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The map label for this gene is flhA [H]

Identifier: 110678234

GI number: 110678234

Start: 863195

End: 864307

Strand: Direct

Name: flhA [H]

Synonym: RD1_0887

Alternate gene names: 110678234

Gene position: 863195-864307 (Clockwise)

Preceding gene: 110678232

Following gene: 110678235

Centisome position: 20.88

GC content: 62.08

Gene sequence:

>1113_bases
ATGAGAACGCGAGCGGCGGTTGCGGTTGCGGCTGGGCAGCCTCTTGAGGTGATGGAGGTCAATCTTGATGGCCCGCGCGC
CGGAGAGGTTCTGGTCGAGATCAAGGCGACGGGTCTGTGTCATACGGATGAGTTCACGCGCTCGGGCGATGACCCCGAAG
GTATCTTCCCGGCAATCCTCGGCCATGAGGGCGCGGGCGTTGTCATCGAGGTGGGCGAGGGGGTGACCAGCCTTGAAGTG
GGCGATCACGTGATCCCGCTTTACACGCCGGAGTGTCGCGAATGCGACTACTGCCTGAACCCCAAGACCAACCTCTGCCA
GAAGGTGCGTGCCACGCAGGGCGCGGGGCTGTTGCCTGATGGCTCAACCCGGTTTTCGATGCTGGATGGCACGCCCATTC
ACCATTACATGGGCTGTTCGACCTTTGCCAACCACACGGTTGTGCCCGAAATCGCGCTGGCCAAGGTGCGCAAGGACGCG
CCCTTCGACAAGATCTGCTACATCGGGTGCGGCGTGACCACCGGCATCGGTGCCGTCATTAACACGGCAAAGGTTGAGAT
TGGGTCAACGGCCGCCGTGTTTGGCCTCGGTGGCATCGGTCTCAATGTGATCCAGGGGCTGCGCATGGCGGGCGCGGATC
AGATCATCGGCGTCGATCTCAACGACGACAAGGAAAAAATGGCGCGCCATTTCGGCATGACGGATTTCGTCAACCCCAGC
AAGGTCGAGGGCGACATGGTCGCGCATCTGGTCGAACTGTCGGGTGGGGGCTGTGACTACACCTTTGACGCCACGGGCAA
TGTGAACGTGATGCGCACCGCTCTGGAGGCTGCGCACAAGGGCTGGGGCGAGAGCATCATCATCGGCGTGGCCCCCGCGG
GTGCGGAGATTGCGACGCGGCCCTTCCAACTGGTGACGGGCCGGGTCTGGCGCGGCACGGCCTTCGGCGGGGCCAAGGGG
CGCACGGATGTGCCGAAAATCGTGGACTGGTACATGGACGGCAAGATCGAGATCGACCCCATGATCACCCACAAACTCAG
CCTGGACGAAATCAACCACGGCTTCGACCTCATGCACCAGGGAAAATCAATCAGAGCCGTCGTGGAGTTTTGA

Upstream 100 bases:

>100_bases
ACCCACCATTGTCTTAGTTGTTCTGAAGCTTGCTCGGCCCGCATACTCTGGTGGAGTACGGTAGCATGCCAATGTGCTGC
ACCTTTGGGAGACGATTATG

Downstream 100 bases:

>100_bases
GAGCAGAGATGGAGCTGATATCAGAAAACCGGAGTTTTGGCGGCAGCCAGCAGGTCTTCAAGCACCCGTCGAACACGTGC
GGCTGCGAGATGACATTCGC

Product: glutathione-dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRTRAAVAVAAGQPLEVMEVNLDGPRAGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAILGHEGAGVVIEVGEGVTSLEV
GDHVIPLYTPECRECDYCLNPKTNLCQKVRATQGAGLLPDGSTRFSMLDGTPIHHYMGCSTFANHTVVPEIALAKVRKDA
PFDKICYIGCGVTTGIGAVINTAKVEIGSTAAVFGLGGIGLNVIQGLRMAGADQIIGVDLNDDKEKMARHFGMTDFVNPS
KVEGDMVAHLVELSGGGCDYTFDATGNVNVMRTALEAAHKGWGESIIIGVAPAGAEIATRPFQLVTGRVWRGTAFGGAKG
RTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMHQGKSIRAVVEF

Sequences:

>Translated_370_residues
MRTRAAVAVAAGQPLEVMEVNLDGPRAGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAILGHEGAGVVIEVGEGVTSLEV
GDHVIPLYTPECRECDYCLNPKTNLCQKVRATQGAGLLPDGSTRFSMLDGTPIHHYMGCSTFANHTVVPEIALAKVRKDA
PFDKICYIGCGVTTGIGAVINTAKVEIGSTAAVFGLGGIGLNVIQGLRMAGADQIIGVDLNDDKEKMARHFGMTDFVNPS
KVEGDMVAHLVELSGGGCDYTFDATGNVNVMRTALEAAHKGWGESIIIGVAPAGAEIATRPFQLVTGRVWRGTAFGGAKG
RTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMHQGKSIRAVVEF
>Mature_370_residues
MRTRAAVAVAAGQPLEVMEVNLDGPRAGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAILGHEGAGVVIEVGEGVTSLEV
GDHVIPLYTPECRECDYCLNPKTNLCQKVRATQGAGLLPDGSTRFSMLDGTPIHHYMGCSTFANHTVVPEIALAKVRKDA
PFDKICYIGCGVTTGIGAVINTAKVEIGSTAAVFGLGGIGLNVIQGLRMAGADQIIGVDLNDDKEKMARHFGMTDFVNPS
KVEGDMVAHLVELSGGGCDYTFDATGNVNVMRTALEAAHKGWGESIIIGVAPAGAEIATRPFQLVTGRVWRGTAFGGAKG
RTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMHQGKSIRAVVEF

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=370, Percent_Identity=58.6486486486486, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI34577061, Length=370, Percent_Identity=44.0540540540541, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=44.0540540540541, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=44.0540540540541, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=44, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI4501933, Length=370, Percent_Identity=43.7837837837838, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI4501929, Length=370, Percent_Identity=43.5135135135135, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=41.8478260869565, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI4501939, Length=362, Percent_Identity=41.9889502762431, Blast_Score=312, Evalue=3e-85,
Organism=Escherichia coli, GI1786552, Length=370, Percent_Identity=60.2702702702703, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1790045, Length=353, Percent_Identity=25.4957507082153, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082125, Length=383, Percent_Identity=24.5430809399478, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=24.0687679083095, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081918, Length=370, Percent_Identity=27.8378378378378, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=358, Percent_Identity=25.4189944134078, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786825, Length=265, Percent_Identity=28.6792452830189, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI226510992, Length=369, Percent_Identity=23.8482384823848, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.7956403269755, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788407, Length=226, Percent_Identity=28.7610619469027, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=374, Percent_Identity=55.6149732620321, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71997431, Length=376, Percent_Identity=55.3191489361702, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17565904, Length=344, Percent_Identity=55.2325581395349, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562584, Length=368, Percent_Identity=27.1739130434783, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=27.1505376344086, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=373, Percent_Identity=25.7372654155496, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=372, Percent_Identity=51.8817204301075, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319955, Length=361, Percent_Identity=27.7008310249307, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=361, Percent_Identity=27.7008310249307, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=362, Percent_Identity=25.6906077348066, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=26.3736263736264, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=377, Percent_Identity=28.3819628647215, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323099, Length=380, Percent_Identity=27.6315789473684, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=25.2054794520548, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319258, Length=278, Percent_Identity=27.3381294964029, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=276, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17737895, Length=369, Percent_Identity=55.8265582655827, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17137530, Length=342, Percent_Identity=25.4385964912281, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=25.2199413489736, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39316; Mature: 39316

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRAAVAVAAGQPLEVMEVNLDGPRAGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCCEECCHHHCCCCCCCCCCHHHH
GHEGAGVVIEVGEGVTSLEVGDHVIPLYTPECRECDYCLNPKTNLCQKVRATQGAGLLPD
CCCCCEEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GSTRFSMLDGTPIHHYMGCSTFANHTVVPEIALAKVRKDAPFDKICYIGCGVTTGIGAVI
CCCEEEEECCCCHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHE
NTAKVEIGSTAAVFGLGGIGLNVIQGLRMAGADQIIGVDLNDDKEKMARHFGMTDFVNPS
EEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCC
KVEGDMVAHLVELSGGGCDYTFDATGNVNVMRTALEAAHKGWGESIIIGVAPAGAEIATR
CCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCC
PFQLVTGRVWRGTAFGGAKGRTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMHQ
CEEHHCCEEEECEECCCCCCCCCCHHHHHHHCCCEEEECCEEEEEECHHHHCCCHHHHHC
GKSIRAVVEF
CCCEEEEEEC
>Mature Secondary Structure
MRTRAAVAVAAGQPLEVMEVNLDGPRAGEVLVEIKATGLCHTDEFTRSGDDPEGIFPAIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCCEECCHHHCCCCCCCCCCHHHH
GHEGAGVVIEVGEGVTSLEVGDHVIPLYTPECRECDYCLNPKTNLCQKVRATQGAGLLPD
CCCCCEEEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GSTRFSMLDGTPIHHYMGCSTFANHTVVPEIALAKVRKDAPFDKICYIGCGVTTGIGAVI
CCCEEEEECCCCHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHE
NTAKVEIGSTAAVFGLGGIGLNVIQGLRMAGADQIIGVDLNDDKEKMARHFGMTDFVNPS
EEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCC
KVEGDMVAHLVELSGGGCDYTFDATGNVNVMRTALEAAHKGWGESIIIGVAPAGAEIATR
CCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCC
PFQLVTGRVWRGTAFGGAKGRTDVPKIVDWYMDGKIEIDPMITHKLSLDEINHGFDLMHQ
CEEHHCCEEEECEECCCCCCCCCCHHHHHHHCCCEEEECCEEEEEECHHHHCCCHHHHHC
GKSIRAVVEF
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]