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The map label for this gene is kefA [C]

Identifier: 110677997

GI number: 110677997

Start: 600528

End: 601844

Strand: Direct

Name: kefA [C]

Synonym: RD1_0618

Alternate gene names: 110677997

Gene position: 600528-601844 (Clockwise)

Preceding gene: 110677993

Following gene: 110677998

Centisome position: 14.53

GC content: 58.77

Gene sequence:

>1317_bases
ATGGAAAACCAGTCGCAGGACTTTGATCGCGTTCTGATGTCAGTCGATGAGATCTGGCATCTGATCACCAGCTTTGGCGT
CAGCATTATGAATCCGGGCTGGCGTCAAAACCAGATCATCATCCTGCTGGCACTTGTGGTTGTGGCCTGGGTCCTGCACC
GCCTTTTGGGCCGCGAAATCGACCGCCGCGTGCGGGCGCGCGAGGGGTGGGAGAAATGGAAACTCCGGGTCATTGTTCAG
ATCAAGCGCCGTCTGGGCCTGATCTGTTTTGCGCTGATGGCCGGTCTTTTGTATCAGATCATGCAGCAGATCACCTGGCC
CTCGCGCTCCTATTTGATCGGTCTGGCTGCAACGCTGGCGGTCGTCTGGGCGGTGATCAGTTTTCTCGGACGTTTTATCC
AGAACCGTTTGCTGCGCCGGTTTGTGGTCTGGGGGATGTGGATCTATGCGACGCTGATCCTGATCGGCCTTGGCGATGAG
GCATCGGGGTTTCTGGATGATGTCGGTCTGAACTTTGGCGATTACCGTATTTCACTGCTTTCCGTGCTCTCTGCGGCCGT
GGTGGTTGGCGTGCTGTTCACCGGCGCGCGGTTCATCAGCCGCACCACCGCATCGCGCATCCGCAGCAACGAAGACATCA
GCCCCTCCATGCAGGTGCTTGTGGTCAAGGCGGTGCAGGTCATCTTCTACGGGCTTGCGTTTTTCATGGGGATCAATGCG
CTGGGTCTGGACCTCACCGGGCTGGCGGTGCTGTCCGGTGCCATTGGTGTGGGGCTGGGGTTCGGTCTGCAAAAGGTCGT
CTCCAACCTCGTGTCGGGTGTGATCATCCTGCTCGACAAGTCGATCAAGCCCGGCGATGTGATCAGCCTTGGCGAAACAT
TCGGCTGGATCAACAGTCTGGGCGCGCGCTACGTCAGCGTGGTGACACGGGACGGGCGCGAATACTTGATCCCGAATGAG
GATCTGATCACCGGGCAGGTGGTGAACTGGTCCCACTCGGATGAATATGTCCGGCTCGATATCTTTTTCGGCACCTCCTA
CGGCTGCGATCCGCATGTGGTGCGCAAACTGGCGGTCGAGGCGGCCAGCGGCGTGGCCCGTGTTCTGGGGCCACCCAAGA
CGCCCGTGTGTCATATCGTCGGGTTTGGCGACAGTTCGGTTGATTACATTCTCAGGTTCTGGATCAGGGACCCGACCGGC
GGGCTCACCAACATCCGCGGCGGGGTTTATCTGGCGCTCTGGGATATTTTCAAGGCGAACAACATCTCCATCCCCTTCCC
TCAGCGCGAGGTTCGGGTTCTTGAGGAACAGGATTAA

Upstream 100 bases:

>100_bases
TCATTGCGTCGAACCGATAGCGTGAAATGCGCGCTTCATCAAGCCTTGGGAATTGCACATCGCCTTGTGAGTGTTTGGCC
GATATGTAACCCTGCCCCTT

Downstream 100 bases:

>100_bases
GGCAAGACCGTCTTGCCACCGCCTGATCCGGGGCTAACTTCTGCACTTCCCAACGGCAGACGGCGACGGTTATGGCGCGA
CAGACAGACATCGTATTGAT

Product: mechanosensitive ion channel protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MENQSQDFDRVLMSVDEIWHLITSFGVSIMNPGWRQNQIIILLALVVVAWVLHRLLGREIDRRVRAREGWEKWKLRVIVQ
IKRRLGLICFALMAGLLYQIMQQITWPSRSYLIGLAATLAVVWAVISFLGRFIQNRLLRRFVVWGMWIYATLILIGLGDE
ASGFLDDVGLNFGDYRISLLSVLSAAVVVGVLFTGARFISRTTASRIRSNEDISPSMQVLVVKAVQVIFYGLAFFMGINA
LGLDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSVVTRDGREYLIPNE
DLITGQVVNWSHSDEYVRLDIFFGTSYGCDPHVVRKLAVEAASGVARVLGPPKTPVCHIVGFGDSSVDYILRFWIRDPTG
GLTNIRGGVYLALWDIFKANNISIPFPQREVRVLEEQD

Sequences:

>Translated_438_residues
MENQSQDFDRVLMSVDEIWHLITSFGVSIMNPGWRQNQIIILLALVVVAWVLHRLLGREIDRRVRAREGWEKWKLRVIVQ
IKRRLGLICFALMAGLLYQIMQQITWPSRSYLIGLAATLAVVWAVISFLGRFIQNRLLRRFVVWGMWIYATLILIGLGDE
ASGFLDDVGLNFGDYRISLLSVLSAAVVVGVLFTGARFISRTTASRIRSNEDISPSMQVLVVKAVQVIFYGLAFFMGINA
LGLDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSVVTRDGREYLIPNE
DLITGQVVNWSHSDEYVRLDIFFGTSYGCDPHVVRKLAVEAASGVARVLGPPKTPVCHIVGFGDSSVDYILRFWIRDPTG
GLTNIRGGVYLALWDIFKANNISIPFPQREVRVLEEQD
>Mature_438_residues
MENQSQDFDRVLMSVDEIWHLITSFGVSIMNPGWRQNQIIILLALVVVAWVLHRLLGREIDRRVRAREGWEKWKLRVIVQ
IKRRLGLICFALMAGLLYQIMQQITWPSRSYLIGLAATLAVVWAVISFLGRFIQNRLLRRFVVWGMWIYATLILIGLGDE
ASGFLDDVGLNFGDYRISLLSVLSAAVVVGVLFTGARFISRTTASRIRSNEDISPSMQVLVVKAVQVIFYGLAFFMGINA
LGLDLTGLAVLSGAIGVGLGFGLQKVVSNLVSGVIILLDKSIKPGDVISLGETFGWINSLGARYVSVVTRDGREYLIPNE
DLITGQVVNWSHSDEYVRLDIFFGTSYGCDPHVVRKLAVEAASGVARVLGPPKTPVCHIVGFGDSSVDYILRFWIRDPTG
GLTNIRGGVYLALWDIFKANNISIPFPQREVRVLEEQD

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=198, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI2367355, Length=222, Percent_Identity=26.5765765765766, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789291, Length=270, Percent_Identity=28.1481481481481, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48728; Mature: 48728

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHCCCCC
>Mature Secondary Structure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CCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]