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The map label for this gene is smpB [H]

Identifier: 110678280

GI number: 110678280

Start: 902105

End: 902767

Strand: Direct

Name: smpB [H]

Synonym: RD1_0933

Alternate gene names: 110678280

Gene position: 902105-902767 (Clockwise)

Preceding gene: 110678278

Following gene: 110678281

Centisome position: 21.83

GC content: 56.86

Gene sequence:

>663_bases
ATGCCTACATGTATGCGCCGATCCGCCAATGCGACCGTCCGGGCGACACAGGCTGGCGGGTTCAAACCCGGGATGCACCA
ACGCGGGCATGCTATCAAAACACCTGATCGAGGTGCCTCAGCCGACAGGTGTCATGTGCTTGCACCATCGCAGCAAACTG
CCTACATCACGGGCATGGCACAGGTGAAATCATCCTCCAAATCCGATCCGAACTACAAGGTTGTCGCAGAGAATCGCCGC
GCGCGGTTTGACTACGCCATTGAAAGCGATGTGGAATGCGGCATCATTCTGGAAGGCTCCGAAGTGAAATCCCTGCGCGA
GGGTGGTGCCAATATCGCGGAAAGCTATGCGGCAGTGGAGGACGGTGAACTGTGGCTCGTCAACAGCTATATCGCCCCCT
ACAAACAGGCCAAAACATTTCAGCATGAGGAACGCCGCCGCCGCAAGCTGCTGATCAGCCGCAAGCAATTGTCGGATTTC
TGGAATGCGACACAGCGCAAAGGCATGACGCTGGTGCCGCTGGTGCTTTACTTCAACCACCGGGGCATGGCCAAGATCAA
GATCGGCGTGGCCAAGGGTAAAAAGCTGCATGACAAACGCGAAACGGCTGCCAAACGCGACTGGTCGCGGCAAAAGTCCC
GGTTGATGAAAGATCACGGCTGA

Upstream 100 bases:

>100_bases
CGTAAGAATAGCTCATGGGTCCCTTTCGAACTGCAAACTGTTTTGATCATGTGCACTATCGCAGGCAATTGCGCGAGGAA
CAGAGGGAAACTGCGCAAAC

Downstream 100 bases:

>100_bases
ACCATGCGCTGACTTGGGGTACGGGCTTGCCAGTCCAGCCCTGTGACGTTACCCAGAACCGGTCCTAGAGCGCAGGGGTA
AGCTGATGATTGATGATCCG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MPTCMRRSANATVRATQAGGFKPGMHQRGHAIKTPDRGASADRCHVLAPSQQTAYITGMAQVKSSSKSDPNYKVVAENRR
ARFDYAIESDVECGIILEGSEVKSLREGGANIAESYAAVEDGELWLVNSYIAPYKQAKTFQHEERRRRKLLISRKQLSDF
WNATQRKGMTLVPLVLYFNHRGMAKIKIGVAKGKKLHDKRETAAKRDWSRQKSRLMKDHG

Sequences:

>Translated_220_residues
MPTCMRRSANATVRATQAGGFKPGMHQRGHAIKTPDRGASADRCHVLAPSQQTAYITGMAQVKSSSKSDPNYKVVAENRR
ARFDYAIESDVECGIILEGSEVKSLREGGANIAESYAAVEDGELWLVNSYIAPYKQAKTFQHEERRRRKLLISRKQLSDF
WNATQRKGMTLVPLVLYFNHRGMAKIKIGVAKGKKLHDKRETAAKRDWSRQKSRLMKDHG
>Mature_219_residues
PTCMRRSANATVRATQAGGFKPGMHQRGHAIKTPDRGASADRCHVLAPSQQTAYITGMAQVKSSSKSDPNYKVVAENRRA
RFDYAIESDVECGIILEGSEVKSLREGGANIAESYAAVEDGELWLVNSYIAPYKQAKTFQHEERRRRKLLISRKQLSDFW
NATQRKGMTLVPLVLYFNHRGMAKIKIGVAKGKKLHDKRETAAKRDWSRQKSRLMKDHG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=150, Percent_Identity=44, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 24762; Mature: 24630

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTCMRRSANATVRATQAGGFKPGMHQRGHAIKTPDRGASADRCHVLAPSQQTAYITGMA
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCCCEEEEHHH
QVKSSSKSDPNYKVVAENRRARFDYAIESDVECGIILEGSEVKSLREGGANIAESYAAVE
HHHCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECHHHHHHHHCCCHHHHHHHHCC
DGELWLVNSYIAPYKQAKTFQHEERRRRKLLISRKQLSDFWNATQRKGMTLVPLVLYFNH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
RGMAKIKIGVAKGKKLHDKRETAAKRDWSRQKSRLMKDHG
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTCMRRSANATVRATQAGGFKPGMHQRGHAIKTPDRGASADRCHVLAPSQQTAYITGMA
CCCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCCCEEEEHHH
QVKSSSKSDPNYKVVAENRRARFDYAIESDVECGIILEGSEVKSLREGGANIAESYAAVE
HHHCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECHHHHHHHHCCCHHHHHHHHCC
DGELWLVNSYIAPYKQAKTFQHEERRRRKLLISRKQLSDFWNATQRKGMTLVPLVLYFNH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
RGMAKIKIGVAKGKKLHDKRETAAKRDWSRQKSRLMKDHG
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA