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The map label for this gene is bcsZ [H]

Identifier: 162139423

GI number: 162139423

Start: 4181824

End: 4182744

Strand: Reverse

Name: bcsZ [H]

Synonym: YPN_3647

Alternate gene names: 162139423

Gene position: 4182744-4181824 (Counterclockwise)

Preceding gene: 108813808

Following gene: 108813806

Centisome position: 92.24

GC content: 50.27

Gene sequence:

>921_bases
TTGGTTGCGGATGATCAGCCGATGTTTGAGCGTTTATTGGCTTGGACAGAAAACAATCTGGCAGCCGGTGATCTCACTTC
CCGCCTTCCCGCTTGGTTATGGGGGCAAAACTCACAAAATAACTGGGATATTCTGGACCCTAATTCGGCCTCCGATGCGG
ATATCTTGATTGCCTACAACTTGCTGGAGGCTGGCAGGTTATGGGGTAACCGCCGTTACCTGATTATGGGTACCTTATTA
CTGCAACGTATTGCGCAAGAAGAAGTCATGGATATTCCCGGCCTTGGCCAGATGCTATTACCGGGAAAAATTGGTTTTAA
CGATGAGGATACCTGGCGTCTCAACCCAAGTTATTTACCGCCACAACTACTGGCACGATTTTCCTCCATAGACGGGCCTT
GGGAAGCGATGGTAGAAGTGAATCAGCGTATGTGGCTGGAAACCGCACCAAACGGTTTTTCGCTGGACTGGGTGGTCTGG
CAGAAAGGTAAAGGCTGGCAGCCCGATACCATAAAACCGGATGTCGGCAGTAACGATGCCATTCTGGTTTATCTGTGGGC
GGGGATGCTGGCAATGGACAGCCCACAAAAAGCTGAATTGATTGCGCGTTTTCAGCCAATGGCGGTAATCACTCAGCAGC
AAGGCCTGCCACCGTTTACGACCAACAGCGACAATGGTAAAACTAACGGGGATGGGGCAGTGGGTTTTTCTGCGGCATTA
TTGCCCTTTTTAGCCAGCAGCCCAGAACCGTTTAATCAGCAAACACTGAATCTCCAACAGCGACGGGTACAAAATTCACC
GCCTGGCGCTGATGATTATTACAGTGCTATTCTGACCCTGTTTGGTCAGGGGTGGTTACAGCATCGTTATCATTTTACCC
ATCAGGGAGAGCTACAGCCCTCATGGCACCGTCAACGTTAA

Upstream 100 bases:

>100_bases
TCAAACAAGATTACATCAGTGAAGGGGGGCGGATCATTGACCCCGGTAGCCCCTCGAAAATAACCACCTCGGAAGGCCAG
AGTTATGGGCTTTTTTTGCA

Downstream 100 bases:

>100_bases
GATTTCAGGATAGCGGATCTGGCCTAATGATAGGGAAATGCGCTTAAGGGATCACAACTTACTCGATGGGGACTAAAAAG
GGGCGTCTAAACACCAAGCA

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLL
LQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSLDWVVW
QKGKGWQPDTIKPDVGSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGAVGFSAAL
LPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR

Sequences:

>Translated_306_residues
MVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLL
LQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSLDWVVW
QKGKGWQPDTIKPDVGSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGAVGFSAAL
LPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR
>Mature_306_residues
MVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLL
LQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSLDWVVW
QKGKGWQPDTIKPDVGSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGAVGFSAAL
LPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=305, Percent_Identity=51.4754098360656, Blast_Score=334, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 34520; Mature: 34520

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYN
CCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCEECCCCCCCCEEEEEEH
LLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLNPSYLP
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEECCCCCC
PQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSLDWVVWQKGKGWQPDTIKPDVGSNDA
HHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
ILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGAVGFSAAL
EEEEEEHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQP
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
SWHRQR
CCCCCC
>Mature Secondary Structure
MVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYN
CCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCEECCCCCCCCEEEEEEH
LLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLNPSYLP
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEECCCCCC
PQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSLDWVVWQKGKGWQPDTIKPDVGSNDA
HHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
ILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGAVGFSAAL
EEEEEEHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQP
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
SWHRQR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]