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The map label for this gene is yngI [H]

Identifier: 108801590

GI number: 108801590

Start: 4900213

End: 4901862

Strand: Reverse

Name: yngI [H]

Synonym: Mmcs_4627

Alternate gene names: 108801590

Gene position: 4901862-4900213 (Counterclockwise)

Preceding gene: 108801593

Following gene: 108801589

Centisome position: 85.92

GC content: 66.3

Gene sequence:

>1650_bases
GTGGCCCTGAACATCGCCGATCTTGCCGAGCACGCCATCGACGCCGTGCCAGACCGTGTCGCCCTGATCAGCGGCGACGA
AACGCTGACCTACGGCGAACTCGAGGAGAGAGCCAACCGCCTGGCGCACTACCTGATCGACCGGGGCGTGAAGAAGGACG
ACAAGGTCGGTCTGTACTGCCGCAACCGCATCGAGATCGTGATCGGGATGGTCGGCATCGTCAAGGCCGGCGCCATCGCG
GTCAACGTCAACTTCCGCTACGTCGAGGGGGAGTTGCGGTACCTGTTCGAGAACTCCGACATGGTGGCGCTGATCCACGA
GCGTCAGTACAGCCCCCGCGTCGCCAACGTCCTGCCCGACATGCCGGGCCTGAAGACGGTGCTGGTGGTCGAGGACGGCA
GCAGTGAGGACTTCTCCTCCTACGGCGGTGTCGAGTTCTACGCGGCGCTGGGACAGGGTTCGCCCGAACGCGATTTCGGC
CCACGCAGCGAAGACGACATCTACCTGCTGTACACGGGCGGCACCACCGGCTTCCCGAAGGGCGTGATGTGGCGCCACGA
GGACATCTACCGGGTGTTGTTCGGCGGCACCGACTTCGCCACCGGCGAGCCGATCGCCGACGAGTACGGCCTGGCCAAGC
AGGCTGCCGAGAACGCCCCGATGGTCCGCTACCCGATTCCGCCGATGATCCACGGTGCGACGCAGTCGGCCACCTGGATG
GCGCTGTTCGCCGGGCAGACCGTCGTGCTGGTGCCGGAGTTCGACCCCGAAGAGGTGTGGCGGACCTGTGCGCAGCACAA
GGTCAACCTGCTGTTCTTCACCGGTGACGCGATGGCCCGCCCACTGCTCGACGCGCTGGTCGCCCATCAGGCCAACGGCG
ACGAGTTCGACCTGTCCAACCTGTTCCTGTTGGCCAGCACCGCGGCGCTGTTCTCGCCCGCGCTCAAGGACCAACTGCTC
GAACTCCTGCCGAACCGGATCATCACCGACTCGATCGGTTCGTCGGAGACCGGGTTCGGCGGGTCGAGTGTCGTGGCCAA
GGGACAGACCCACACCGGCGGTCCGCGCGTCACCATCGACAAGAACGTCGTGGTGCTCGACGACGACGGCAACGAGGTCA
AGCCCGGCAGCGGTGTCCGCGGGATGATCGCCAAACGCGGCCACATCCCCGTCGGCTACTACAAGGACGAAGAGAAAACC
CGGCAGACGTTCCGAGTCATCAACGGCGTCCGCTACGCGATCCCCGGCGACTACGCCACCGTCGAGGAGGACGGCAGCGT
CACGATGCTCGGCCGCGGTTCGCAGTCGATCAACAGCGGCGGCGAGAAGATCTACCCCGAAGAGGTCGAGGGCGCCCTCA
AGGGCCACCCCGATGTGTTCGACGCCCTCGTGGTCGGCGTGCCCGACGAACGGTACGGACAGTGCGTCGCGGCCGTGATC
GCACGGCGCGAAGGCGCCACCCTGTCGCTGGCCGAACTCGACAGCTTCATCCGCAACGAGATCGCCGGCTACAAGGTGCC
CCGCAAGATCTGGTGGGCCGACGAGATCCAGCGCACCCCGGCCGGGAAGCCCGACTACCGCTGGGCCAAGGAACAGACCG
AGTCGCGTCCCGCGGACGAGACGCACGCCAATCACGTTGGAGCCAAGTAA

Upstream 100 bases:

>100_bases
GTTGCTTTTCGGTGACTTGCGCGTCGCTCACAGACAGTCCGCCTTCCAGCGTCGATGCCACAGACTTGTCAGGAAATGTA
ACACGTTCTAGTTTGGGTCC

Downstream 100 bases:

>100_bases
CCATGCGTACCGAACTGTGCGACCGCTTCGGCATCGAGTATCCGATCTTCGTGTTCACACCGTCGGAGAAGGTCGCCGCC
GCCGTCACGCGCGCCGGCGG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MALNIADLAEHAIDAVPDRVALISGDETLTYGELEERANRLAHYLIDRGVKKDDKVGLYCRNRIEIVIGMVGIVKAGAIA
VNVNFRYVEGELRYLFENSDMVALIHERQYSPRVANVLPDMPGLKTVLVVEDGSSEDFSSYGGVEFYAALGQGSPERDFG
PRSEDDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEPIADEYGLAKQAAENAPMVRYPIPPMIHGATQSATWM
ALFAGQTVVLVPEFDPEEVWRTCAQHKVNLLFFTGDAMARPLLDALVAHQANGDEFDLSNLFLLASTAALFSPALKDQLL
ELLPNRIITDSIGSSETGFGGSSVVAKGQTHTGGPRVTIDKNVVVLDDDGNEVKPGSGVRGMIAKRGHIPVGYYKDEEKT
RQTFRVINGVRYAIPGDYATVEEDGSVTMLGRGSQSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDERYGQCVAAVI
ARREGATLSLAELDSFIRNEIAGYKVPRKIWWADEIQRTPAGKPDYRWAKEQTESRPADETHANHVGAK

Sequences:

>Translated_549_residues
MALNIADLAEHAIDAVPDRVALISGDETLTYGELEERANRLAHYLIDRGVKKDDKVGLYCRNRIEIVIGMVGIVKAGAIA
VNVNFRYVEGELRYLFENSDMVALIHERQYSPRVANVLPDMPGLKTVLVVEDGSSEDFSSYGGVEFYAALGQGSPERDFG
PRSEDDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEPIADEYGLAKQAAENAPMVRYPIPPMIHGATQSATWM
ALFAGQTVVLVPEFDPEEVWRTCAQHKVNLLFFTGDAMARPLLDALVAHQANGDEFDLSNLFLLASTAALFSPALKDQLL
ELLPNRIITDSIGSSETGFGGSSVVAKGQTHTGGPRVTIDKNVVVLDDDGNEVKPGSGVRGMIAKRGHIPVGYYKDEEKT
RQTFRVINGVRYAIPGDYATVEEDGSVTMLGRGSQSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDERYGQCVAAVI
ARREGATLSLAELDSFIRNEIAGYKVPRKIWWADEIQRTPAGKPDYRWAKEQTESRPADETHANHVGAK
>Mature_548_residues
ALNIADLAEHAIDAVPDRVALISGDETLTYGELEERANRLAHYLIDRGVKKDDKVGLYCRNRIEIVIGMVGIVKAGAIAV
NVNFRYVEGELRYLFENSDMVALIHERQYSPRVANVLPDMPGLKTVLVVEDGSSEDFSSYGGVEFYAALGQGSPERDFGP
RSEDDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEPIADEYGLAKQAAENAPMVRYPIPPMIHGATQSATWMA
LFAGQTVVLVPEFDPEEVWRTCAQHKVNLLFFTGDAMARPLLDALVAHQANGDEFDLSNLFLLASTAALFSPALKDQLLE
LLPNRIITDSIGSSETGFGGSSVVAKGQTHTGGPRVTIDKNVVVLDDDGNEVKPGSGVRGMIAKRGHIPVGYYKDEEKTR
QTFRVINGVRYAIPGDYATVEEDGSVTMLGRGSQSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDERYGQCVAAVIA
RREGATLSLAELDSFIRNEIAGYKVPRKIWWADEIQRTPAGKPDYRWAKEQTESRPADETHANHVGAK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=571, Percent_Identity=23.8178633975482, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761345, Length=554, Percent_Identity=27.2563176895307, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI187761343, Length=554, Percent_Identity=27.2563176895307, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI115511026, Length=515, Percent_Identity=24.8543689320388, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI58082049, Length=514, Percent_Identity=25.0972762645914, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI157311624, Length=522, Percent_Identity=24.5210727969349, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI157311622, Length=522, Percent_Identity=24.5210727969349, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI42544132, Length=518, Percent_Identity=21.6216216216216, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI38505220, Length=167, Percent_Identity=29.940119760479, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI122937307, Length=202, Percent_Identity=29.7029702970297, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=560, Percent_Identity=24.2857142857143, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786810, Length=537, Percent_Identity=25.6983240223464, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=26.0956175298805, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693145, Length=547, Percent_Identity=24.3144424131627, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790505, Length=553, Percent_Identity=24.0506329113924, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786529, Length=590, Percent_Identity=23.0508474576271, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=562, Percent_Identity=26.3345195729537, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=562, Percent_Identity=26.3345195729537, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=562, Percent_Identity=26.3345195729537, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=26.1595547309833, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=22.9445506692161, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=535, Percent_Identity=24.2990654205607, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=520, Percent_Identity=23.8461538461538, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=536, Percent_Identity=24.0671641791045, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=520, Percent_Identity=23.6538461538462, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=534, Percent_Identity=22.6591760299625, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=572, Percent_Identity=21.5034965034965, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=552, Percent_Identity=23.1884057971014, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=566, Percent_Identity=25.6183745583039, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=25.2268602540835, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355181, Length=532, Percent_Identity=23.4962406015038, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=24.1904761904762, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45552801, Length=499, Percent_Identity=22.6452905811623, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550496, Length=499, Percent_Identity=22.6452905811623, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356947, Length=529, Percent_Identity=23.8185255198488, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583465, Length=498, Percent_Identity=21.8875502008032, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583463, Length=498, Percent_Identity=21.8875502008032, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364798, Length=498, Percent_Identity=21.8875502008032, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60012; Mature: 59881

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNIADLAEHAIDAVPDRVALISGDETLTYGELEERANRLAHYLIDRGVKKDDKVGLYC
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
RNRIEIVIGMVGIVKAGAIAVNVNFRYVEGELRYLFENSDMVALIHERQYSPRVANVLPD
CCCEEEEEEHHHHHCCCEEEEEEEEEEEECEEEEEEECCCEEEEEECCCCCCHHHHHCCC
MPGLKTVLVVEDGSSEDFSSYGGVEFYAALGQGSPERDFGPRSEDDIYLLYTGGTTGFPK
CCCCEEEEEEECCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GVMWRHEDIYRVLFGGTDFATGEPIADEYGLAKQAAENAPMVRYPIPPMIHGATQSATWM
CCEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEE
ALFAGQTVVLVPEFDPEEVWRTCAQHKVNLLFFTGDAMARPLLDALVAHQANGDEFDLSN
EEECCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHH
LFLLASTAALFSPALKDQLLELLPNRIITDSIGSSETGFGGSSVVAKGQTHTGGPRVTID
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCCEEEEE
KNVVVLDDDGNEVKPGSGVRGMIAKRGHIPVGYYKDEEKTRQTFRVINGVRYAIPGDYAT
CCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCEECCHHHHHHHHHHHCCEEEECCCCCEE
VEEDGSVTMLGRGSQSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDERYGQCVAAVI
EECCCCEEEEECCCCCCCCCCCEECHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHHHH
ARREGATLSLAELDSFIRNEIAGYKVPRKIWWADEIQRTPAGKPDYRWAKEQTESRPADE
HHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECHHHHHCCCCCCCCCCHHHHHHCCCCCCH
THANHVGAK
HHCCCCCCC
>Mature Secondary Structure 
ALNIADLAEHAIDAVPDRVALISGDETLTYGELEERANRLAHYLIDRGVKKDDKVGLYC
CCCHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
RNRIEIVIGMVGIVKAGAIAVNVNFRYVEGELRYLFENSDMVALIHERQYSPRVANVLPD
CCCEEEEEEHHHHHCCCEEEEEEEEEEEECEEEEEEECCCEEEEEECCCCCCHHHHHCCC
MPGLKTVLVVEDGSSEDFSSYGGVEFYAALGQGSPERDFGPRSEDDIYLLYTGGTTGFPK
CCCCEEEEEEECCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GVMWRHEDIYRVLFGGTDFATGEPIADEYGLAKQAAENAPMVRYPIPPMIHGATQSATWM
CCEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEE
ALFAGQTVVLVPEFDPEEVWRTCAQHKVNLLFFTGDAMARPLLDALVAHQANGDEFDLSN
EEECCCEEEEEECCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHH
LFLLASTAALFSPALKDQLLELLPNRIITDSIGSSETGFGGSSVVAKGQTHTGGPRVTID
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCCEEEEE
KNVVVLDDDGNEVKPGSGVRGMIAKRGHIPVGYYKDEEKTRQTFRVINGVRYAIPGDYAT
CCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCEECCHHHHHHHHHHHCCEEEECCCCCEE
VEEDGSVTMLGRGSQSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDERYGQCVAAVI
EECCCCEEEEECCCCCCCCCCCEECHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHHHH
ARREGATLSLAELDSFIRNEIAGYKVPRKIWWADEIQRTPAGKPDYRWAKEQTESRPADE
HHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECHHHHHCCCCCCCCCCHHHHHHCCCCCCH
THANHVGAK
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]