678552

Following gene: 110678558

Centisome position: 28.93

GC content: 61.35

Gene sequence:

>639_bases
ATGCGCAAGTTAATCGGATGTTTTGTCGTTTGTTTTCCAACGTGGGGCGCTGCGGATGGCGTTGTGATCGCGGCCCTCGG
CGATAGCCTGACGCAGGGCTATGGGCTGGTGCAGGAGGAGGGCTTCGTGCCGCAACTGCAGGCCTGGCTGGACAGGGAAG
GCATCGCGGCGCGGGTGATCAACGCAGGTGTCTCGGGCGACACAACGGCTGGCGGGCTGTCGCGGGTGGCCTGGACCCTT
ACACCAAAGGTGGATGCGATGATCGTGGCCCTTGGCGGCAACGATCTTTTGCGCGGGATTGACCCGGCCGAAAGCCGCCG
CAACATCACGGGAATTCTGGAGGCGGCAGCGGCGCAGAGCGTGGAGGTGTTGCTGGTGGGCATGCAGGCACCGGGTAACT
TCGGCCCCGATTTCAAGGCGGAATTCGATGCGATTTATCCTGAGCTTGCGTCAGAATTCGGTGTTTTATACGCGGCAGAT
TTTTTTGAAGGTTTGTCCGTCGTCGGGGGCGATCCAGCGAGCGTGCGGGCATTCATGCAACCGGATGGTATTCACCCCAA
TGCACAGGGTGTCATGCGCATCGTTGAGGCGCTCGGGCCGGATGTGGCGGAACTGGTGGCACAAGCCGGATCGCGATAG

Upstream 100 bases:

>100_bases
CATCCAGTGACAATGCTGCATCTGTGAGGGTGTAGATGGGGTCAGGCATGAGATACCACAGGTTGTTGGCTTCCCTCTGG
CATATGGAGCTTGGATCAGA

Downstream 100 bases:

>100_bases
GCCCCGCTACAAAAGAAAACGGGCGCCGGAAAGCGCCCGAATGACCCGCTAAAAGCCTGTTTTACGCAATTGCAAGATCG
CTGGCGCTTTCGCGGCCATC

Product: putative acyl-CoA thioesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRKLIGCFVVCFPTWGAADGVVIAALGDSLTQGYGLVQEEGFVPQLQAWLDREGIAARVINAGVSGDTTAGGLSRVAWTL
TPKVDAMIVALGGNDLLRGIDPAESRRNITGILEAAAAQSVEVLLVGMQAPGNFGPDFKAEFDAIYPELASEFGVLYAAD
FFEGLSVVGGDPASVRAFMQPDGIHPNAQGVMRIVEALGPDVAELVAQAGSR

Sequences:

>Translated_212_residues
MRKLIGCFVVCFPTWGAADGVVIAALGDSLTQGYGLVQEEGFVPQLQAWLDREGIAARVINAGVSGDTTAGGLSRVAWTL
TPKVDAMIVALGGNDLLRGIDPAESRRNITGILEAAAAQSVEVLLVGMQAPGNFGPDFKAEFDAIYPELASEFGVLYAAD
FFEGLSVVGGDPASVRAFMQPDGIHPNAQGVMRIVEALGPDVAELVAQAGSR
>Mature_212_residues
MRKLIGCFVVCFPTWGAADGVVIAALGDSLTQGYGLVQEEGFVPQLQAWLDREGIAARVINAGVSGDTTAGGLSRVAWTL
TPKVDAMIVALGGNDLLRGIDPAESRRNITGILEAAAAQSVEVLLVGMQAPGNFGPDFKAEFDAIYPELASEFGVLYAAD
FFEGLSVVGGDPASVRAFMQPDGIHPNAQGVMRIVEALGPDVAELVAQAGSR

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=192, Percent_Identity=34.375, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 22061; Mature: 22061

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLIGCFVVCFPTWGAADGVVIAALGDSLTQGYGLVQEEGFVPQLQAWLDREGIAARVI
CCCHHEEEEEEECCCCCCCCEEEEECCCHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHE
NAGVSGDTTAGGLSRVAWTLTPKVDAMIVALGGNDLLRGIDPAESRRNITGILEAAAAQS
ECCCCCCCCCCCHHHEEEEECCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCC
VEVLLVGMQAPGNFGPDFKAEFDAIYPELASEFGVLYAADFFEGLSVVGGDPASVRAFMQ
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCHHHCCCCHHHEEEEEC
PDGIHPNAQGVMRIVEALGPDVAELVAQAGSR
CCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure
MRKLIGCFVVCFPTWGAADGVVIAALGDSLTQGYGLVQEEGFVPQLQAWLDREGIAARVI
CCCHHEEEEEEECCCCCCCCEEEEECCCHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHE
NAGVSGDTTAGGLSRVAWTLTPKVDAMIVALGGNDLLRGIDPAESRRNITGILEAAAAQS
ECCCCCCCCCCCHHHEEEEECCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCC
VEVLLVGMQAPGNFGPDFKAEFDAIYPELASEFGVLYAADFFEGLSVVGGDPASVRAFMQ
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCHHHCCCCHHHEEEEEC
PDGIHPNAQGVMRIVEALGPDVAELVAQAGSR
CCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]