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The map label for this gene is menB [C]

Identifier: 108801570

GI number: 108801570

Start: 4880065

End: 4880961

Strand: Reverse

Name: menB [C]

Synonym: Mmcs_4607

Alternate gene names: 108801570

Gene position: 4880961-4880065 (Counterclockwise)

Preceding gene: 108801573

Following gene: 108801569

Centisome position: 85.55

GC content: 68.34

Gene sequence:

>897_bases
GTGGCAGACGCCGAGCAGGTGACCTACGAGACCCTCGACGACGGGCGCATCGCGCGGATCTGGCTGAACCGGCCGCATAC
GCAGAACGCCCAGTCCCGGACGCTGCTGGTGCAGCTGGACGAGGCGTTCGGGCGCGCGGAGGCCGACGATCAGGTGCGGG
TGGTGATCCTGGCCGCGCGCGGCAAGAACTTCTCCGCCGGCCACGACCTCGGATCCGAGGAGGCCATCGCCGAACGCGGC
CCCGGTCCCGCGCAGCATCCGACGTTCCGGATCAACGGTGCGACCAGGCCCGCCATCGCGGAGAAGATCTACCTGCAGGA
GTGGCACTACTTCTTCGAGAACACCCGGCGGTGGCGCGATCTGCGCAAGATCACGATCGCCCAGGTGCAGGGAAATGCGA
TCTCGGCGGGGCTGATGCTGATCTGGGCGTGCGACCTCATCGTCGCCTCCGACGACGCGAAGTTCAGCGACGTGGTGGCG
GTCCGGCTGGGTATGCCGGGTGTCGAGTACTACGCCCACCCGTGGGAGTTCGGTCCGCGCAAGGCCAAAGAGCTGCTGCT
GACCGGTGATTCGATCGACGCCGACGAGGCGCATCGCCTGGGTATGGTGTCGAAGATCTTCCCGCGCGCCGAACTCGAGG
ACAAGACGGTCGAATTCGCGCGCCGGATCGCCGCACTGCCGACGATGGCAGCACTGCTGGTCAAGGATTCGGTCAACGCC
GCCGCCGACGCGATGGGATTCACCGAGGCACTGCGGCACGGCTTCCACATCCACGAGCTCGGGCATGCGCACTGGGCGGC
GCACAACGAGAACCGCGTGGCCGTCGGCATGCCGCCGGACGTGCCGGACTGGCGCGAGCTGGGCGCACCGAAGTTGGCCC
GGCGCGACGAGCCGTGA

Upstream 100 bases:

>100_bases
CTCGACGTCGCCGATATCGGCTGGATCAGACTTTGCAGGCATGTTCCAACTCTAGGCGACCGGCGCGCGGGCCTGCGACA
CGCCCGCGTAGGGTGCTGCT

Downstream 100 bases:

>100_bases
CGTATAACTAGAACGCGTTTCAGTTATACGGCCTACCTACCGGAGGATTCCCTTGCTGCTTTCCCAGCTCTCACTGGCGG
ATCGCACCTATCTGGTCACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MADAEQVTYETLDDGRIARIWLNRPHTQNAQSRTLLVQLDEAFGRAEADDQVRVVILAARGKNFSAGHDLGSEEAIAERG
PGPAQHPTFRINGATRPAIAEKIYLQEWHYFFENTRRWRDLRKITIAQVQGNAISAGLMLIWACDLIVASDDAKFSDVVA
VRLGMPGVEYYAHPWEFGPRKAKELLLTGDSIDADEAHRLGMVSKIFPRAELEDKTVEFARRIAALPTMAALLVKDSVNA
AADAMGFTEALRHGFHIHELGHAHWAAHNENRVAVGMPPDVPDWRELGAPKLARRDEP

Sequences:

>Translated_298_residues
MADAEQVTYETLDDGRIARIWLNRPHTQNAQSRTLLVQLDEAFGRAEADDQVRVVILAARGKNFSAGHDLGSEEAIAERG
PGPAQHPTFRINGATRPAIAEKIYLQEWHYFFENTRRWRDLRKITIAQVQGNAISAGLMLIWACDLIVASDDAKFSDVVA
VRLGMPGVEYYAHPWEFGPRKAKELLLTGDSIDADEAHRLGMVSKIFPRAELEDKTVEFARRIAALPTMAALLVKDSVNA
AADAMGFTEALRHGFHIHELGHAHWAAHNENRVAVGMPPDVPDWRELGAPKLARRDEP
>Mature_297_residues
ADAEQVTYETLDDGRIARIWLNRPHTQNAQSRTLLVQLDEAFGRAEADDQVRVVILAARGKNFSAGHDLGSEEAIAERGP
GPAQHPTFRINGATRPAIAEKIYLQEWHYFFENTRRWRDLRKITIAQVQGNAISAGLMLIWACDLIVASDDAKFSDVVAV
RLGMPGVEYYAHPWEFGPRKAKELLLTGDSIDADEAHRLGMVSKIFPRAELEDKTVEFARRIAALPTMAALLVKDSVNAA
ADAMGFTEALRHGFHIHELGHAHWAAHNENRVAVGMPPDVPDWRELGAPKLARRDEP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=239, Percent_Identity=28.4518828451883, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI31542718, Length=217, Percent_Identity=31.3364055299539, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=27.9835390946502, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=27.9835390946502, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788597, Length=276, Percent_Identity=27.8985507246377, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787659, Length=219, Percent_Identity=28.7671232876712, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787660, Length=227, Percent_Identity=27.7533039647577, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=29.0566037735849, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=264, Percent_Identity=28.030303030303, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=27.2373540856031, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=231, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=214, Percent_Identity=26.1682242990654, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=30.1310043668122, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=28.7937743190661, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=28.7937743190661, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=30.241935483871, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 33194; Mature: 33063

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAEQVTYETLDDGRIARIWLNRPHTQNAQSRTLLVQLDEAFGRAEADDQVRVVILAAR
CCCCHHCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEEHHHCCCCCCCCCEEEEEEEEC
GKNFSAGHDLGSEEAIAERGPGPAQHPTFRINGATRPAIAEKIYLQEWHYFFENTRRWRD
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRKITIAQVQGNAISAGLMLIWACDLIVASDDAKFSDVVAVRLGMPGVEYYAHPWEFGPR
HHHEEEEEECCCCHHHHHHHHHHHHEEEECCCCCHHHEEEEEECCCCHHHCCCCCCCCCH
KAKELLLTGDSIDADEAHRLGMVSKIFPRAELEDKTVEFARRIAALPTMAALLVKDSVNA
HHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
AADAMGFTEALRHGFHIHELGHAHWAAHNENRVAVGMPPDVPDWRELGAPKLARRDEP
HHHHHHHHHHHHCCCEEEHHCCHHHCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ADAEQVTYETLDDGRIARIWLNRPHTQNAQSRTLLVQLDEAFGRAEADDQVRVVILAAR
CCCHHCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEEHHHCCCCCCCCCEEEEEEEEC
GKNFSAGHDLGSEEAIAERGPGPAQHPTFRINGATRPAIAEKIYLQEWHYFFENTRRWRD
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRKITIAQVQGNAISAGLMLIWACDLIVASDDAKFSDVVAVRLGMPGVEYYAHPWEFGPR
HHHEEEEEECCCCHHHHHHHHHHHHEEEECCCCCHHHEEEEEECCCCHHHCCCCCCCCCH
KAKELLLTGDSIDADEAHRLGMVSKIFPRAELEDKTVEFARRIAALPTMAALLVKDSVNA
HHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
AADAMGFTEALRHGFHIHELGHAHWAAHNENRVAVGMPPDVPDWRELGAPKLARRDEP
HHHHHHHHHHHHCCCEEEHHCCHHHCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA