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The map label for this gene is thiE [H]

Identifier: 108810392

GI number: 108810392

Start: 280391

End: 281065

Strand: Reverse

Name: thiE [H]

Synonym: YPN_0226

Alternate gene names: 108810392

Gene position: 281065-280391 (Counterclockwise)

Preceding gene: 108810393

Following gene: 108810391

Centisome position: 6.2

GC content: 53.33

Gene sequence:

>675_bases
ATGAGCCAACTTGATGCCCTCTCTCCTGTGGCTACACCAGGTTTCCCCTCCACGGAACAGCGTCTTGGGCTCTACCCAGT
GGTGGACTCATTATTATGGATAGAACGCCTGCTGGCAGCCGGAGTCACGACGCTACAACTGCGCATTAAAAATGCAGACG
ATGCTCAGGTGGAGCAAGATATTGTCGCGGCCATTGAATTAGGAAAACGCTATCAGGCGCGCTTATTTATCAATGATTAC
TGGCAGTTGGCGGTTAAACACGGCGCTTATGGCGTTCATCTCGGTCAGGAAGATCTGGAAGCCGCTGATTTAGCGGCAAT
TCAGCAAGCGGGGTTGCGGCTCGGCATTTCTACCCATGATGAACATGAGTTGGCGGTGGCAAAAACACTGCGCCCCTCTT
ATATCGCACTGGGGCATATTTTTCCCACTCAGACCAAACAGATGCCGTCATCCCCACAGGGGTTGGCGTCGTTAAGCCGT
CAGGTGAAGAATACGCCGGATTACCCAACTGTGGCTATCGGTGGCATCAGTATTGAGCGGGTGCCACACGTATTAGCAAC
CGGCGTCGGCAGTGTCGCCGTGGTCAGCGCCATTACGTTGGCAAGTGACTGGCAACGGGCAACCGCACAATTACTGCACC
TGATCGAGGGTAAGGAGTTGGCTGATGAAAAATGA

Upstream 100 bases:

>100_bases
AATATGCTGCAATCAGGGATGGAAAAAATGTCGGCCGAGTTTCGCTCCCGTGGCAGTGAGCTATACCACCGCCCGGCGAA
TCTGAGTGCGGAGGCCAATA

Downstream 100 bases:

>100_bases
CCAGACGCTAAGTGACAGTGAATTTTTACGCTATAGCCGCCAACTGCTACTGGAAGATATCGGCCCGGAAGGGCAACGAA
AGCTAAAGGGTGCCAGTGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDY
WQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSR
QVKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Sequences:

>Translated_224_residues
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDY
WQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSR
QVKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
>Mature_223_residues
SQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYW
QLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQ
VKNTPDYPTVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=202, Percent_Identity=71.2871287128713, Blast_Score=294, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6325042, Length=228, Percent_Identity=28.0701754385965, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24186; Mature: 24055

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQD
CCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IVAAIELGKRYQARLFINDYWQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHD
HHHHHHHCCHHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
EHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIER
CHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHH
VPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SQLDALSPVATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQD
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IVAAIELGKRYQARLFINDYWQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHD
HHHHHHHCCHHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCC
EHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIER
CHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHH
VPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA