Click here to switch to the map view.

The map label for this gene is insK [C]

Identifier: 108813582

GI number: 108813582

Start: 3899795

End: 3900550

Strand: Reverse

Name: insK [C]

Synonym: YPN_3422

Alternate gene names: 108813582

Gene position: 3900550-3899795 (Counterclockwise)

Preceding gene: 108813583

Following gene: 108813579

Centisome position: 86.02

GC content: 49.34

Gene sequence:

>756_bases
ATGATGCAGAGCCGCACTGTATACAACTATCGTAGCCATCGTGACGATCGGGCTATTACTCAGCGAATACGGGAAATTGC
GGAGACGCGCATCCGTTATGGCTGTCCGCGCATTCATATCCTGCTGCGGAGAGAAGGCTGGCTTGTTAACCATAAGAAAA
CGCACCGTATTTACTGTCTTGAGGGTCTCAATCTACGTTCGAAACGTCCACGGTGGCATGTGACAGCGAGGCACAGGCAC
GCAAGTCCAGAAGTGACCGCGTTAGATCAGTGCTGGAGCATGGATTTCGTTGCTGATAATCTGTTCAACGGGCGTCGGGT
CAGGGCGCTAACTATAGTGGATAATTTTAGTCGTGAATGTCTGGCGATCGAGGTCGGTCAGGGGTTACGTGGAGATGATG
TTGTGGCTGTCATGGACAGATTAAAACATTCGCTGGGGCGTATTCCACAAAGGCTGCAGACAGATAACGGCAGCGAATTC
ATCTCGAAGTCGATGGACCGATGGGCGTATGAAAACAGGGTCTCGATGGACTTTTCAGGCCCCGGAAAGCCTACAGATAA
TGCCTTTATCGAGTCATTTAATGGCAGTCTGAGGGATGAATGTCTGAACGTGCACGGGTTCCTTTCTCTGGAAGATGCTC
AGGAGAAAATTGAACAATGGCGGCAAGAATATAATCATTTTCGTCCGTATTCTTCGTTAAATAACCTGACTCCGGCAGAA
TTTGCCCGAAGTCATCAAAAAGGTCCGGATCTCTGA

Upstream 100 bases:

>100_bases
ATGTCATCCGAAAAAAGTTCTGAGGCCGCTGCAGAAGCGCGATGCCGTGGAATATCTGCTGGCGGCGTACCGTATCGGAG
TACGCAGAGGGTGCAGGCTG

Downstream 100 bases:

>100_bases
TTTAGCCTGGTACGGATATTGGGTATTGGGGAGGGATCATCCTCTCCTTATTATTTTGTTCGTCGCGGCCCTTGAGTGGG
GATACTGTGGGTATAATCGA

Product: transposase

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRWHVTARHRH
ASPEVTALDQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEF
ISKSMDRWAYENRVSMDFSGPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPYSSLNNLTPAE
FARSHQKGPDL

Sequences:

>Translated_251_residues
MMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRWHVTARHRH
ASPEVTALDQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEF
ISKSMDRWAYENRVSMDFSGPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPYSSLNNLTPAE
FARSHQKGPDL
>Mature_251_residues
MMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRWHVTARHRH
ASPEVTALDQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEF
ISKSMDRWAYENRVSMDFSGPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPYSSLNNLTPAE
FARSHQKGPDL

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=235, Percent_Identity=25.9574468085106, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786462, Length=243, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790724, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789424, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789224, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1788305, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1787669, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1786558, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29411; Mature: 29411

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCEEEEE
EGLNLRSKRPRWHVTARHRHASPEVTALDQCWSMDFVADNLFNGRRVRALTIVDNFSREC
ECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCE
LAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVSMDFSG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCC
PGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPYSSLNNLTPAE
CCCCCCHHHHHHHCCHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHH
FARSHQKGPDL
HHHHCCCCCCC
>Mature Secondary Structure
MMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCCEEEEE
EGLNLRSKRPRWHVTARHRHASPEVTALDQCWSMDFVADNLFNGRRVRALTIVDNFSREC
ECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCE
LAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVSMDFSG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCC
PGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPYSSLNNLTPAE
CCCCCCHHHHHHHCCHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHH
FARSHQKGPDL
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]