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The map label for this gene is nifS [H]

Identifier: 110678159

GI number: 110678159

Start: 779429

End: 780592

Strand: Direct

Name: nifS [H]

Synonym: RD1_0802

Alternate gene names: 110678159

Gene position: 779429-780592 (Clockwise)

Preceding gene: 110678158

Following gene: 110678160

Centisome position: 18.86

GC content: 54.98

Gene sequence:

>1164_bases
ATGTCGGCACCGATTTACCTTGACCACAACGCTTCTACTCCGATTGATCCGGAAGTTTTGGAGACGGTCATACGTGTCAG
TCGGGACGTATATGCGAACCCATCAAGTGTTGAGCATAGCCAAGGTTCAGGGGCTGCTCGGGTTGTCGATACTGCGCGCG
CGCAGATTGCGGGCCATCTCAATTGCAAGGAAACCGAGCTCGTCTTTACAGCCGGGTCCACGGAAGCCAACAATCTTGCG
ATCCTTGGAGCGTTCCCTCGGCTCAAGGAGGCGGGGCGCACACATCTGATCACCAGCAAGATCGAGCACCCATCTGTTCT
GGCCTGTTTCGACCATCTCGAATCCCAAGGCGCACGGGTAACGCGATTGGGGGTAGATGAAGGTGGCCAGATTTCCCTCG
ATGAGCTGGCCCTGTCTTTGACGGATGAGACCGGATTCTTGTCCATCATGGCTGCCAACAATGAGACGGGCGTTTTGCAA
CCCATCTTGGAAGCAGGCCAGCTTGCCGAAGAGGCTGGGGCGCTATTTCACACGGACTTCTCGCAAGCTTCGGCTTTTGT
GCCACTTGATCTCAAGAACGCGCCCATCCATCTGGCGAGTTTCTCTGGCCACAAGGCCTATGGCCCGAAAGGGGTTGGGG
CCCTTTACCGGTCGATCCGAAAACCGCGGGTCAACCTAGCCCCAGTGATGTTTGGTGGCGGGCAGGAGAAGGGTTTGAGG
GCAGGGACCCTGAACACGCCTGGCATTGCCGGCCTTGGACATGCATTTGAGCTCATCTCAAAAAACATAGACCATGACCG
GGATCGGATTGGGGCCCTGCGAGATCAGCTTGAAATCGAACTCAAAGAGACCTTAGCGGCGCAGGTCAATGGAAACACTG
CAGCGAGGTTGCCAAACACGATGTCCCTCACGATCGACGGAGTTGTCCCACAAGCGCTGATGCAAAAGCTAAGAAACGAC
GTCTGCTTTTCTGCATCGAGCGCCTGCGCCACAGAACATGTCGAAACCTCTCACGTTCTGCTCGCCATGTTCGGCGACAC
TCCAAGGGCGCGAAATGCTTTCCGACTAGGTCTAGGCCGGCAAACCAGCAAAAAAGACATATCCAAAGTCATCAGACTCT
TTTTACAAGCCTCATCAGAACTCCTGCTTCTAAAAACAGCTTAG

Upstream 100 bases:

>100_bases
CGCTTGCGATTGCACATTGATCGTGGTGTGCGGCAACTCTCGGTCCGGCTTAAATCCCCGGCGGATGCGGCCGAACTGAT
CGCGGGGAGTGCGTGAAGCG

Downstream 100 bases:

>100_bases
AAACCAGCCGATCCGCCACAATTTCGAGAGGTACAGGTAGGATAGCAATAAAGGCTACGAACTTCCATGGATGCTGAGCG
AAAGCAGACTTACAAGACAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSAPIYLDHNASTPIDPEVLETVIRVSRDVYANPSSVEHSQGSGAARVVDTARAQIAGHLNCKETELVFTAGSTEANNLA
ILGAFPRLKEAGRTHLITSKIEHPSVLACFDHLESQGARVTRLGVDEGGQISLDELALSLTDETGFLSIMAANNETGVLQ
PILEAGQLAEEAGALFHTDFSQASAFVPLDLKNAPIHLASFSGHKAYGPKGVGALYRSIRKPRVNLAPVMFGGGQEKGLR
AGTLNTPGIAGLGHAFELISKNIDHDRDRIGALRDQLEIELKETLAAQVNGNTAARLPNTMSLTIDGVVPQALMQKLRND
VCFSASSACATEHVETSHVLLAMFGDTPRARNAFRLGLGRQTSKKDISKVIRLFLQASSELLLLKTA

Sequences:

>Translated_387_residues
MSAPIYLDHNASTPIDPEVLETVIRVSRDVYANPSSVEHSQGSGAARVVDTARAQIAGHLNCKETELVFTAGSTEANNLA
ILGAFPRLKEAGRTHLITSKIEHPSVLACFDHLESQGARVTRLGVDEGGQISLDELALSLTDETGFLSIMAANNETGVLQ
PILEAGQLAEEAGALFHTDFSQASAFVPLDLKNAPIHLASFSGHKAYGPKGVGALYRSIRKPRVNLAPVMFGGGQEKGLR
AGTLNTPGIAGLGHAFELISKNIDHDRDRIGALRDQLEIELKETLAAQVNGNTAARLPNTMSLTIDGVVPQALMQKLRND
VCFSASSACATEHVETSHVLLAMFGDTPRARNAFRLGLGRQTSKKDISKVIRLFLQASSELLLLKTA
>Mature_386_residues
SAPIYLDHNASTPIDPEVLETVIRVSRDVYANPSSVEHSQGSGAARVVDTARAQIAGHLNCKETELVFTAGSTEANNLAI
LGAFPRLKEAGRTHLITSKIEHPSVLACFDHLESQGARVTRLGVDEGGQISLDELALSLTDETGFLSIMAANNETGVLQP
ILEAGQLAEEAGALFHTDFSQASAFVPLDLKNAPIHLASFSGHKAYGPKGVGALYRSIRKPRVNLAPVMFGGGQEKGLRA
GTLNTPGIAGLGHAFELISKNIDHDRDRIGALRDQLEIELKETLAAQVNGNTAARLPNTMSLTIDGVVPQALMQKLRNDV
CFSASSACATEHVETSHVLLAMFGDTPRARNAFRLGLGRQTSKKDISKVIRLFLQASSELLLLKTA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=379, Percent_Identity=37.9947229551451, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=34.5410628019324, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=38.6422976501305, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1787970, Length=273, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789175, Length=306, Percent_Identity=28.4313725490196, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=379, Percent_Identity=38.2585751978892, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.0987654320988, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=39.5663956639566, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI20129463, Length=365, Percent_Identity=40.2739726027397, Blast_Score=261, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41326; Mature: 41194

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPIYLDHNASTPIDPEVLETVIRVSRDVYANPSSVEHSQGSGAARVVDTARAQIAGHL
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NCKETELVFTAGSTEANNLAILGAFPRLKEAGRTHLITSKIEHPSVLACFDHLESQGARV
CCCCEEEEEEECCCCCCCEEEEECCHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEE
TRLGVDEGGQISLDELALSLTDETGFLSIMAANNETGVLQPILEAGQLAEEAGALFHTDF
EEECCCCCCCEEHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECH
SQASAFVPLDLKNAPIHLASFSGHKAYGPKGVGALYRSIRKPRVNLAPVMFGGGQEKGLR
HHCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
AGTLNTPGIAGLGHAFELISKNIDHDRDRIGALRDQLEIELKETLAAQVNGNTAARLPNT
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCE
MSLTIDGVVPQALMQKLRNDVCFSASSACATEHVETSHVLLAMFGDTPRARNAFRLGLGR
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCC
QTSKKDISKVIRLFLQASSELLLLKTA
CCCHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
SAPIYLDHNASTPIDPEVLETVIRVSRDVYANPSSVEHSQGSGAARVVDTARAQIAGHL
CCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NCKETELVFTAGSTEANNLAILGAFPRLKEAGRTHLITSKIEHPSVLACFDHLESQGARV
CCCCEEEEEEECCCCCCCEEEEECCHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEE
TRLGVDEGGQISLDELALSLTDETGFLSIMAANNETGVLQPILEAGQLAEEAGALFHTDF
EEECCCCCCCEEHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECH
SQASAFVPLDLKNAPIHLASFSGHKAYGPKGVGALYRSIRKPRVNLAPVMFGGGQEKGLR
HHCCEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
AGTLNTPGIAGLGHAFELISKNIDHDRDRIGALRDQLEIELKETLAAQVNGNTAARLPNT
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCE
MSLTIDGVVPQALMQKLRNDVCFSASSACATEHVETSHVLLAMFGDTPRARNAFRLGLGR
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCCEEECCCC
QTSKKDISKVIRLFLQASSELLLLKTA
CCCHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA