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The map label for this gene is garR [C]

Identifier: 108801539

GI number: 108801539

Start: 4852759

End: 4853619

Strand: Reverse

Name: garR [C]

Synonym: Mmcs_4576

Alternate gene names: 108801539

Gene position: 4853619-4852759 (Counterclockwise)

Preceding gene: 108801540

Following gene: 108801538

Centisome position: 85.07

GC content: 69.57

Gene sequence:

>861_bases
ATGAGCGATCTGAAGTACGGCTACATCGGCCTGGGGAACATGGGTGCGCCGATGGCCAAGCGGCTGACCGGCTGGCCGGG
CGGGCTCATCGTGTTCGACGTGCGTCCCGACGCCATGGCGCCGCTGGTCGAGGCCGGCGCCGCGCAGGCCGACAACGTCG
CCGACGTCGCCGACGCCGACATCATCAGCATCACCGTCCTCAACGACGAGCAGGTACGCCACGTGGTCGCCGAGCTCGTG
CCGCGCGCCAAGCCCGGCACCGTCATCGCGATCCACTCGACGATCAGCCCCGACACCGCGGTCGAACTCGCCAAGGAGTT
GCGGTCGCAGGACATCCACGTCGTCGACGCCCCGGTCAGCGGCGGGGCGGGCGCTGCCGAGAAGGGCGAGCTGGCCGTCA
TGGTCGGCGCCGAGCGCTCGGTCTACGAGCGCGTCAAGCCGGCGTTCAAACAGTGGGCGTCCGTTGTGGTCCATGCCGGT
GAGCCCGGCGCGGGGACACGGATGAAGTTGGCGCGCAACATGTTGACGTTCACCGGCTTCGCCGCGGCGTGCGAGGCGAT
GACCCTGGCGGAGAAGGCGGGCCTGGATCTGCAGGCGCTGGGCCGGGTGGTTCGCCACAGCGACGCGCAGTCCGGTGGCC
CGGGCGCGATCATCGTCCGCGAGAACATGCGGCCACTCGACCACGACAACTTCCTCTACGACATGTTCCTGCACACCCGC
GGCCTCGCGGAGAAGGATCTGGGGCTCGCGCTGACGCTCGGTGAGGAACTCGACGTCGACCTGCCGCTGGCGCAGGTGGC
GCTGCAGAATCTGGCGGCCGGCCTGGGTGTGCCGCACGTGGAATCCAGCACGGAGGAGTGA

Upstream 100 bases:

>100_bases
TTGTCGGATCAGGCGAAATGGATCACCGGGCAGATCTTCAACGTCGACGGCGGACAGATCATTCGGTGATGAGCGCTTGC
GCGAAGAGCAGAGGGCACAG

Downstream 100 bases:

>100_bases
GCGGAATGGACGAATTGCGCCGCAAGGGTTTGGCGAAGATGAACGAGGTCTACGGCTGGGAGATGCCGGACATGCCCGGC
GACTACTTCGCACTGACCGC

Product: 6-phosphogluconate dehydrogenase protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSDLKYGYIGLGNMGAPMAKRLTGWPGGLIVFDVRPDAMAPLVEAGAAQADNVADVADADIISITVLNDEQVRHVVAELV
PRAKPGTVIAIHSTISPDTAVELAKELRSQDIHVVDAPVSGGAGAAEKGELAVMVGAERSVYERVKPAFKQWASVVVHAG
EPGAGTRMKLARNMLTFTGFAAACEAMTLAEKAGLDLQALGRVVRHSDAQSGGPGAIIVRENMRPLDHDNFLYDMFLHTR
GLAEKDLGLALTLGEELDVDLPLAQVALQNLAAGLGVPHVESSTEE

Sequences:

>Translated_286_residues
MSDLKYGYIGLGNMGAPMAKRLTGWPGGLIVFDVRPDAMAPLVEAGAAQADNVADVADADIISITVLNDEQVRHVVAELV
PRAKPGTVIAIHSTISPDTAVELAKELRSQDIHVVDAPVSGGAGAAEKGELAVMVGAERSVYERVKPAFKQWASVVVHAG
EPGAGTRMKLARNMLTFTGFAAACEAMTLAEKAGLDLQALGRVVRHSDAQSGGPGAIIVRENMRPLDHDNFLYDMFLHTR
GLAEKDLGLALTLGEELDVDLPLAQVALQNLAAGLGVPHVESSTEE
>Mature_285_residues
SDLKYGYIGLGNMGAPMAKRLTGWPGGLIVFDVRPDAMAPLVEAGAAQADNVADVADADIISITVLNDEQVRHVVAELVP
RAKPGTVIAIHSTISPDTAVELAKELRSQDIHVVDAPVSGGAGAAEKGELAVMVGAERSVYERVKPAFKQWASVVVHAGE
PGAGTRMKLARNMLTFTGFAAACEAMTLAEKAGLDLQALGRVVRHSDAQSGGPGAIIVRENMRPLDHDNFLYDMFLHTRG
LAEKDLGLALTLGEELDVDLPLAQVALQNLAAGLGVPHVESSTEE

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=211, Percent_Identity=37.4407582938389, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI40556376, Length=270, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=34.1549295774648, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789092, Length=280, Percent_Identity=33.9285714285714, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786719, Length=272, Percent_Identity=31.9852941176471, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=31.4285714285714, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=210, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24655230, Length=263, Percent_Identity=30.7984790874525, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922568, Length=263, Percent_Identity=30.7984790874525, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574115, Length=276, Percent_Identity=23.5507246376812, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30086; Mature: 29955

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKYGYIGLGNMGAPMAKRLTGWPGGLIVFDVRPDAMAPLVEAGAAQADNVADVADAD
CCCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHCCCC
IISITVLNDEQVRHVVAELVPRAKPGTVIAIHSTISPDTAVELAKELRSQDIHVVDAPVS
EEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCC
GGAGAAEKGELAVMVGAERSVYERVKPAFKQWASVVVHAGEPGAGTRMKLARNMLTFTGF
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHH
AAACEAMTLAEKAGLDLQALGRVVRHSDAQSGGPGAIIVRENMRPLDHDNFLYDMFLHTR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHC
GLAEKDLGLALTLGEELDVDLPLAQVALQNLAAGLGVPHVESSTEE
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDLKYGYIGLGNMGAPMAKRLTGWPGGLIVFDVRPDAMAPLVEAGAAQADNVADVADAD
CCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHCCCC
IISITVLNDEQVRHVVAELVPRAKPGTVIAIHSTISPDTAVELAKELRSQDIHVVDAPVS
EEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCC
GGAGAAEKGELAVMVGAERSVYERVKPAFKQWASVVVHAGEPGAGTRMKLARNMLTFTGF
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHH
AAACEAMTLAEKAGLDLQALGRVVRHSDAQSGGPGAIIVRENMRPLDHDNFLYDMFLHTR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHC
GLAEKDLGLALTLGEELDVDLPLAQVALQNLAAGLGVPHVESSTEE
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]