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The map label for this gene is purF [H]

Identifier: 108801504

GI number: 108801504

Start: 4812866

End: 4814401

Strand: Reverse

Name: purF [H]

Synonym: Mmcs_4541

Alternate gene names: 108801504

Gene position: 4814401-4812866 (Counterclockwise)

Preceding gene: 108801505

Following gene: 108801501

Centisome position: 84.38

GC content: 69.79

Gene sequence:

>1536_bases
GTGAGCGGCCACGACGACATCGAGCCCGAGAACGATCCCCGTGAAGAATGCGGCGTCTTCGGCGTCTGGGCCCCGGGTGA
GGATGTGGCCAAGCTCACCTACTACGGCCTCTATGCGCTACAGCACCGTGGTCAGGAAGCGGCGGGGATCGCCGTAGCCG
ACGGCTCGCAAGTGCTGGTGTTCAAGGATCTCGGCCTGGTCAGCCAGGTGTTCGACGAGCCGACGCTGGCCGCCATGCAC
GGACACGTCGCCATCGGGCACTGCCGCTACTCGACGACCGGGTCGACCACCTGGGAGAACGCCCAGCCCGTCTTCCGCAA
CACCCTGGCCGGCACCGGCGTGGCCGTCGGCCACAACGGCAACCTGGTCAACACCACCGAGCTCGCCGCCCGGGCCCGCG
ACGCCGGCCTGATCAACGGCCGCACCCCCGGCGCGGCGACCACCGATTCGGACATCCTCGGCGCGCTGCTGGCCCACGGC
GCCGCCGACGCCACCGTCGAACAGGCGGCCCTCGAACTGCTGCCGACCGTGCGCGGCGCGTTCTGTCTGACCTTCATGGA
CGAGAACACCCTTTACGCGGCCCGCGACCCGTATGGTGTGCGCCCGCTGTCGCTGGGACGGCTGGACCGGGGCTGGGTGG
TGGCGTCGGAGACCGCGGCCCTCGACATCGTCGGCGCGTCGTTCGTCCGCGACATCGAACCGGGCGAACTGTTGGCCATC
GACGCCGACGGCGTGCGCTCCACCCGCTTCGCCAACCCCACCCCGAAGTCGTGCGTCTTCGAATACGTCTATCTGGCCCG
GCCGGACAGCACGCTGGCCGGCCGCTCGGTGCACGCCACCCGCGTCGACATCGGCCGCCGCCTCGCCAGCGAGATGCCCG
TCGAGGCCGACCTGGTGATCGGGGTCCCCGAGTCGGGCACGCCCGCAGCCGTGGGCTACGCACAGGGCTCCGGCATCCCC
TACGGCCAGGGCCTGATGAAGAACGCCTACGTCGGCCGCACGTTCATCCAGCCGTCGCAGACCATCCGCCAGCTCGGCAT
CCGGCTCAAACTCAACCCGCTCAAAGAGGTCATCCGCGGTAAGCGGTTGATCGTGGTCGACGATTCGATCGTGCGCGGCA
ACACCCAGCGCGCGCTGATCCGGATGCTGCGCGAGGCCGGGGCCCTCGAAGTGCACGTCCGGATCGCCTCCCCGCCGGTG
CGATGGCCGTGCTTCTACGGCATCGACTTCGCCACCCCGGCCGAGCTGATCGCCAACGGCGCCGAGGACGAGGACCAGAT
GCTCGACGCCGTCCGCCGTGCCATCGGCGCGGACACCCTGGGCTACATCTCACAGCAGGGCATGATCGCGGCGACCGAAC
AGCCCGCCTCGCGGCTGTGCTCGGCGTGCTTCGACGGCAACTACCCGATCGAGCTGCCGGGCGAGTCCGCGCTGGGCAAG
AACGTCATCGAGCAGATGCTCGCGACAGCCGCCCGCAGCGGGATCCCGCTCGAGAAGGTCGACAACGACAACGCCTCGGC
GCTTCGTAGGCCTTGA

Upstream 100 bases:

>100_bases
CGAAATAGGTGTTTCACGACACGTCGCCGATTCCCATAACCGGGTATACGGCCCGTAGACTCAACTACGTCACCCACATC
CCCTTGGGAGCAGCCATATC

Downstream 100 bases:

>100_bases
CGCCTCCGGCGTCGTGTAGGCCTTGACGCCTCCGGCGCGTGTAGGCCTTGACGCCTCCTATTGAGCGACCGCGGGATTGA
TCCGCAGCAGGTGACGCCTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSGHDDIEPENDPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEPTLAAMH
GHVAIGHCRYSTTGSTTWENAQPVFRNTLAGTGVAVGHNGNLVNTTELAARARDAGLINGRTPGAATTDSDILGALLAHG
AADATVEQAALELLPTVRGAFCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAI
DADGVRSTRFANPTPKSCVFEYVYLARPDSTLAGRSVHATRVDIGRRLASEMPVEADLVIGVPESGTPAAVGYAQGSGIP
YGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALIRMLREAGALEVHVRIASPPV
RWPCFYGIDFATPAELIANGAEDEDQMLDAVRRAIGADTLGYISQQGMIAATEQPASRLCSACFDGNYPIELPGESALGK
NVIEQMLATAARSGIPLEKVDNDNASALRRP

Sequences:

>Translated_511_residues
MSGHDDIEPENDPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEPTLAAMH
GHVAIGHCRYSTTGSTTWENAQPVFRNTLAGTGVAVGHNGNLVNTTELAARARDAGLINGRTPGAATTDSDILGALLAHG
AADATVEQAALELLPTVRGAFCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAI
DADGVRSTRFANPTPKSCVFEYVYLARPDSTLAGRSVHATRVDIGRRLASEMPVEADLVIGVPESGTPAAVGYAQGSGIP
YGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALIRMLREAGALEVHVRIASPPV
RWPCFYGIDFATPAELIANGAEDEDQMLDAVRRAIGADTLGYISQQGMIAATEQPASRLCSACFDGNYPIELPGESALGK
NVIEQMLATAARSGIPLEKVDNDNASALRRP
>Mature_510_residues
SGHDDIEPENDPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEPTLAAMHG
HVAIGHCRYSTTGSTTWENAQPVFRNTLAGTGVAVGHNGNLVNTTELAARARDAGLINGRTPGAATTDSDILGALLAHGA
ADATVEQAALELLPTVRGAFCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAID
ADGVRSTRFANPTPKSCVFEYVYLARPDSTLAGRSVHATRVDIGRRLASEMPVEADLVIGVPESGTPAAVGYAQGSGIPY
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALIRMLREAGALEVHVRIASPPVR
WPCFYGIDFATPAELIANGAEDEDQMLDAVRRAIGADTLGYISQQGMIAATEQPASRLCSACFDGNYPIELPGESALGKN
VIEQMLATAARSGIPLEKVDNDNASALRRP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=41.4258188824663, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=477, Percent_Identity=38.7840670859539, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=492, Percent_Identity=38.0081300813008, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=484, Percent_Identity=34.7107438016529, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24659598, Length=503, Percent_Identity=40.7554671968191, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI28573187, Length=508, Percent_Identity=40.748031496063, Blast_Score=347, Evalue=9e-96,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=41.0041841004184, Blast_Score=344, Evalue=8e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54459; Mature: 54328

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHDDIEPENDPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLV
CCCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEE
FKDLGLVSQVFDEPTLAAMHGHVAIGHCRYSTTGSTTWENAQPVFRNTLAGTGVAVGHNG
EECHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEECCCC
NLVNTTELAARARDAGLINGRTPGAATTDSDILGALLAHGAADATVEQAALELLPTVRGA
CEEEHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCE
FCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAI
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEE
DADGVRSTRFANPTPKSCVFEYVYLARPDSTLAGRSVHATRVDIGRRLASEMPVEADLVI
ECCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEE
GVPESGTPAAVGYAQGSGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRG
ECCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCEECCHHHHHHHCCCEEEECHHHHHHCC
KRLIVVDDSIVRGNTQRALIRMLREAGALEVHVRIASPPVRWPCFYGIDFATPAELIANG
CEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCC
AEDEDQMLDAVRRAIGADTLGYISQQGMIAATEQPASRLCSACFDGNYPIELPGESALGK
CCCHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEECCCCHHHHH
NVIEQMLATAARSGIPLEKVDNDNASALRRP
HHHHHHHHHHHHCCCCHHHCCCCCCHHCCCC
>Mature Secondary Structure 
SGHDDIEPENDPREECGVFGVWAPGEDVAKLTYYGLYALQHRGQEAAGIAVADGSQVLV
CCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEE
FKDLGLVSQVFDEPTLAAMHGHVAIGHCRYSTTGSTTWENAQPVFRNTLAGTGVAVGHNG
EECHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEECCCC
NLVNTTELAARARDAGLINGRTPGAATTDSDILGALLAHGAADATVEQAALELLPTVRGA
CEEEHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCE
FCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAALDIVGASFVRDIEPGELLAI
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHCCCCCCEEEE
DADGVRSTRFANPTPKSCVFEYVYLARPDSTLAGRSVHATRVDIGRRLASEMPVEADLVI
ECCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEE
GVPESGTPAAVGYAQGSGIPYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRG
ECCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCEECCHHHHHHHCCCEEEECHHHHHHCC
KRLIVVDDSIVRGNTQRALIRMLREAGALEVHVRIASPPVRWPCFYGIDFATPAELIANG
CEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCC
AEDEDQMLDAVRRAIGADTLGYISQQGMIAATEQPASRLCSACFDGNYPIELPGESALGK
CCCHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEECCCCHHHHH
NVIEQMLATAARSGIPLEKVDNDNASALRRP
HHHHHHHHHHHHCCCCHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]