Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 108801739

GI number: 108801739

Start: 5052494

End: 5053456

Strand: Reverse

Name: tilS [H]

Synonym: Mmcs_4776

Alternate gene names: 108801739

Gene position: 5053456-5052494 (Counterclockwise)

Preceding gene: 108801740

Following gene: 108801738

Centisome position: 88.57

GC content: 75.49

Gene sequence:

>963_bases
ATGGATCGACCGCGTGCTGTAGCCGCGCTGCGCACCGAGGTGGCCCGGTTCGCCCGCCGCCACCTGGCCGGCACGCAACG
CTGGTGTGTGGCGCTGTCCGGCGGAGCCGATTCGCTGGCGCTGACCGCGGTGGCCGCCGACATCCTGCCCACCACCGCCC
TGATCGTCGACCATGGGCTGCAACCGGATTCGGCTGCCGTCGCCGAGACCGCCCGTGCCGAAGCGCTGCGTCTCGGCTGC
GCCGACGCGCACGTGCTGCGGGTCGACGTCGGCACCTCGGGCGGACCCGAGGCCGCCGCCCGCACGGCCCGCTACGGCGC
GTTGCGCGCGGCCCACGGAGGCGCTCCGGTCCTGCTCGGCCACACCCTCGACGACCAGGCGGAGACGGTCCTGCTCGGGC
TGGGGCGCGGCTCGGGTCCGCGGTCGATCGCCGGAATGCGGGTGTGCGATCCGCCCTGGTACCGCCCCCTGCTGGGGGTG
CGCCGCGCGGCGACCGCCGCCGCATGCACCGAACTCGGGCTACATCCCTGGGTGGATCCGCACAACAGCGATCCGCGCTA
CACCCGCAGCCGGCTGCGCACCGAGGTGCTGCCGCTGCTCGAAGACGTCCTCGCCGGGGGAGTGGCCGCAGCGCTGGCGC
GCACCGCCGCCGCGCTGCAGGACGACAACGACACCCTCGACGCGCAGGCCGATGCGGCGTTGGCCGAGGTCACCGCCGGA
GCGGGACTCGACACCGCACGGCTGGCGGAGTTGCCCGCCGCCATCCGGCGGCGGGTGATCCGCAGCTGGCTGCTCGCCGG
TGGCGCCCGCGGGCTGACCGACACGCAGATCCAGCGGGTCGACGCCCTGGTGAGCGCCTGGCACGGACAGGGCGGCGTGG
CGGTCCCGTCCGCGTCGGTGCGTCAGCGGCTGATCGCGGGACGCCGCGACGGGCTGCTGACCCTGCATCACGAGCCGGTG
TGA

Upstream 100 bases:

>100_bases
TCGACCACGTCGTGGCCAAGGTCGGGATGGAGCGGTTCAACACCGTCTGGTCCGGACCCGAAACCCTGCCGCTGCCAACA
GAAGTCGACGAGCCGCAGCG

Downstream 100 bases:

>100_bases
GAGCGGCCGCGTTGGTTCCGGTCCCGACGGCATGGCACGCTGTGGGCGTGCCTGCGCATACACCCGACCTGTACGAGGGC
GACATCAAGTCGGTGCTGCT

Product: tRNA(Ile)-lysidine synthetase-like protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDRPRAVAALRTEVARFARRHLAGTQRWCVALSGGADSLALTAVAADILPTTALIVDHGLQPDSAAVAETARAEALRLGC
ADAHVLRVDVGTSGGPEAAARTARYGALRAAHGGAPVLLGHTLDDQAETVLLGLGRGSGPRSIAGMRVCDPPWYRPLLGV
RRAATAAACTELGLHPWVDPHNSDPRYTRSRLRTEVLPLLEDVLAGGVAAALARTAAALQDDNDTLDAQADAALAEVTAG
AGLDTARLAELPAAIRRRVIRSWLLAGGARGLTDTQIQRVDALVSAWHGQGGVAVPSASVRQRLIAGRRDGLLTLHHEPV

Sequences:

>Translated_320_residues
MDRPRAVAALRTEVARFARRHLAGTQRWCVALSGGADSLALTAVAADILPTTALIVDHGLQPDSAAVAETARAEALRLGC
ADAHVLRVDVGTSGGPEAAARTARYGALRAAHGGAPVLLGHTLDDQAETVLLGLGRGSGPRSIAGMRVCDPPWYRPLLGV
RRAATAAACTELGLHPWVDPHNSDPRYTRSRLRTEVLPLLEDVLAGGVAAALARTAAALQDDNDTLDAQADAALAEVTAG
AGLDTARLAELPAAIRRRVIRSWLLAGGARGLTDTQIQRVDALVSAWHGQGGVAVPSASVRQRLIAGRRDGLLTLHHEPV
>Mature_320_residues
MDRPRAVAALRTEVARFARRHLAGTQRWCVALSGGADSLALTAVAADILPTTALIVDHGLQPDSAAVAETARAEALRLGC
ADAHVLRVDVGTSGGPEAAARTARYGALRAAHGGAPVLLGHTLDDQAETVLLGLGRGSGPRSIAGMRVCDPPWYRPLLGV
RRAATAAACTELGLHPWVDPHNSDPRYTRSRLRTEVLPLLEDVLAGGVAAALARTAAALQDDNDTLDAQADAALAEVTAG
AGLDTARLAELPAAIRRRVIRSWLLAGGARGLTDTQIQRVDALVSAWHGQGGVAVPSASVRQRLIAGRRDGLLTLHHEPV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=290, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 33353; Mature: 33353

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPRAVAALRTEVARFARRHLAGTQRWCVALSGGADSLALTAVAADILPTTALIVDHGL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
QPDSAAVAETARAEALRLGCADAHVLRVDVGTSGGPEAAARTARYGALRAAHGGAPVLLG
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
HTLDDQAETVLLGLGRGSGPRSIAGMRVCDPPWYRPLLGVRRAATAAACTELGLHPWVDP
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HNSDPRYTRSRLRTEVLPLLEDVLAGGVAAALARTAAALQDDNDTLDAQADAALAEVTAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
AGLDTARLAELPAAIRRRVIRSWLLAGGARGLTDTQIQRVDALVSAWHGQGGVAVPSASV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHH
RQRLIAGRRDGLLTLHHEPV
HHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MDRPRAVAALRTEVARFARRHLAGTQRWCVALSGGADSLALTAVAADILPTTALIVDHGL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
QPDSAAVAETARAEALRLGCADAHVLRVDVGTSGGPEAAARTARYGALRAAHGGAPVLLG
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
HTLDDQAETVLLGLGRGSGPRSIAGMRVCDPPWYRPLLGVRRAATAAACTELGLHPWVDP
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HNSDPRYTRSRLRTEVLPLLEDVLAGGVAAALARTAAALQDDNDTLDAQADAALAEVTAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
AGLDTARLAELPAAIRRRVIRSWLLAGGARGLTDTQIQRVDALVSAWHGQGGVAVPSASV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHH
RQRLIAGRRDGLLTLHHEPV
HHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]