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The map label for this gene is mst [H]

Identifier: 110678281

GI number: 110678281

Start: 902853

End: 903707

Strand: Direct

Name: mst [H]

Synonym: RD1_0934

Alternate gene names: 110678281

Gene position: 902853-903707 (Clockwise)

Preceding gene: 110678280

Following gene: 110678282

Centisome position: 21.84

GC content: 60.35

Gene sequence:

>855_bases
ATGATTGATGATCCGAAAACACTCGTTTCGACCGAATGGCTGGCAGAGCATATGAAAGACCCTGACCTGCGTATTCTGGA
TGCGTCCTGGTATCTGCCAGAGGCCGGGCGGCACGCCAAACAGGAATATGACGAAGGCCACATCCCCGGCGCGCGCTTCT
TTGATATCGACGAAATATCGGACAGCCGTTCTGATCTGCCACATATGGCGCCGCGCAGCGAGAAATTCACCTCCCGGATG
CGTGCGCTTGGTGTGGGTGATGGCCATCAGGTGGTTGTCTATGACGGGGCGGGCTTGCTCTCCGCGCCGCGGGTGTGGTG
GCTTTTCCGCCTGATGGGGCAACAGAGTGTTGCTGTTCTGGACGGCGGTTTGCCGAAATGGAAAGCGGAAGGGCGCGAGA
TCGAGGATATGCCGCCCATCGTGCGGGACCGCCACATCACTGTCCGTTTCCAGAACCATCTGGTGCGCGATGTCACGCAG
GTGTCGGCTGCGTCCAAGATCAAGGATCACGTCATTGTCGATGCGCGCGCAGGCAACCGGTTTCGTGGCGAAGCACCTGA
GCCGCGTGAAGGCTTGCGGGCGGGCCATATCCCCGGCTCTCGCTCTGTTCCATATTCCACGCTGTTGAATGAGGACCAGA
CGATGAAGTCGCCTGACGACTGCAGACAGATATTCGAGGCGGCAGGGGTGGATCTGGATAAACCGATCATCACCTCCTGC
GGGTCCGGGATCACGGCGGCGGTCCTCGCGCTGGCGCTGGAACGCATGGGACACCGCCATTGGTCCTTGTACGATGGGTC
CTGGGCCGAATGGGGGATGTTTCCCACCGTGCCCGTCGCGACAGGAGACGCATGA

Upstream 100 bases:

>100_bases
AAAGATCACGGCTGAACCATGCGCTGACTTGGGGTACGGGCTTGCCAGTCCAGCCCTGTGACGTTACCCAGAACCGGTCC
TAGAGCGCAGGGGTAAGCTG

Downstream 100 bases:

>100_bases
TGTTCGAGACCCTCAAGGCGCGCCCTGCCGACGGAATCCTCGCATTGATGCAGATGTTCAAAGACGATCCGCGCGACAAC
AAGATCGACCTTGGCGTCGG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MIDDPKTLVSTEWLAEHMKDPDLRILDASWYLPEAGRHAKQEYDEGHIPGARFFDIDEISDSRSDLPHMAPRSEKFTSRM
RALGVGDGHQVVVYDGAGLLSAPRVWWLFRLMGQQSVAVLDGGLPKWKAEGREIEDMPPIVRDRHITVRFQNHLVRDVTQ
VSAASKIKDHVIVDARAGNRFRGEAPEPREGLRAGHIPGSRSVPYSTLLNEDQTMKSPDDCRQIFEAAGVDLDKPIITSC
GSGITAAVLALALERMGHRHWSLYDGSWAEWGMFPTVPVATGDA

Sequences:

>Translated_284_residues
MIDDPKTLVSTEWLAEHMKDPDLRILDASWYLPEAGRHAKQEYDEGHIPGARFFDIDEISDSRSDLPHMAPRSEKFTSRM
RALGVGDGHQVVVYDGAGLLSAPRVWWLFRLMGQQSVAVLDGGLPKWKAEGREIEDMPPIVRDRHITVRFQNHLVRDVTQ
VSAASKIKDHVIVDARAGNRFRGEAPEPREGLRAGHIPGSRSVPYSTLLNEDQTMKSPDDCRQIFEAAGVDLDKPIITSC
GSGITAAVLALALERMGHRHWSLYDGSWAEWGMFPTVPVATGDA
>Mature_284_residues
MIDDPKTLVSTEWLAEHMKDPDLRILDASWYLPEAGRHAKQEYDEGHIPGARFFDIDEISDSRSDLPHMAPRSEKFTSRM
RALGVGDGHQVVVYDGAGLLSAPRVWWLFRLMGQQSVAVLDGGLPKWKAEGREIEDMPPIVRDRHITVRFQNHLVRDVTQ
VSAASKIKDHVIVDARAGNRFRGEAPEPREGLRAGHIPGSRSVPYSTLLNEDQTMKSPDDCRQIFEAAGVDLDKPIITSC
GSGITAAVLALALERMGHRHWSLYDGSWAEWGMFPTVPVATGDA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=41.2408759124088, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=41.2408759124088, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=41.2408759124088, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=40.6593406593407, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI87082121, Length=273, Percent_Identity=45.0549450549451, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI87081967, Length=289, Percent_Identity=28.719723183391, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561888, Length=290, Percent_Identity=27.2413793103448, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71997283, Length=305, Percent_Identity=26.5573770491803, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115534702, Length=232, Percent_Identity=27.1551724137931, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543836, Length=292, Percent_Identity=27.0547945205479, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=292, Percent_Identity=27.0547945205479, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=248, Percent_Identity=25, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=265, Percent_Identity=33.5849056603774, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31612; Mature: 31612

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDPKTLVSTEWLAEHMKDPDLRILDASWYLPEAGRHAKQEYDEGHIPGARFFDIDEIS
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEHHHHC
DSRSDLPHMAPRSEKFTSRMRALGVGDGHQVVVYDGAGLLSAPRVWWLFRLMGQQSVAVL
CCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEE
DGGLPKWKAEGREIEDMPPIVRDRHITVRFQNHLVRDVTQVSAASKIKDHVIVDARAGNR
CCCCCCCCCCCCCCCCCCCCHHCCEEEEEEHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
FRGEAPEPREGLRAGHIPGSRSVPYSTLLNEDQTMKSPDDCRQIFEAAGVDLDKPIITSC
CCCCCCCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHC
GSGITAAVLALALERMGHRHWSLYDGSWAEWGMFPTVPVATGDA
CCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDDPKTLVSTEWLAEHMKDPDLRILDASWYLPEAGRHAKQEYDEGHIPGARFFDIDEIS
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEHHHHC
DSRSDLPHMAPRSEKFTSRMRALGVGDGHQVVVYDGAGLLSAPRVWWLFRLMGQQSVAVL
CCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEE
DGGLPKWKAEGREIEDMPPIVRDRHITVRFQNHLVRDVTQVSAASKIKDHVIVDARAGNR
CCCCCCCCCCCCCCCCCCCCHHCCEEEEEEHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
FRGEAPEPREGLRAGHIPGSRSVPYSTLLNEDQTMKSPDDCRQIFEAAGVDLDKPIITSC
CCCCCCCHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHC
GSGITAAVLALALERMGHRHWSLYDGSWAEWGMFPTVPVATGDA
CCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]