Click here to switch to the map view.

The map label for this gene is gloA [C]

Identifier: 108801894

GI number: 108801894

Start: 5221837

End: 5222274

Strand: Reverse

Name: gloA [C]

Synonym: Mmcs_4931

Alternate gene names: 108801894

Gene position: 5222274-5221837 (Counterclockwise)

Preceding gene: 108801895

Following gene: 108801892

Centisome position: 91.53

GC content: 67.81

Gene sequence:

>438_bases
GTGGACATCAGCCATGTGGGCCTGCGGGTACGCGATCTCGAGATGGCCACGAAGTTCTACACGGCACTCGGCTTCACCGA
GGTGAAGCGCCTGACGGTCCCCGACCAGATGGCGCAGGGCCTGCTCGGACTGGCGCCGCCCATCGGATTCGAGGCGGTGT
ATCTGCGCAACGGCGGTGTCGTGCTGCAATTGCTGACCTTCTCGGGCCACCCGGCGCCGGAGGAACCCGAACGCGGAATG
GTCGGAGCCGGTTTGACGCATCTGTCCATCGCGGTTGCGGATCTCGCCGCGGCCTGTGACGCGGTGACGGCCGCCGGGGG
AGCGGTGGTCGCCGACCCGGGCGGCGGATTCGCCTGTATGGTCCGCGATCCCGACGGTCAACTCCTCGAACTGGTCAACG
AACGCGTCCGTCCGGTGCCGGTCACCGAAGCTCGCTGA

Upstream 100 bases:

>100_bases
TCGGCTACGGCGTGCTGTTCTTCGCCTCCGACGCCTCGAGTTACTGCTCCGGGCAGACGCTCTACATGCACGGCGGCCCG
GGCCCGGCGGGGGTGTGAGC

Downstream 100 bases:

>100_bases
CCAACGCCGCATATCGGTCGAGGAACGGCAATGTCGTCGAGGGGTCGTCGCCGTCGCGTCGGCCGCCACCCACGACCACC
CGGGTGAACCCCAGGTCGCG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: (R)-S-Lactoylglutathione [C]

Alternate protein names: Glyoxalase Family Protein

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MDISHVGLRVRDLEMATKFYTALGFTEVKRLTVPDQMAQGLLGLAPPIGFEAVYLRNGGVVLQLLTFSGHPAPEEPERGM
VGAGLTHLSIAVADLAAACDAVTAAGGAVVADPGGGFACMVRDPDGQLLELVNERVRPVPVTEAR

Sequences:

>Translated_145_residues
MDISHVGLRVRDLEMATKFYTALGFTEVKRLTVPDQMAQGLLGLAPPIGFEAVYLRNGGVVLQLLTFSGHPAPEEPERGM
VGAGLTHLSIAVADLAAACDAVTAAGGAVVADPGGGFACMVRDPDGQLLELVNERVRPVPVTEAR
>Mature_145_residues
MDISHVGLRVRDLEMATKFYTALGFTEVKRLTVPDQMAQGLLGLAPPIGFEAVYLRNGGVVLQLLTFSGHPAPEEPERGM
VGAGLTHLSIAVADLAAACDAVTAAGGAVVADPGGGFACMVRDPDGQLLELVNERVRPVPVTEAR

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.4.1.5 [C]

Molecular weight: Translated: 15150; Mature: 15150

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISHVGLRVRDLEMATKFYTALGFTEVKRLTVPDQMAQGLLGLAPPIGFEAVYLRNGGV
CCCCCCCEEEEHHHHHHHHHHHHCHHHHHEECCCHHHHHHHHHCCCCCCCEEEEEECCCE
VLQLLTFSGHPAPEEPERGMVGAGLTHLSIAVADLAAACDAVTAAGGAVVADPGGGFACM
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
VRDPDGQLLELVNERVRPVPVTEAR
EECCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDISHVGLRVRDLEMATKFYTALGFTEVKRLTVPDQMAQGLLGLAPPIGFEAVYLRNGGV
CCCCCCCEEEEHHHHHHHHHHHHCHHHHHEECCCHHHHHHHHHCCCCCCCEEEEEECCCE
VLQLLTFSGHPAPEEPERGMVGAGLTHLSIAVADLAAACDAVTAAGGAVVADPGGGFACM
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
VRDPDGQLLELVNERVRPVPVTEAR
EECCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: Reduced glutathione; Methylglyoxal [C]

Specific reaction: Reduced glutathione + Methylglyoxal --> (R)-S-Lactoylglutathione [C]

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA