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The map label for this gene is lcfB [H]

Identifier: 108801612

GI number: 108801612

Start: 4924753

End: 4926258

Strand: Reverse

Name: lcfB [H]

Synonym: Mmcs_4649

Alternate gene names: 108801612

Gene position: 4926258-4924753 (Counterclockwise)

Preceding gene: 108801613

Following gene: 108801610

Centisome position: 86.34

GC content: 67.13

Gene sequence:

>1506_bases
ATGTATCCGGGTACCCATGCCCAGATCGCGCCGGACCGCCCCGCGGTGATCGTCGCCGAGACCGGTGAGCAGGTGAGCTA
TCGGCAGCTCGACGACGATTCCGCGGCGCTGGCCCGGGTGCTCTACGACGCGGGTCTGCGGACCGGCGACGTCGTGGCGC
TGTTGTCCGACAACTCCCCGGAAGCCCTGGTGGTGTTGTGGGCGGCGCTGCGCTCGGGGCTGTACATCACCGCGATCAAC
CACCACCTCACTGCACCCGAAGCCGACTACATCGTCGGTGACTCGGGCGCGCGGGTGCTCGTCGCGTCCGCAGCCCTGGA
CGGGTTGGCAGCCAAGGTGGGTGCGGACCTGCCGCTGCGGCTGTCCTTCGGCGGCGAGATCGACGGATTCGGCTCGTTCG
AGGCCGCGCTGGCCGGGGCGGGTCCGCGGCTGACCGAGCAACCGTGCGGGGCGGTCATGCTGTATTCGTCGGGAACCACG
GGATTCCCGAAGGGTATCCAGCCCGACCTACCGGGCCGCGACGTCGACGCGCCGGGCGATCCGATCGTCGCGATCGCCCG
CGCGTTCTACGACATCAGCGAGTCCGACATCTACTACTCGTCCGCGCCGATCTATCACGCCGCACCGCTGCGGTGGTGTT
CGATGGTGCACGCACTCGGCGGAACCGTGGTGCTGGCCAAGCGATTCGATGCCCAGGCCACACTCGGGCACGTCGAGCGG
TACCGGATCACCGTCACCCAGATGGTGCCGACGATGTTCGTGCGGCTACTCAAACTCGACGCCGACGTCCGCACCCGGTA
CGACGTGCCGAGCCTGCGGGCGGTCATCCATGCCGCCGCGCCGTGCCCGGTCGATGTCAAACACGCGATGATCGACTGGC
TCGGCCCGATCGTCTACGAGTACTACAGTTCGACCGAGGCGCATGGGATGACGTTCATCGACAGCCCGGACTGGCTGGCC
CATCCCGGATCGGTCGGTCGCAGCGTTCTGGGCGATCTGCACATCTGCGACGACGACGGCAACGAGTTGCCGGCGGGACG
GATCGGCACCGTCTACTTCGAGCGGGACCGCCTACCGTTCCGGTATCTCAACGACCCGGAGAAGACGGCGGCGGCCCAGC
ATCCCGCACATCCGTTCTGGACCACGGTCGGCGATCTCGGCTCGGTCGACGAGGACGGCTATCTGTACCTGGCCGACCGA
AAGTCGTTCATGATCATCTCCGGCGGGGTGAACATCTACCCGCAGGAAACCGAGAACGCGCTGACCATGCACCCCGCCGT
GCACGACGTGGCCGTCATCGGTGTCCCCGATCCCGAGATGGGGGAACAGGTCAAGGCGGTCATCCAACTCGTCGAAGGTA
TTCGCGGCAGCGACGAACTGGCGCGCGAGCTCATCGACTACACGCGGTCGCGGATCGCGCACTACAAGGCGCCCCGCTCG
GTGGAGTTCGTCGACGAACTGCCACGCACACCGACCGGGAAGCTGGTGAAGGGTCTGCTGCGGTGA

Upstream 100 bases:

>100_bases
AGCCCAAGTTCGTCCACATCGACCTGACCAACGACCGTCGCAAACGTGCCTTCGCCGTGGTCGTATCCGCGGCGGCAGCC
GAATCCGACTGAGGGACAAC

Downstream 100 bases:

>100_bases
ACGCGGGTCACACCCCGGCCGGGTGAACCACGCCGCCGAGTTGGGCGGTCACCGCGTCGGCGGTCGCCACCACGGCGCGG
GCCCGTTCACTGATCTCGTT

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MYPGTHAQIAPDRPAVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAIN
HHLTAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSSGTT
GFPKGIQPDLPGRDVDAPGDPIVAIARAFYDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFDAQATLGHVER
YRITVTQMVPTMFVRLLKLDADVRTRYDVPSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMTFIDSPDWLA
HPGSVGRSVLGDLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADR
KSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRS
VEFVDELPRTPTGKLVKGLLR

Sequences:

>Translated_501_residues
MYPGTHAQIAPDRPAVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAIN
HHLTAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSSGTT
GFPKGIQPDLPGRDVDAPGDPIVAIARAFYDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFDAQATLGHVER
YRITVTQMVPTMFVRLLKLDADVRTRYDVPSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMTFIDSPDWLA
HPGSVGRSVLGDLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADR
KSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRS
VEFVDELPRTPTGKLVKGLLR
>Mature_501_residues
MYPGTHAQIAPDRPAVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAIN
HHLTAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLRLSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSSGTT
GFPKGIQPDLPGRDVDAPGDPIVAIARAFYDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFDAQATLGHVER
YRITVTQMVPTMFVRLLKLDADVRTRYDVPSLRAVIHAAAPCPVDVKHAMIDWLGPIVYEYYSSTEAHGMTFIDSPDWLA
HPGSVGRSVLGDLHICDDDGNELPAGRIGTVYFERDRLPFRYLNDPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADR
KSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRS
VEFVDELPRTPTGKLVKGLLR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=524, Percent_Identity=32.0610687022901, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI122937307, Length=520, Percent_Identity=31.9230769230769, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=29.3893129770992, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI58082049, Length=502, Percent_Identity=30.2788844621514, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI157311624, Length=508, Percent_Identity=29.1338582677165, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI157311622, Length=508, Percent_Identity=29.1338582677165, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI115511026, Length=510, Percent_Identity=28.8235294117647, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI156151445, Length=536, Percent_Identity=25.7462686567164, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI187761345, Length=526, Percent_Identity=28.5171102661597, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=526, Percent_Identity=28.5171102661597, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI28416953, Length=553, Percent_Identity=24.7739602169982, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI13375727, Length=551, Percent_Identity=24.1379310344828, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI40807357, Length=558, Percent_Identity=24.7311827956989, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI62865631, Length=544, Percent_Identity=23.7132352941176, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI13325055, Length=544, Percent_Identity=23.7132352941176, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI8923896, Length=563, Percent_Identity=22.0248667850799, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI253970489, Length=532, Percent_Identity=22.1804511278195, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI195947378, Length=416, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=27.5925925925926, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI221142682, Length=526, Percent_Identity=27.9467680608365, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=26.8939393939394, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=25.7309941520468, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790505, Length=553, Percent_Identity=23.6889692585895, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786529, Length=548, Percent_Identity=24.4525547445255, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=26.937984496124, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531443, Length=525, Percent_Identity=25.9047619047619, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=506, Percent_Identity=26.4822134387352, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17538037, Length=538, Percent_Identity=26.9516728624535, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=26.3157894736842, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=541, Percent_Identity=26.0628465804067, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=26.0628465804067, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=26.0628465804067, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=26.2569832402235, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=28.0653950953678, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=24.5098039215686, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=497, Percent_Identity=22.5352112676056, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=26.1111111111111, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=526, Percent_Identity=25.4752851711027, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=560, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323182, Length=576, Percent_Identity=23.2638888888889, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21355181, Length=515, Percent_Identity=26.4077669902913, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=26.6536964980545, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581924, Length=536, Percent_Identity=27.4253731343284, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922652, Length=519, Percent_Identity=25.0481695568401, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648255, Length=503, Percent_Identity=26.0437375745527, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648253, Length=503, Percent_Identity=25.844930417495, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI20130357, Length=403, Percent_Identity=27.5434243176179, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356441, Length=509, Percent_Identity=24.75442043222, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24656500, Length=522, Percent_Identity=22.6053639846743, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281365686, Length=364, Percent_Identity=25.2747252747253, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=24.703557312253, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653035, Length=521, Percent_Identity=23.9923224568138, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358303, Length=495, Percent_Identity=22.8282828282828, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552801, Length=553, Percent_Identity=24.4122965641953, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550496, Length=553, Percent_Identity=24.4122965641953, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648260, Length=358, Percent_Identity=25.1396648044693, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=23.8636363636364, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21355225, Length=373, Percent_Identity=26.0053619302949, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648257, Length=510, Percent_Identity=23.7254901960784, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667959, Length=367, Percent_Identity=23.7057220708447, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62472339, Length=367, Percent_Identity=23.7057220708447, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667955, Length=367, Percent_Identity=23.7057220708447, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54185; Mature: 54185

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPGTHAQIAPDRPAVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSP
CCCCCCCEECCCCCEEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
EALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLR
CHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHCCCCEEE
LSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSSGTTGFPKGIQPDLPGRDVDAPGD
EECCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PIVAIARAFYDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFDAQATLGHVER
HHHHHHHHHHCCCCCCEEECCCCCEECHHHHHHHHHHHHCCEEEEEECCCCHHHHCCHHE
YRITVTQMVPTMFVRLLKLDADVRTRYDVPSLRAVIHAAAPCPVDVKHAMIDWLGPIVYE
EEEEHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YYSSTEAHGMTFIDSPDWLAHPGSVGRSVLGDLHICDDDGNELPAGRIGTVYFERDRLPF
HHCCCCCCCEEEECCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCH
RYLNDPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENA
HCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCCCE
LTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRS
EEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VEFVDELPRTPTGKLVKGLLR
HHHHHHCCCCCHHHHHHHHHC
>Mature Secondary Structure
MYPGTHAQIAPDRPAVIVAETGEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSP
CCCCCCCEECCCCCEEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
EALVVLWAALRSGLYITAINHHLTAPEADYIVGDSGARVLVASAALDGLAAKVGADLPLR
CHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHCCCCEEE
LSFGGEIDGFGSFEAALAGAGPRLTEQPCGAVMLYSSGTTGFPKGIQPDLPGRDVDAPGD
EECCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
PIVAIARAFYDISESDIYYSSAPIYHAAPLRWCSMVHALGGTVVLAKRFDAQATLGHVER
HHHHHHHHHHCCCCCCEEECCCCCEECHHHHHHHHHHHHCCEEEEEECCCCHHHHCCHHE
YRITVTQMVPTMFVRLLKLDADVRTRYDVPSLRAVIHAAAPCPVDVKHAMIDWLGPIVYE
EEEEHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
YYSSTEAHGMTFIDSPDWLAHPGSVGRSVLGDLHICDDDGNELPAGRIGTVYFERDRLPF
HHCCCCCCCEEEECCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCH
RYLNDPEKTAAAQHPAHPFWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENA
HCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEECCCEEEEEECCCEECCCCCCCE
LTMHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRS
EEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
VEFVDELPRTPTGKLVKGLLR
HHHHHHCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]