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The map label for this gene is bphF [H]

Identifier: 110677707

GI number: 110677707

Start: 293443

End: 294213

Strand: Direct

Name: bphF [H]

Synonym: RD1_0304

Alternate gene names: 110677707

Gene position: 293443-294213 (Clockwise)

Preceding gene: 110677706

Following gene: 110677709

Centisome position: 7.1

GC content: 59.53

Gene sequence:

>771_bases
ATGCCCGCGCCACTGAACACGTTCAAAAAATCCCTGTCCGAAGGGGAGACGCTTTTTGGCTGCTGGATGAGCATCGCAGA
GAGCTATACCGCAGAGATATTGGGCAATGCGGGCTTTGACTGGCTGCTGATTGATGGCGAGCATTCGCCCAATGACGTGC
GTTCGATCCGCGACCAGATGATCGCGCTGAAAAGCAGTGACAGCCACCCGATCATCCGCGTGCCGGTGGGCGAGACGTGG
ATCATCAAGCAAATGCTGGACCTTGGGGCCCAGACGATTCTCGTGCCCATGGTGGAAACCGCCGAGCAGGCGCAGGAACT
CGTGCGCGCCTGCCGATACCCGCCGGAGGGCAGGCGTGGCGTGGGCTATGCGGTGGGGCGTGCGTCAGGCTTTGGCCAGA
TCGACAACTACGGTCCGACCGCGGATGCACAGACGTGCCTGCTGGTGCAGGTTGAGAATAAAACTGGCCTTTATAACCTT
GACGCGATCCTTGCGGTCGATGGCGTTGACGGTGTGTTCATTGGCCCGGCGGATCTGTCTGCAAGCCTCGGATACCTCGG
GCAGACCATGCACCCGGACATACAGGCGACAATCCTGAAAACCATCAAGCGGATCAGCGACAGCGGCAAGGCCGCCGGTA
TCCTGACCACGGATGACGGTATGATCCAAGCGTCCTTGGATGCCGGTGCCCGGTTTGTTGCCGTGGCCATGGACATCGCG
ATGCTTTTGAACACCGGGAAATCCATCGCCGCCAAGTGGAAACAGGGTTAA

Upstream 100 bases:

>100_bases
GGACGTCATCAAAGTCATCTGAAACCGCGCACTGCACTGGTGTTTCACGGCGTGAGCGTCCAAAACTGACCGCGCAGAGA
CCCCAAAGAGGAAACCAGAC

Downstream 100 bases:

>100_bases
CGCGTCTTCATCTGCGCGGCCGCCTGCGTATATCCCCCCGCTGCCCCATCAATGAAATGCAGATGGTCGTCGGTAAGAAT
CGACGGAACGATGCAGGTCA

Product: 4-hydroxy-2-oxovalerate aldolase

Products: NA

Alternate protein names: KDR aldolase; 2-dehydro-3-deoxyrhamnonate aldolase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MPAPLNTFKKSLSEGETLFGCWMSIAESYTAEILGNAGFDWLLIDGEHSPNDVRSIRDQMIALKSSDSHPIIRVPVGETW
IIKQMLDLGAQTILVPMVETAEQAQELVRACRYPPEGRRGVGYAVGRASGFGQIDNYGPTADAQTCLLVQVENKTGLYNL
DAILAVDGVDGVFIGPADLSASLGYLGQTMHPDIQATILKTIKRISDSGKAAGILTTDDGMIQASLDAGARFVAVAMDIA
MLLNTGKSIAAKWKQG

Sequences:

>Translated_256_residues
MPAPLNTFKKSLSEGETLFGCWMSIAESYTAEILGNAGFDWLLIDGEHSPNDVRSIRDQMIALKSSDSHPIIRVPVGETW
IIKQMLDLGAQTILVPMVETAEQAQELVRACRYPPEGRRGVGYAVGRASGFGQIDNYGPTADAQTCLLVQVENKTGLYNL
DAILAVDGVDGVFIGPADLSASLGYLGQTMHPDIQATILKTIKRISDSGKAAGILTTDDGMIQASLDAGARFVAVAMDIA
MLLNTGKSIAAKWKQG
>Mature_255_residues
PAPLNTFKKSLSEGETLFGCWMSIAESYTAEILGNAGFDWLLIDGEHSPNDVRSIRDQMIALKSSDSHPIIRVPVGETWI
IKQMLDLGAQTILVPMVETAEQAQELVRACRYPPEGRRGVGYAVGRASGFGQIDNYGPTADAQTCLLVQVENKTGLYNLD
AILAVDGVDGVFIGPADLSASLGYLGQTMHPDIQATILKTIKRISDSGKAAGILTTDDGMIQASLDAGARFVAVAMDIAM
LLNTGKSIAAKWKQG

Specific function: Catalyzes the reversible retro-aldol cleavage of 2-keto- 3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=251, Percent_Identity=50.597609561753, Blast_Score=250, Evalue=6e-68,
Organism=Escherichia coli, GI1789514, Length=250, Percent_Identity=43.2, Blast_Score=227, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27358; Mature: 27226

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPLNTFKKSLSEGETLFGCWMSIAESYTAEILGNAGFDWLLIDGEHSPNDVRSIRDQM
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
IALKSSDSHPIIRVPVGETWIIKQMLDLGAQTILVPMVETAEQAQELVRACRYPPEGRRG
EEEECCCCCCEEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
VGYAVGRASGFGQIDNYGPTADAQTCLLVQVENKTGLYNLDAILAVDGVDGVFIGPADLS
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECEEEEEECCCCEEEECCHHHH
ASLGYLGQTMHPDIQATILKTIKRISDSGKAAGILTTDDGMIQASLDAGARFVAVAMDIA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHH
MLLNTGKSIAAKWKQG
HHHHCCHHHHHCCCCC
>Mature Secondary Structure 
PAPLNTFKKSLSEGETLFGCWMSIAESYTAEILGNAGFDWLLIDGEHSPNDVRSIRDQM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
IALKSSDSHPIIRVPVGETWIIKQMLDLGAQTILVPMVETAEQAQELVRACRYPPEGRRG
EEEECCCCCCEEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
VGYAVGRASGFGQIDNYGPTADAQTCLLVQVENKTGLYNLDAILAVDGVDGVFIGPADLS
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECEEEEEECCCCEEEECCHHHH
ASLGYLGQTMHPDIQATILKTIKRISDSGKAAGILTTDDGMIQASLDAGARFVAVAMDIA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHH
MLLNTGKSIAAKWKQG
HHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA