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The map label for this gene is lepA [H]

Identifier: 110678419

GI number: 110678419

Start: 1035772

End: 1037571

Strand: Direct

Name: lepA [H]

Synonym: RD1_1081

Alternate gene names: 110678419

Gene position: 1035772-1037571 (Clockwise)

Preceding gene: 110678416

Following gene: 110678420

Centisome position: 25.06

GC content: 58.83

Gene sequence:

>1800_bases
ATGACACCGCTTTCACATATCCGCAATTTCTCCATCGTGGCGCATATTGACCACGGGAAATCCACGCTGGCCGACAGGCT
CATTCAGTCCACCAATACAGTGGCGGATCGGGATATGAAGGAGCAGATGCTCGACAGCATGGACATCGAGCGCGAGCGCG
GCATCACCATCAAGGCGCAGACCGTGCGGATCAATTACACCGCGCAGAATGGCGAGGAATATGTCCTCAACCTGATCGAC
ACGCCGGGGCATGTGGATTTCGCTTATGAGGTCAGCCGCTCCATGCGCGCGGTCGAGGGATCGCTGCTGGTCGTGGACAG
CACCCAGGGCGTCGAGGCGCAGACGCTGGCCAACGTCTATCACGCCATTGACGCCGATCATGAAATCGTGCCGGTGCTCA
ACAAGATCGACCTGCCTGCGTCCGATTGTGACCGTGTCGCCGAACAGATCGAAGACGTGATCGGCATCGATGCCTCCGGT
GCAATTCAGGTCTCTGCCAAGACCGGGCAGGGCATCATGGAAACGCTGGAAAGCATCGTGCAGAACCTGCCCGCGCCCAA
GGGGGACCGTGACGCGCCACTCAAGGCGATGCTGGTGGATTCGTGGTATGACAGTTACCTCGGGGTGATCGTTCTGGTGC
GCATCATGGACGGCGTGCTGAAAAAGGGCGACCGCATCCGCATGCTGTCCAACAACTCGACCCATAACGTGGACCGGATC
GGCGTCTTCCGCCCCGAGATGACGGCCATCGACGAACTGGGCCCGGGCGAGATCGGTTTCCTGACCGCGTCCATCAAGCA
GGTGCGCGACACCCGTGTGGGCGACACCATTACGCATGAGAAAAAAGGCACCGAGGTCGCGCTGCCGGGGTTCAAGCCCT
CGCAGCCTGTGGTGTTCTGCGGTCTGTTCCCGGTGGACAGCTCCGAGTTCGAGGAGTTGCGCGACAGTATCGAGAAACTG
GCGCTGAATGATGCGTCCTTTTCCTATGAGATGGAGACATCCGCCGCCCTTGGGTTCGGGTTCCGCTGTGGCTTTCTGGG
GTTGTTGCACCTCGAAGTCATCCGCGACCGGATCGAACGTGAATATGACATCGAACTGATCACCACCGCGCCCTCGGTCA
TCTACCATGTGTATATGAAAGACGGCGCGATGATCGAATTGCACAACCCCGCCGACATGCCCGACCTGACGCTGGTTGAT
CATCTGGAGGAGCCGCGGATCAAGGCGACGATCCTTGTACCCGACGACTACCTCGGCGATGTGCTGAAGCTGTGTCAGGA
CCGGCGCGGCATCCAGCAGGACCTGACCTATGCGGGCAGCCGCGCGATGGTGGTCTATGATCTGCCGCTCAACGAGGTGG
TGTTTGATTTTTATGACCGCCTGAAATCGGTGACCAAGGGCTATGCGTCCTTTGACTATGCGATGATCGGCTATCGCACG
GATAACCTTGTGAAAATGTCGATCCTTGTGAACGACGAACCGGTCGATGCGCTCTCCATCATGGTGCACCGCGAACGGGC
CGAGGGGCGGGGCCGCGCGATGGTCGAAAAGCTCAAGGACCTGATCCCGCGCCACATGTTCAAAATCCCGATTCAGGCGG
CGATTGGCGGCAAAGTCATCGCCCGCGAGACGCTGAGTGCCATGCGCAAAGACGTGACCGCCAAATGCTATGGTGGGGAT
GCGACGCGCAAACGCAAGCTGCTGGACAAGCAAAAGGCGGGGAAGAAGAAAATGCGGCAGTTTGGGAAAGTGGATATTCC
ACAGGAAGCGTTTATCAGTGCTCTCAAGATGGACGGCTAA

Upstream 100 bases:

>100_bases
CTAGCGCATCGTGACCATTCTTGCAATCACGTTCTATCAGTACAGCCCCGTTGCGCATCCACCTTGACCGCCCCCCCAAA
CCACACCATATCCCATTACC

Downstream 100 bases:

>100_bases
AGCCGTCTATCTTGCGCGCTGATAAACGACGGTGGGTGGCAGCGTTTTGCGTAGCAGGGCGCGTACCCGCACGGTGGCGT
TGGGGTGAAAGGCACGGACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTPLSHIRNFSIVAHIDHGKSTLADRLIQSTNTVADRDMKEQMLDSMDIERERGITIKAQTVRINYTAQNGEEYVLNLID
TPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYHAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASG
AIQVSAKTGQGIMETLESIVQNLPAPKGDRDAPLKAMLVDSWYDSYLGVIVLVRIMDGVLKKGDRIRMLSNNSTHNVDRI
GVFRPEMTAIDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGTEVALPGFKPSQPVVFCGLFPVDSSEFEELRDSIEKL
ALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYDIELITTAPSVIYHVYMKDGAMIELHNPADMPDLTLVD
HLEEPRIKATILVPDDYLGDVLKLCQDRRGIQQDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYAMIGYRT
DNLVKMSILVNDEPVDALSIMVHRERAEGRGRAMVEKLKDLIPRHMFKIPIQAAIGGKVIARETLSAMRKDVTAKCYGGD
ATRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG

Sequences:

>Translated_599_residues
MTPLSHIRNFSIVAHIDHGKSTLADRLIQSTNTVADRDMKEQMLDSMDIERERGITIKAQTVRINYTAQNGEEYVLNLID
TPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYHAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASG
AIQVSAKTGQGIMETLESIVQNLPAPKGDRDAPLKAMLVDSWYDSYLGVIVLVRIMDGVLKKGDRIRMLSNNSTHNVDRI
GVFRPEMTAIDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGTEVALPGFKPSQPVVFCGLFPVDSSEFEELRDSIEKL
ALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYDIELITTAPSVIYHVYMKDGAMIELHNPADMPDLTLVD
HLEEPRIKATILVPDDYLGDVLKLCQDRRGIQQDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYAMIGYRT
DNLVKMSILVNDEPVDALSIMVHRERAEGRGRAMVEKLKDLIPRHMFKIPIQAAIGGKVIARETLSAMRKDVTAKCYGGD
ATRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG
>Mature_598_residues
TPLSHIRNFSIVAHIDHGKSTLADRLIQSTNTVADRDMKEQMLDSMDIERERGITIKAQTVRINYTAQNGEEYVLNLIDT
PGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVYHAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASGA
IQVSAKTGQGIMETLESIVQNLPAPKGDRDAPLKAMLVDSWYDSYLGVIVLVRIMDGVLKKGDRIRMLSNNSTHNVDRIG
VFRPEMTAIDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGTEVALPGFKPSQPVVFCGLFPVDSSEFEELRDSIEKLA
LNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIEREYDIELITTAPSVIYHVYMKDGAMIELHNPADMPDLTLVDH
LEEPRIKATILVPDDYLGDVLKLCQDRRGIQQDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYAMIGYRTD
NLVKMSILVNDEPVDALSIMVHRERAEGRGRAMVEKLKDLIPRHMFKIPIQAAIGGKVIARETLSAMRKDVTAKCYGGDA
TRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=48.2587064676617, Blast_Score=606, Evalue=1e-173,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=37.9310344827586, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=34.3558282208589, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=43.2432432432432, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=43.2432432432432, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=43.2432432432432, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI25306283, Length=155, Percent_Identity=40.6451612903226, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=155, Percent_Identity=40.6451612903226, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306287, Length=155, Percent_Identity=40.6451612903226, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217272892, Length=146, Percent_Identity=34.9315068493151, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI217272894, Length=146, Percent_Identity=34.9315068493151, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI53729339, Length=254, Percent_Identity=26.3779527559055, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI53729337, Length=254, Percent_Identity=26.3779527559055, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=54.8986486486487, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=507, Percent_Identity=28.0078895463511, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=171, Percent_Identity=29.8245614035088, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789559, Length=297, Percent_Identity=28.2828282828283, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.1639344262295, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=457, Percent_Identity=24.72647702407, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506493, Length=219, Percent_Identity=31.5068493150685, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=37.5, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=37.5, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=141, Percent_Identity=36.8794326241135, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566303, Length=310, Percent_Identity=22.9032258064516, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=223, Percent_Identity=26.457399103139, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=44.6102819237148, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=166, Percent_Identity=37.9518072289157, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=37.3333333333333, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=37.3333333333333, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=139, Percent_Identity=37.410071942446, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=172, Percent_Identity=34.8837209302326, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=330, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=44.4444444444444, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=35.5828220858896, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.1654676258993, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.1621621621622, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=37.037037037037, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221458488, Length=154, Percent_Identity=38.3116883116883, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66742; Mature: 66610

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLSHIRNFSIVAHIDHGKSTLADRLIQSTNTVADRDMKEQMLDSMDIERERGITIKAQ
CCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCEEEEE
TVRINYTAQNGEEYVLNLIDTPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
HAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASGAIQVSAKTGQGIMETLESIV
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
QNLPAPKGDRDAPLKAMLVDSWYDSYLGVIVLVRIMDGVLKKGDRIRMLSNNSTHNVDRI
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
GVFRPEMTAIDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGTEVALPGFKPSQPVVFC
EECCCCHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEEE
GLFPVDSSEFEELRDSIEKLALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIER
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCC
EYDIELITTAPSVIYHVYMKDGAMIELHNPADMPDLTLVDHLEEPRIKATILVPDDYLGD
CCCEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCHHHHH
VLKLCQDRRGIQQDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYAMIGYRT
HHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCEEEEEECC
DNLVKMSILVNDEPVDALSIMVHRERAEGRGRAMVEKLKDLIPRHMFKIPIQAAIGGKVI
CCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
ARETLSAMRKDVTAKCYGGDATRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TPLSHIRNFSIVAHIDHGKSTLADRLIQSTNTVADRDMKEQMLDSMDIERERGITIKAQ
CCHHHHCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCEEEEE
TVRINYTAQNGEEYVLNLIDTPGHVDFAYEVSRSMRAVEGSLLVVDSTQGVEAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
HAIDADHEIVPVLNKIDLPASDCDRVAEQIEDVIGIDASGAIQVSAKTGQGIMETLESIV
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
QNLPAPKGDRDAPLKAMLVDSWYDSYLGVIVLVRIMDGVLKKGDRIRMLSNNSTHNVDRI
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
GVFRPEMTAIDELGPGEIGFLTASIKQVRDTRVGDTITHEKKGTEVALPGFKPSQPVVFC
EECCCCHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCEEEE
GLFPVDSSEFEELRDSIEKLALNDASFSYEMETSAALGFGFRCGFLGLLHLEVIRDRIER
EEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCC
EYDIELITTAPSVIYHVYMKDGAMIELHNPADMPDLTLVDHLEEPRIKATILVPDDYLGD
CCCEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCHHHHCCCCCCEEEEEECCCHHHHH
VLKLCQDRRGIQQDLTYAGSRAMVVYDLPLNEVVFDFYDRLKSVTKGYASFDYAMIGYRT
HHHHHHHHCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCEEEEEECC
DNLVKMSILVNDEPVDALSIMVHRERAEGRGRAMVEKLKDLIPRHMFKIPIQAAIGGKVI
CCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHH
ARETLSAMRKDVTAKCYGGDATRKRKLLDKQKAGKKKMRQFGKVDIPQEAFISALKMDG
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA