BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

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The map label for this gene is fadB1 [H]

Identifier: 110677613

GI number: 110677613

Start: 202803

End: 203579

Strand: Direct

Name: fadB1 [H]

Synonym: RD1_0205

Alternate gene names: 110677613

Gene position: 202803-203579 (Clockwise)

Preceding gene: 110677612

Following gene: 110677614

Centisome position: 4.91

GC content: 55.47

Gene sequence:

>777_bases
ATGGCTTATGAAACGATCATCGTAGACGTAGAAGACCACGTCGCACAGATCACACTGAACCGTCCCGATGCGCTCAATGC
GCTGAATGATGAGTTGATGAGCGAATTGGCAAATGCGCTCACAGCTGCACAGAATAACGACAAGGTCCGCTGCATAATCC
TGACCGGATCAGAAAAAGCCTTCGCGGCGGGCGCTGATATCTCGATGATGAAGGACAAGAGCTTTGTCGAAGTGTTCTCG
GGTGATTTGTTCACCGCAGAGACAGAAGAGATCATGCGCGTGCGCAAGCCGATCATTGCCGCTGTTTCAGGCTATGCTTT
GGGTGGTGGGTGCGAATTGGCGATGATGTGTGATTTCATCATTTGTTCAGAGACGGCCAAGTTCGGACAACCCGAGATCA
ACCTCGGCGTGGTGGCTGGCCTTGGCGGCACGCAGCGGCTGACCCGTCTGGTGGGCAAGTCAAAAGCGATGGATATGAAT
CTGACCGGTCGCTTCATGGACGCCGAAGAGGCAGAGCGTTCCGGATTGGTAAGCCGCGTCGTGCCCTGCAAGAAACTGAT
GGATGAAGCACATGGCGCGGCACAGCGCATCGCGGAAAAATCCATGGTGTCGGTCATGGTCGTCAAGGAAGCCGTGAACC
GGGCCTATGAAACGACCCTGCGCGAAGGTCTGCTGTTTGAGCGGCGCATGTTCCACTCGCTGTTTGCGACCGAGGATCAG
AAAGAGGGCATGTCCGCCTTTGTCGAAAAGCGCGAAGCACAGTTCCGCGACCGCTGA

Upstream 100 bases:

>100_bases
CGCTCATCTTTCTATTGCGCGCAATGCCAAAGATAGCTTGAACCAGCCCAATGGAGTGGTAGAGACTGCGCATTCAGGTC
AACAACAAAGAGCTTTCTTC

Downstream 100 bases:

>100_bases
TTACAGAAATAGCGAAGGACACAGCACGATGGCTCACCCTATCAAACATGCGATGGCTGCGACCTTGCTGGCTCTGGGCG
GGGCTGCGCAGGCGGAAATG

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYETIIVDVEDHVAQITLNRPDALNALNDELMSELANALTAAQNNDKVRCIILTGSEKAFAAGADISMMKDKSFVEVFS
GDLFTAETEEIMRVRKPIIAAVSGYALGGGCELAMMCDFIICSETAKFGQPEINLGVVAGLGGTQRLTRLVGKSKAMDMN
LTGRFMDAEEAERSGLVSRVVPCKKLMDEAHGAAQRIAEKSMVSVMVVKEAVNRAYETTLREGLLFERRMFHSLFATEDQ
KEGMSAFVEKREAQFRDR

Sequences:

>Translated_258_residues
MAYETIIVDVEDHVAQITLNRPDALNALNDELMSELANALTAAQNNDKVRCIILTGSEKAFAAGADISMMKDKSFVEVFS
GDLFTAETEEIMRVRKPIIAAVSGYALGGGCELAMMCDFIICSETAKFGQPEINLGVVAGLGGTQRLTRLVGKSKAMDMN
LTGRFMDAEEAERSGLVSRVVPCKKLMDEAHGAAQRIAEKSMVSVMVVKEAVNRAYETTLREGLLFERRMFHSLFATEDQ
KEGMSAFVEKREAQFRDR
>Mature_257_residues
AYETIIVDVEDHVAQITLNRPDALNALNDELMSELANALTAAQNNDKVRCIILTGSEKAFAAGADISMMKDKSFVEVFSG
DLFTAETEEIMRVRKPIIAAVSGYALGGGCELAMMCDFIICSETAKFGQPEINLGVVAGLGGTQRLTRLVGKSKAMDMNL
TGRFMDAEEAERSGLVSRVVPCKKLMDEAHGAAQRIAEKSMVSVMVVKEAVNRAYETTLREGLLFERRMFHSLFATEDQK
EGMSAFVEKREAQFRDR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=51.3513513513513, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI20127408, Length=181, Percent_Identity=38.121546961326, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI68989263, Length=232, Percent_Identity=31.8965517241379, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=34.6774193548387, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=29.6, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4757968, Length=191, Percent_Identity=30.3664921465969, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI49355787, Length=191, Percent_Identity=30.3664921465969, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=26.5765765765766, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI45643119, Length=247, Percent_Identity=25.9109311740891, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI260275230, Length=247, Percent_Identity=25.9109311740891, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI25453481, Length=190, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI51538573, Length=190, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=243, Percent_Identity=25.9259259259259, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=243, Percent_Identity=25.9259259259259, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=27.3584905660377, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=27.3584905660377, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221136756, Length=184, Percent_Identity=28.804347826087, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136753, Length=184, Percent_Identity=28.804347826087, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221307494, Length=184, Percent_Identity=28.804347826087, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI157694520, Length=215, Percent_Identity=25.1162790697674, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=26.046511627907, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=42.6877470355731, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=36, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=34.469696969697, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=33.4615384615385, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=34, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788682, Length=225, Percent_Identity=34.6666666666667, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=52.5896414342629, Blast_Score=266, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=51.6393442622951, Blast_Score=248, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=31.5789473684211, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=238, Percent_Identity=31.5126050420168, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=30.8370044052863, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=266, Percent_Identity=28.9473684210526, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=31.2, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=32.967032967033, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=241, Percent_Identity=29.4605809128631, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=241, Percent_Identity=29.4605809128631, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=33.8541666666667, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=33.8541666666667, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=255, Percent_Identity=26.2745098039216, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=22.7450980392157, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=30.5699481865285, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=55.8139534883721, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=55.8139534883721, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=28.2352941176471, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=31.496062992126, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19920382, Length=231, Percent_Identity=32.034632034632, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650670, Length=214, Percent_Identity=33.1775700934579, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357171, Length=248, Percent_Identity=28.2258064516129, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=28.1914893617021, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19921000, Length=206, Percent_Identity=33.495145631068, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583077, Length=206, Percent_Identity=33.495145631068, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583079, Length=206, Percent_Identity=33.495145631068, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571729, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28571730, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583165, Length=176, Percent_Identity=30.6818181818182, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921018, Length=190, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28364; Mature: 28233

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETIIVDVEDHVAQITLNRPDALNALNDELMSELANALTAAQNNDKVRCIILTGSEKA
CCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADISMMKDKSFVEVFSGDLFTAETEEIMRVRKPIIAAVSGYALGGGCELAMMCDFI
EECCCCHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
ICSETAKFGQPEINLGVVAGLGGTQRLTRLVGKSKAMDMNLTGRFMDAEEAERSGLVSRV
HHHCHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPCKKLMDEAHGAAQRIAEKSMVSVMVVKEAVNRAYETTLREGLLFERRMFHSLFATEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KEGMSAFVEKREAQFRDR
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AYETIIVDVEDHVAQITLNRPDALNALNDELMSELANALTAAQNNDKVRCIILTGSEKA
CCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FAAGADISMMKDKSFVEVFSGDLFTAETEEIMRVRKPIIAAVSGYALGGGCELAMMCDFI
EECCCCHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
ICSETAKFGQPEINLGVVAGLGGTQRLTRLVGKSKAMDMNLTGRFMDAEEAERSGLVSRV
HHHCHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPCKKLMDEAHGAAQRIAEKSMVSVMVVKEAVNRAYETTLREGLLFERRMFHSLFATEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KEGMSAFVEKREAQFRDR
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]