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The map label for this gene is slt [H]

Identifier: 110678317

GI number: 110678317

Start: 933430

End: 935343

Strand: Reverse

Name: slt [H]

Synonym: RD1_0971

Alternate gene names: 110678317

Gene position: 935343-933430 (Counterclockwise)

Preceding gene: 110678319

Following gene: 110678315

Centisome position: 22.63

GC content: 60.61

Gene sequence:

>1914_bases
GTGTCCGCCGACAGACCGCGCCCACTGGGGTGGGCATTGGATGCGGCGCGCAACGGGAATTGGCAGGCTGCAGCGCAGAT
CGCGGCACGCGATGGGCAAGTGGCCGCTGATGTCATTGAATGGATGCGCCTGCGCGATGGGCTTGGCACCTATGATGAGG
TGGCGGCATTCCTTGAACGTCGTCCTGACTGGCCGGGCGAGCGGTATTTGAGGCGGCAATCCGAAACCACGCTGATGGCG
CAAAGCGATGACCGGGTGATCGCATTTTTCGAGGAAACACCTCCGCAATCGCCTCAAAGCGCGTTGCGTTTCGCAAAGGC
CCTCAAAGCGCGCCAACGCACCGAAGAGGCGGAGGCCATCATCACAGGCGCGTGGCGCAACATGCCGATGGGTGCTTCGG
ATCAGGCGGAATACATTGCCGCTCACGGGCGCATTTTGACGGCGCATCACGACGCACGCCTTGATTCGATGCTCTGGCAA
GGCGCTGTCGGAAATGCGCGCAGGATGTTTGATCTGGCCTCGAAAGGGGCGATTGCAGCAGCCAAGGCGCGGCTGGCCCT
GCGCGAAGAGACCCCCGGCGTCGATACCCTGATCGCGGCTGTGCCGGCCAGCAACGCAAAGGATGGTGGCCTGCAGTATG
AGCGGTTCATGTGGCGCATCCGCAAAGGTCGCCGCGATGATGCCAAGGAGTTGATGCTGGCCACCTCGGTCAGTGCCGAC
CGTTTGGGCCGCCCCGAAGCATGGTCGAATCGCAGACGTGCACTGGCGCGGTTTGAAATGCGAAACGGCAGCGCCGAACG
GGCATATGAACTGGCGTCGCGGCATTTTCTGACAGGCGGGTCTGCCTATTCCGATCTGGAGTGGTTGGCGGGCTACATCG
CCTTGCAAAAGCTGAAGGAACCGCAGACCGCCCTGAAACACTTTGAAAACCATGATGCGGCTGTGCGCTCACCAATATCG
AAGGGACGCGCGGGGTATTGGCGTGGTCGCGCCTATGAAGCCATGGGCAATGTCGCAGCAGCTGATCAGGCCTACGCGTC
TGCTGCGCAATACCAAACATCTTTCTATGGTTTGCTCGCGGCAGAGCGGGCAGGGCTTCCCTTCGACATTGAACTCGCCG
TTTCCGAACCGGAAACGGATTGGCGCAGTTCGGATCTTGCAAGAACACCCTTGTTCGAAGCCGGTTTGCTCTTGCAAGCC
TCAGGGGAGCTCAATCTCGCTGAACGGTTCTGGACACATCTTGCGGAGAGCCTCGATGCGCAAAACGGCGCGTTACTGGC
CAAGGCCGCCGTTGATGCCGGGCAGCCGCACCTGGCCGTCATGATTGGCAAACGCCTTGCCCAGCGGGCCATCGTCATAC
CCTACGGTTACTACGCCTTGCACCCGCTGGCAGAGCGCGCTCTGCCGATGGCACCCGAAATGTTGCTGGCAATTGCCCGC
CGGGAAAGCGAGTTCGACCCCCGCGTCCAAAGCGGCGTAGGCGCAAGGGGACTGATGCAAATCATGCCCCGCACCGGCAC
TGAAGTTGCCCGCAAACTCGGACGCGCCGCAGAACATAGCACGGAACGTCTGATCTCGGACCCGGTCTATAATGCCGAGT
TGGGTGCGGCCTATCTGGCAACGCTGGCGCGACGGTTCTCGGGCAATGTGATCATGATGTCGGCGGGGTACAACGCTGGT
CCCAGCCGACCGGACCAATGGATGGACGTTTACGGTGATCCGCGTCGCGGTGATGTGGATATCGTGGATTGGATCGAACA
CATTCCGTTTCGCGAAACGCGCAACTATGTCATGCGTGTCTCGGAAAGCTTACCGATCTACCGCGCCCAGCTAGGAAAAA
ATCCGCTTCCCATTCCCTTCAGCAAGGAACTGACGGGGTCGACCCTATTAGACTACGCACCGAAAGGAGAGTGA

Upstream 100 bases:

>100_bases
GCAATAAGTATGAGAGCCTCTAGCCTTCAAACCCTAGGAAATGCAAGTGATCAGGCGTTTATTCATCAGTCTTTTACTGG
TTTTGCTCACCGCTTTACCT

Downstream 100 bases:

>100_bases
CTCACCAACCGGTGGCCCGTTGAGCGGGATCGCGCAAAACCCCGCAACTCATGTGCCTTTTCCGGCCATATCCCGCGATG
CGCTGCACATCAAAACCCGC

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSADRPRPLGWALDAARNGNWQAAAQIAARDGQVAADVIEWMRLRDGLGTYDEVAAFLERRPDWPGERYLRRQSETTLMA
QSDDRVIAFFEETPPQSPQSALRFAKALKARQRTEEAEAIITGAWRNMPMGASDQAEYIAAHGRILTAHHDARLDSMLWQ
GAVGNARRMFDLASKGAIAAAKARLALREETPGVDTLIAAVPASNAKDGGLQYERFMWRIRKGRRDDAKELMLATSVSAD
RLGRPEAWSNRRRALARFEMRNGSAERAYELASRHFLTGGSAYSDLEWLAGYIALQKLKEPQTALKHFENHDAAVRSPIS
KGRAGYWRGRAYEAMGNVAAADQAYASAAQYQTSFYGLLAAERAGLPFDIELAVSEPETDWRSSDLARTPLFEAGLLLQA
SGELNLAERFWTHLAESLDAQNGALLAKAAVDAGQPHLAVMIGKRLAQRAIVIPYGYYALHPLAERALPMAPEMLLAIAR
RESEFDPRVQSGVGARGLMQIMPRTGTEVARKLGRAAEHSTERLISDPVYNAELGAAYLATLARRFSGNVIMMSAGYNAG
PSRPDQWMDVYGDPRRGDVDIVDWIEHIPFRETRNYVMRVSESLPIYRAQLGKNPLPIPFSKELTGSTLLDYAPKGE

Sequences:

>Translated_637_residues
MSADRPRPLGWALDAARNGNWQAAAQIAARDGQVAADVIEWMRLRDGLGTYDEVAAFLERRPDWPGERYLRRQSETTLMA
QSDDRVIAFFEETPPQSPQSALRFAKALKARQRTEEAEAIITGAWRNMPMGASDQAEYIAAHGRILTAHHDARLDSMLWQ
GAVGNARRMFDLASKGAIAAAKARLALREETPGVDTLIAAVPASNAKDGGLQYERFMWRIRKGRRDDAKELMLATSVSAD
RLGRPEAWSNRRRALARFEMRNGSAERAYELASRHFLTGGSAYSDLEWLAGYIALQKLKEPQTALKHFENHDAAVRSPIS
KGRAGYWRGRAYEAMGNVAAADQAYASAAQYQTSFYGLLAAERAGLPFDIELAVSEPETDWRSSDLARTPLFEAGLLLQA
SGELNLAERFWTHLAESLDAQNGALLAKAAVDAGQPHLAVMIGKRLAQRAIVIPYGYYALHPLAERALPMAPEMLLAIAR
RESEFDPRVQSGVGARGLMQIMPRTGTEVARKLGRAAEHSTERLISDPVYNAELGAAYLATLARRFSGNVIMMSAGYNAG
PSRPDQWMDVYGDPRRGDVDIVDWIEHIPFRETRNYVMRVSESLPIYRAQLGKNPLPIPFSKELTGSTLLDYAPKGE
>Mature_636_residues
SADRPRPLGWALDAARNGNWQAAAQIAARDGQVAADVIEWMRLRDGLGTYDEVAAFLERRPDWPGERYLRRQSETTLMAQ
SDDRVIAFFEETPPQSPQSALRFAKALKARQRTEEAEAIITGAWRNMPMGASDQAEYIAAHGRILTAHHDARLDSMLWQG
AVGNARRMFDLASKGAIAAAKARLALREETPGVDTLIAAVPASNAKDGGLQYERFMWRIRKGRRDDAKELMLATSVSADR
LGRPEAWSNRRRALARFEMRNGSAERAYELASRHFLTGGSAYSDLEWLAGYIALQKLKEPQTALKHFENHDAAVRSPISK
GRAGYWRGRAYEAMGNVAAADQAYASAAQYQTSFYGLLAAERAGLPFDIELAVSEPETDWRSSDLARTPLFEAGLLLQAS
GELNLAERFWTHLAESLDAQNGALLAKAAVDAGQPHLAVMIGKRLAQRAIVIPYGYYALHPLAERALPMAPEMLLAIARR
ESEFDPRVQSGVGARGLMQIMPRTGTEVARKLGRAAEHSTERLISDPVYNAELGAAYLATLARRFSGNVIMMSAGYNAGP
SRPDQWMDVYGDPRRGDVDIVDWIEHIPFRETRNYVMRVSESLPIYRAQLGKNPLPIPFSKELTGSTLLDYAPKGE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=293, Percent_Identity=31.3993174061433, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 70527; Mature: 70395

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADRPRPLGWALDAARNGNWQAAAQIAARDGQVAADVIEWMRLRDGLGTYDEVAAFLER
CCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
RPDWPGERYLRRQSETTLMAQSDDRVIAFFEETPPQSPQSALRFAKALKARQRTEEAEAI
CCCCCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHE
ITGAWRNMPMGASDQAEYIAAHGRILTAHHDARLDSMLWQGAVGNARRMFDLASKGAIAA
EEECCCCCCCCCCCCHHHHHHCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
AKARLALREETPGVDTLIAAVPASNAKDGGLQYERFMWRIRKGRRDDAKELMLATSVSAD
HHHHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
RLGRPEAWSNRRRALARFEMRNGSAERAYELASRHFLTGGSAYSDLEWLAGYIALQKLKE
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCC
PQTALKHFENHDAAVRSPISKGRAGYWRGRAYEAMGNVAAADQAYASAAQYQTSFYGLLA
HHHHHHHHHCCCHHHHCCHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AERAGLPFDIELAVSEPETDWRSSDLARTPLFEAGLLLQASGELNLAERFWTHLAESLDA
HHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHCC
QNGALLAKAAVDAGQPHLAVMIGKRLAQRAIVIPYGYYALHPLAERALPMAPEMLLAIAR
CCCCEEEEHHHCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHH
RESEFDPRVQSGVGARGLMQIMPRTGTEVARKLGRAAEHSTERLISDPVYNAELGAAYLA
HCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
TLARRFSGNVIMMSAGYNAGPSRPDQWMDVYGDPRRGDVDIVDWIEHIPFRETRNYVMRV
HHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
SESLPIYRAQLGKNPLPIPFSKELTGSTLLDYAPKGE
HCCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SADRPRPLGWALDAARNGNWQAAAQIAARDGQVAADVIEWMRLRDGLGTYDEVAAFLER
CCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
RPDWPGERYLRRQSETTLMAQSDDRVIAFFEETPPQSPQSALRFAKALKARQRTEEAEAI
CCCCCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHE
ITGAWRNMPMGASDQAEYIAAHGRILTAHHDARLDSMLWQGAVGNARRMFDLASKGAIAA
EEECCCCCCCCCCCCHHHHHHCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHH
AKARLALREETPGVDTLIAAVPASNAKDGGLQYERFMWRIRKGRRDDAKELMLATSVSAD
HHHHHHHHCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
RLGRPEAWSNRRRALARFEMRNGSAERAYELASRHFLTGGSAYSDLEWLAGYIALQKLKE
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCC
PQTALKHFENHDAAVRSPISKGRAGYWRGRAYEAMGNVAAADQAYASAAQYQTSFYGLLA
HHHHHHHHHCCCHHHHCCHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AERAGLPFDIELAVSEPETDWRSSDLARTPLFEAGLLLQASGELNLAERFWTHLAESLDA
HHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHCC
QNGALLAKAAVDAGQPHLAVMIGKRLAQRAIVIPYGYYALHPLAERALPMAPEMLLAIAR
CCCCEEEEHHHCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHH
RESEFDPRVQSGVGARGLMQIMPRTGTEVARKLGRAAEHSTERLISDPVYNAELGAAYLA
HCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
TLARRFSGNVIMMSAGYNAGPSRPDQWMDVYGDPRRGDVDIVDWIEHIPFRETRNYVMRV
HHHHHHCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
SESLPIYRAQLGKNPLPIPFSKELTGSTLLDYAPKGE
HCCCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]