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The map label for this gene is purB [H]

Identifier: 108801529

GI number: 108801529

Start: 4842634

End: 4844067

Strand: Reverse

Name: purB [H]

Synonym: Mmcs_4566

Alternate gene names: 108801529

Gene position: 4844067-4842634 (Counterclockwise)

Preceding gene: 108801531

Following gene: 108801528

Centisome position: 84.9

GC content: 69.32

Gene sequence:

>1434_bases
GTGTCTGTGACGATCCCGAACGTGCTGGCCAACCGCTACGCCAGCGAGGACATGGTGGCGATCTGGTCGCCGGAAGCCAA
GATCGTCGCCGAGCGCCGATTGTGGCTGGCAGTGCTGCGTGCGCAGATAGAGCTCGGCATGGGGAACACGGTCGTTCCCG
ACGGGGTGGTCGCCGACTACGAACGCGTCCTCGACCAGGTGGACCTCGCGTCCATCGCGGCGCGCGAACGCGTCACCCGC
CACGACGTGAAGGCCCGCATCGAGGAGTTCAACGCGCTGGCCGGCCACGAACACGTGCACAAGGGCATGACCAGCCGCGA
CCTCACCGAGAACGTCGAACAGCTACAGATCCGCCGCTCCCTTGAACTCGTCCACGCCCACGGCGTTGCCGTGGTGGCCC
GGCTCGCCGAGAAGGCCGTGAGCTACCGCGACCTCGTGATGGCCGGGCGCAGCCACAACGTCGCCGCACAGGCCACCACA
CTGGGCAAACGGTTCGCCTCCGCCGCCGAGGAGACGTTGCTCGCCCTGCAGCGGGTCGAGGAACTGATCGCGCGTTACCC
GCTGCGCGGTATCAAGGGCCCGATGGGCACCGCCCAGGACATGCTCGACCTGTTCGGCGGCGACACCGCGAAACTGGCCG
ACCTCGAGCGGCGGGTCGCCGAATTCCTCGGCTTCCGAGACGTTTTCACCAGCGTCGGCCAGGTGTACCCCCGTTCCCTC
GACCACGACGTCGTCTCCGCGCTGGTGCAACTCGGCGCGGGTCCCTCCTCGCTCGCGCACACCATCCGGCTGATGGCCGG
CCACGAGCTGGTGACCGAGGGCTTCGCACCGGGCCAGGTCGGATCGTCGGCCATGCCGCACAAGATGAACACCCGGTCCT
GTGAACGCGTCAACGGCCTGCAGGTGGTGCTGCGCGGCTACGGTTCGATGGCCGCCGAACTGGCGGGTGCGCAGTGGAAC
GAAGGCGACGTGTTCTGCTCGGTGGTGCGCCGGGTGGCGCTGCCCGACGCGTTCTTCGCGATCGACGGGCAGACCGAGAC
GTTCCTGACCGTGCTCGACGAGTTCGGCGCCTATCCGGCGGTCATCCAGCGCGAACTGGACCGCTACCTGCCGTTTCTGG
CCACCACCCGCATCCTCATCGCCGCGGTGCGGGCCGGCGTTGGCCGCGAGACCGCGCACGAGGTCATCAAGGAGCACGCG
GTGGCCGTCGCGCTGGCCATGCGGGAGAAGGGGCAGGAGCCCGACCTACTGGACCGGCTGGCTGCCGACCCGCGGCTGCC
GCTGGACCGCGTCGCGCTCGAGGATGCGCTGGCCGACAAGCAGGCGTTCAGCGGGGCCGCCGGCGATCAGGTCGACCGCG
TCGCCCAGGCGGTCGACGAGTTGGTCAGCCGCTATCCGGAGGCGGCCAAGTACACCTCGGGCGCCATCCTGTGA

Upstream 100 bases:

>100_bases
ATCGGCGACGGCGACGCCGGTGGGGCTGCGCTCACCCCACGCGAGGTCGATCCCGACGAAGTACACCTGCCCAGCCTGCC
CGATCCGCGCCGTAAGCTGT

Downstream 100 bases:

>100_bases
GGTTGTCGCAGATCGACTTCACCGATCTGGACAACTTCGCAAACGGGTTCCCTCACGACCTGTTCGCGATCCACCGCCGC
GACGCGCCGGTGTTCTGGCA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSVTIPNVLANRYASEDMVAIWSPEAKIVAERRLWLAVLRAQIELGMGNTVVPDGVVADYERVLDQVDLASIAARERVTR
HDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVHAHGVAVVARLAEKAVSYRDLVMAGRSHNVAAQATT
LGKRFASAAEETLLALQRVEELIARYPLRGIKGPMGTAQDMLDLFGGDTAKLADLERRVAEFLGFRDVFTSVGQVYPRSL
DHDVVSALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYGSMAAELAGAQWN
EGDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILIAAVRAGVGRETAHEVIKEHA
VAVALAMREKGQEPDLLDRLAADPRLPLDRVALEDALADKQAFSGAAGDQVDRVAQAVDELVSRYPEAAKYTSGAIL

Sequences:

>Translated_477_residues
MSVTIPNVLANRYASEDMVAIWSPEAKIVAERRLWLAVLRAQIELGMGNTVVPDGVVADYERVLDQVDLASIAARERVTR
HDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVHAHGVAVVARLAEKAVSYRDLVMAGRSHNVAAQATT
LGKRFASAAEETLLALQRVEELIARYPLRGIKGPMGTAQDMLDLFGGDTAKLADLERRVAEFLGFRDVFTSVGQVYPRSL
DHDVVSALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYGSMAAELAGAQWN
EGDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILIAAVRAGVGRETAHEVIKEHA
VAVALAMREKGQEPDLLDRLAADPRLPLDRVALEDALADKQAFSGAAGDQVDRVAQAVDELVSRYPEAAKYTSGAIL
>Mature_476_residues
SVTIPNVLANRYASEDMVAIWSPEAKIVAERRLWLAVLRAQIELGMGNTVVPDGVVADYERVLDQVDLASIAARERVTRH
DVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVHAHGVAVVARLAEKAVSYRDLVMAGRSHNVAAQATTL
GKRFASAAEETLLALQRVEELIARYPLRGIKGPMGTAQDMLDLFGGDTAKLADLERRVAEFLGFRDVFTSVGQVYPRSLD
HDVVSALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYGSMAAELAGAQWNE
GDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILIAAVRAGVGRETAHEVIKEHAV
AVALAMREKGQEPDLLDRLAADPRLPLDRVALEDALADKQAFSGAAGDQVDRVAQAVDELVSRYPEAAKYTSGAIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=467, Percent_Identity=32.762312633833, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=33.3333333333333, Blast_Score=200, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17508577, Length=398, Percent_Identity=29.1457286432161, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32564234, Length=349, Percent_Identity=31.2320916905444, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323391, Length=452, Percent_Identity=36.0619469026549, Blast_Score=249, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24647570, Length=467, Percent_Identity=33.1905781584582, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51883; Mature: 51752

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTIPNVLANRYASEDMVAIWSPEAKIVAERRLWLAVLRAQIELGMGNTVVPDGVVADY
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ERVLDQVDLASIAARERVTRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHH
LELVHAHGVAVVARLAEKAVSYRDLVMAGRSHNVAAQATTLGKRFASAAEETLLALQRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIARYPLRGIKGPMGTAQDMLDLFGGDTAKLADLERRVAEFLGFRDVFTSVGQVYPRSL
HHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DHDVVSALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGL
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QVVLRGYGSMAAELAGAQWNEGDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHCCCHH
VIQRELDRYLPFLATTRILIAAVRAGVGRETAHEVIKEHAVAVALAMREKGQEPDLLDRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AADPRLPLDRVALEDALADKQAFSGAAGDQVDRVAQAVDELVSRYPEAAKYTSGAIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
SVTIPNVLANRYASEDMVAIWSPEAKIVAERRLWLAVLRAQIELGMGNTVVPDGVVADY
CCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ERVLDQVDLASIAARERVTRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHH
LELVHAHGVAVVARLAEKAVSYRDLVMAGRSHNVAAQATTLGKRFASAAEETLLALQRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIARYPLRGIKGPMGTAQDMLDLFGGDTAKLADLERRVAEFLGFRDVFTSVGQVYPRSL
HHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DHDVVSALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGL
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QVVLRGYGSMAAELAGAQWNEGDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHCCCHH
VIQRELDRYLPFLATTRILIAAVRAGVGRETAHEVIKEHAVAVALAMREKGQEPDLLDRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AADPRLPLDRVALEDALADKQAFSGAAGDQVDRVAQAVDELVSRYPEAAKYTSGAIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]