Click here to switch to the map view.

The map label for this gene is ask [H]

Identifier: 108801852

GI number: 108801852

Start: 5177718

End: 5178983

Strand: Reverse

Name: ask [H]

Synonym: Mmcs_4889

Alternate gene names: 108801852

Gene position: 5178983-5177718 (Counterclockwise)

Preceding gene: 108801863

Following gene: 108801851

Centisome position: 90.77

GC content: 67.14

Gene sequence:

>1266_bases
GTGGCGCTCGTCGTCCAGAAATACGGTGGGTCCTCGGTGTCGGACGCCGAGCGTATCCGCCGCGTCGCCGAACGCATCGT
CGAGACCAAGAAGGCGGGCAACGACGTGGTCGTCGTCGTCTCGGCGATGGGTGACACCACCGACGACCTGCTCGACCTGG
CCAAGCAGGTGTGCCCGGCCCCGCCGGCGCGTGAACTCGACATGCTGCTCACCGCCGGTGAGCGCATCTCCAACGCCCTG
GTGGCGATGGCCATCGAGTCCCTCGGCGCCCAGGCCCGCTCGTTCACCGGCTCGCAGGCGGGCGTCGTCACCACCGGCAC
CCACGGCAACGCCAAGATCATCGACGTCACCCCGACCCGGCTGCGGTCGGCGCTCGACGAGGGCCAGATCGTGCTCGTCG
CCGGATTCCAGGGTGTGAGTCAGGACACCAAGGACGTCACCACGCTCGGCCGCGGCGGGTCGGACACCACCGCGGTCGCC
GTGGCGGCGGCGCTCAACGCCGACGTCTGTGAGATCTACACCGACGTCGACGGCGTCTACACCGCCGACCCGCGCATCGT
GCCCAACGCGCGCAAACTCGACACCGTCAGTTTCGAGGAGATGCTCGAGATGGCGGCGTGCGGCGCCAAGGTGCTCATGC
TGCGCTGTGTGGAGTACGCCCGCCGCTATGACCTGCCCATCCACGTGCGCTCGTCGTACTCGGACAAACCCGGCACCATC
GTCAAAGGATCGATCGAGGACATCGCCATGGAAGACGCCATCCTGACCGGAGTTGCTCACGACCGCAGTGAGTCCAAGGT
CACCGTCGTGGGCCTGCCCGACGTCCCCGGTTACGCCGCCAAGGTGTTCCGCGCGGTCGCCGACGCCGACGTGAACATCG
ACATGGTGCTGCAGAACATCAGCAAGATCGAGGACGGGAAGACCGACATCACGTTCACGTGTGCGCGGGACAACGCCCCC
GGTGCGGTGGAGAAGCTGACCTCGCTGCAGGACGAGATCGGGTTCACCCGCGTGCTCTACGACGACCACATCGGCAAGGT
GTCGTTGATCGGCGCCGGGATGCGCAGCCATCCGGGCGTCACCGCGACGTTCTGCGAGGCGCTCGCGGAGGTCGGGGTGA
ACATCGACCTGATCTCGACATCGGAGATCCGGATCTCCGTGCTGGTCAAGGACACGGAATTGGACAAGGCGGTCGCCGCG
TTGCACGAGGCGTTCGGCCTCGGCGGCGACGAAGAGGCCGTGGTGTACGCAGGGACGGGACGGTAA

Upstream 100 bases:

>100_bases
CCGGTGTGGCGTGCGTCGCCCCCGGACGGCGGAGGGAAAAAGCAGGTGCGCGCAGCCCTTATCCTTGGTGGGACTTTTTC
CCGCGCGAAAGGCTAGTTCA

Downstream 100 bases:

>100_bases
TTCCGGATGGTCAACATCGGAGTGGTCGGCGCGACCGGCCAGGTCGGCCAGGTCATGCGCACCCTGCTCGAGGAGCGCGA
TTTCCCGGCCACCAGCGTCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MALVVQKYGGSSVSDAERIRRVAERIVETKKAGNDVVVVVSAMGDTTDDLLDLAKQVCPAPPARELDMLLTAGERISNAL
VAMAIESLGAQARSFTGSQAGVVTTGTHGNAKIIDVTPTRLRSALDEGQIVLVAGFQGVSQDTKDVTTLGRGGSDTTAVA
VAAALNADVCEIYTDVDGVYTADPRIVPNARKLDTVSFEEMLEMAACGAKVLMLRCVEYARRYDLPIHVRSSYSDKPGTI
VKGSIEDIAMEDAILTGVAHDRSESKVTVVGLPDVPGYAAKVFRAVADADVNIDMVLQNISKIEDGKTDITFTCARDNAP
GAVEKLTSLQDEIGFTRVLYDDHIGKVSLIGAGMRSHPGVTATFCEALAEVGVNIDLISTSEIRISVLVKDTELDKAVAA
LHEAFGLGGDEEAVVYAGTGR

Sequences:

>Translated_421_residues
MALVVQKYGGSSVSDAERIRRVAERIVETKKAGNDVVVVVSAMGDTTDDLLDLAKQVCPAPPARELDMLLTAGERISNAL
VAMAIESLGAQARSFTGSQAGVVTTGTHGNAKIIDVTPTRLRSALDEGQIVLVAGFQGVSQDTKDVTTLGRGGSDTTAVA
VAAALNADVCEIYTDVDGVYTADPRIVPNARKLDTVSFEEMLEMAACGAKVLMLRCVEYARRYDLPIHVRSSYSDKPGTI
VKGSIEDIAMEDAILTGVAHDRSESKVTVVGLPDVPGYAAKVFRAVADADVNIDMVLQNISKIEDGKTDITFTCARDNAP
GAVEKLTSLQDEIGFTRVLYDDHIGKVSLIGAGMRSHPGVTATFCEALAEVGVNIDLISTSEIRISVLVKDTELDKAVAA
LHEAFGLGGDEEAVVYAGTGR
>Mature_420_residues
ALVVQKYGGSSVSDAERIRRVAERIVETKKAGNDVVVVVSAMGDTTDDLLDLAKQVCPAPPARELDMLLTAGERISNALV
AMAIESLGAQARSFTGSQAGVVTTGTHGNAKIIDVTPTRLRSALDEGQIVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAV
AAALNADVCEIYTDVDGVYTADPRIVPNARKLDTVSFEEMLEMAACGAKVLMLRCVEYARRYDLPIHVRSSYSDKPGTIV
KGSIEDIAMEDAILTGVAHDRSESKVTVVGLPDVPGYAAKVFRAVADADVNIDMVLQNISKIEDGKTDITFTCARDNAPG
AVEKLTSLQDEIGFTRVLYDDHIGKVSLIGAGMRSHPGVTATFCEALAEVGVNIDLISTSEIRISVLVKDTELDKAVAAL
HEAFGLGGDEEAVVYAGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=27.2138228941685, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=25.9825327510917, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790376, Length=460, Percent_Identity=24.3478260869565, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=401, Percent_Identity=24.9376558603491, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44431; Mature: 44300

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGSSVSDAERIRRVAERIVETKKAGNDVVVVVSAMGDTTDDLLDLAKQVCPA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPARELDMLLTAGERISNALVAMAIESLGAQARSFTGSQAGVVTTGTHGNAKIIDVTPTR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEECCCCCEEEEECCHHH
LRSALDEGQIVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAVAAALNADVCEIYTDVDGVY
HHHHHCCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHEECCCCEE
TADPRIVPNARKLDTVSFEEMLEMAACGAKVLMLRCVEYARRYDLPIHVRSSYSDKPGTI
ECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
VKGSIEDIAMEDAILTGVAHDRSESKVTVVGLPDVPGYAAKVFRAVADADVNIDMVLQNI
EECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SKIEDGKTDITFTCARDNAPGAVEKLTSLQDEIGFTRVLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCC
TATFCEALAEVGVNIDLISTSEIRISVLVKDTELDKAVAALHEAFGLGGDEEAVVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C
>Mature Secondary Structure 
ALVVQKYGGSSVSDAERIRRVAERIVETKKAGNDVVVVVSAMGDTTDDLLDLAKQVCPA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPARELDMLLTAGERISNALVAMAIESLGAQARSFTGSQAGVVTTGTHGNAKIIDVTPTR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEECCCCCEEEEECCHHH
LRSALDEGQIVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAVAAALNADVCEIYTDVDGVY
HHHHHCCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCHHHHEECCCCEE
TADPRIVPNARKLDTVSFEEMLEMAACGAKVLMLRCVEYARRYDLPIHVRSSYSDKPGTI
ECCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
VKGSIEDIAMEDAILTGVAHDRSESKVTVVGLPDVPGYAAKVFRAVADADVNIDMVLQNI
EECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SKIEDGKTDITFTCARDNAPGAVEKLTSLQDEIGFTRVLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCC
TATFCEALAEVGVNIDLISTSEIRISVLVKDTELDKAVAALHEAFGLGGDEEAVVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7910936 [H]