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The map label for this gene is yadF [C]

Identifier: 108801708

GI number: 108801708

Start: 5022736

End: 5023338

Strand: Reverse

Name: yadF [C]

Synonym: Mmcs_4745

Alternate gene names: 108801708

Gene position: 5023338-5022736 (Counterclockwise)

Preceding gene: 108801711

Following gene: 108801707

Centisome position: 88.04

GC content: 70.15

Gene sequence:

>603_bases
GTGACCGCGTGGAAGGCACTCAAAGAGGGTAACGAGCGCTTCGTCGCTGGTCGGCCGGAGCATCCGAGCCAGAGCATCGA
CTACCGCGCCAGCCTCGCCGAGGGGCAGCGCCCCACCACGGTGGTGTTCGGCTGCGGCGACAGCCGGGTGGCCGCCGAGA
TCATCTTCGACCAGGGCCTCGGCGACATGTTCGTGGTGCGCACCGCCGGCCACGTCATCGACTCCGCGGTGCTCGGCTCA
ATCGAGTTCGCGGTGACGGTGCTCGAGGTGCCGCTGATCGTGGTGCTCGGGCACGACAGCTGCGGTGCGGTGAAGGCCAC
GCTCGCGGCGCTCGACGAGGGTGTGGTGCCCGGCGGGTACGTGCGCGACATCGTCGAGCGGGTGACGCCGTCGATCCTGC
TGGGCAGGCGGGACGGGTTGAGCCGGGTCGACGAGTTCGAGGAGCGGCACGTCAACGAGACCGCGGTGCAGTTGCGCGAC
CGGTCCAGTGCGATCGCCTCCCGCATCGAGGCGGGCAAGGTCGCGATCGCCGGTGTCACCTACCACCTGGCCGACGGCCG
GGCCCAGTTGCGCGCTCACCTGGGTGACATCGGCGAAGCCTAG

Upstream 100 bases:

>100_bases
AGATCCCGCTGCTCGCGGTCGTACCACGCCAGAACGTCGGCCGGATCGATCATCTCTCAACCACTCAGGCACAATATGCG
GCATGCCGAACTCCAGCCCG

Downstream 100 bases:

>100_bases
CGCGACACGCCGAGGCACCTCGGCCGATCGGGCCTGACCGGGCTTTACCGTGGTCGTGTGCTGGATCTGGAACCGCAAGG
CCCACTGCCTACGCAGATTT

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTAWKALKEGNERFVAGRPEHPSQSIDYRASLAEGQRPTTVVFGCGDSRVAAEIIFDQGLGDMFVVRTAGHVIDSAVLGS
IEFAVTVLEVPLIVVLGHDSCGAVKATLAALDEGVVPGGYVRDIVERVTPSILLGRRDGLSRVDEFEERHVNETAVQLRD
RSSAIASRIEAGKVAIAGVTYHLADGRAQLRAHLGDIGEA

Sequences:

>Translated_200_residues
MTAWKALKEGNERFVAGRPEHPSQSIDYRASLAEGQRPTTVVFGCGDSRVAAEIIFDQGLGDMFVVRTAGHVIDSAVLGS
IEFAVTVLEVPLIVVLGHDSCGAVKATLAALDEGVVPGGYVRDIVERVTPSILLGRRDGLSRVDEFEERHVNETAVQLRD
RSSAIASRIEAGKVAIAGVTYHLADGRAQLRAHLGDIGEA
>Mature_199_residues
TAWKALKEGNERFVAGRPEHPSQSIDYRASLAEGQRPTTVVFGCGDSRVAAEIIFDQGLGDMFVVRTAGHVIDSAVLGSI
EFAVTVLEVPLIVVLGHDSCGAVKATLAALDEGVVPGGYVRDIVERVTPSILLGRRDGLSRVDEFEERHVNETAVQLRDR
SSAIASRIEAGKVAIAGVTYHLADGRAQLRAHLGDIGEA

Specific function: Catalyzes the reversible hydration of carbon dioxide to form bicarbonate [H]

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=164, Percent_Identity=31.7073170731707, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786534, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 21327; Mature: 21196

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAWKALKEGNERFVAGRPEHPSQSIDYRASLAEGQRPTTVVFGCGDSRVAAEIIFDQGL
CCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCC
GDMFVVRTAGHVIDSAVLGSIEFAVTVLEVPLIVVLGHDSCGAVKATLAALDEGVVPGGY
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCHH
VRDIVERVTPSILLGRRDGLSRVDEFEERHVNETAVQLRDRSSAIASRIEAGKVAIAGVT
HHHHHHHHCHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
YHLADGRAQLRAHLGDIGEA
EEECCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAWKALKEGNERFVAGRPEHPSQSIDYRASLAEGQRPTTVVFGCGDSRVAAEIIFDQGL
CHHHHHHCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCC
GDMFVVRTAGHVIDSAVLGSIEFAVTVLEVPLIVVLGHDSCGAVKATLAALDEGVVPGGY
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCHH
VRDIVERVTPSILLGRRDGLSRVDEFEERHVNETAVQLRDRSSAIASRIEAGKVAIAGVT
HHHHHHHHCHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
YHLADGRAQLRAHLGDIGEA
EEECCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA