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The map label for this gene is ydaP [H]

Identifier: 108801304

GI number: 108801304

Start: 4616352

End: 4618076

Strand: Reverse

Name: ydaP [H]

Synonym: Mmcs_4340

Alternate gene names: 108801304

Gene position: 4618076-4616352 (Counterclockwise)

Preceding gene: 108801305

Following gene: 108801303

Centisome position: 80.94

GC content: 70.38

Gene sequence:

>1725_bases
ATGACAGATCTGGTTGCGGATGCATTGGTGGCCCGGCTGCGCGACTGGGGCGCCGACCGGGTGTTCGGATATGCCGGCGA
CGGGGTGAACACCCTCCTGGGTGCGTTACAACGTTCGGGTGCACCGGAATTCATCTCCACCCGGCACGAGGAACTCGCGG
CGTTCATGGCCTGCGGACACGCCAAGTACACCGGCCGGGTCGGCGTGTGCATGGCCACCCAGGGCCCCGGCGCCATCCAT
CTGCTGGCCGGCCTCTACGACGCCAAGCTCGACAAGCGGCCCGTGGTGGCGATCGTCGGACAGGTGGTCTCGACCGCGCT
GGGCAGCGGCTACCTGCAGGAGGTCGACCTGCACACGCTCTTCAAAGACGTCTGCGGACAGTACGTGCAGACGGTGTTCG
CACCCGAGCAGGCGCTGATGGCGCTCGACAACGCGATGCGGACCGCGATCGCGACGTCGACACCGACCTGCCTGATCATC
CCGCACGACGTGCAGCAGGCCGAGATGCCCGACGAACTCGCGCACAGCCACGGCGTGGTGCCCTCGGCGGCGGTCAGCGC
GCCGACCCGGATCACCGCCCCCGACGACCAGATCCGCTCGGCCGCCGACATACTCAACGCCGGTCGGCGGGTGGCGTTGC
TCGTGGGTCGCGGCGCCGCCGGTGCGGCCGACGAGATCGCCCGCGTGGTCGAGACACTCGGGGCCGGGGTGACGACGTCA
CTGCTCGGCAAGCCGGTGCTCGACGAGGGGCAGCCGTGGCACACCGGCGTGATGGGTCACCTCGGCACCACCGCGAGCGC
CGAGCTGATGGCGAACTGCGACACCCTGCTGATCGTCGGCTCCAACGATCCCTGGACGGAGTTCTACCCCGCACCCGGAC
AGGCCAAGGCCGTGCAGATCGACATCCAGCCGCGGGTCATCGGTGCGAAGTATCCCGTCGACGCACCCGTCGTCGGGCAA
GCGGCACAGGCACTTTCGGCTCTCTCAGCGCTGCTCGAACCCAAACCGGACCGGGCCTGGCAGGAGCAGGTCCGCCAGTG
GCGTGAGCAGTGGCACGCCGAGGCCGCGCGCCGGGCCGCCGCCGAGACCTCCGACGCCAACCCCGAGGCCGTGGTGCGGG
CACTGTCAGACCATCTGCCCGCCGACGCGCGCGTCGCCGTCGACACCGGATCGTCGACCTACTGGTATGCGCGACACCTC
CGGTTGCCGCCGGGCGTACCCGCCCACCACTCGGGCTATCTCGCGTCGATGGGATGCGCGCTGCCCTACGGGGTGGCCGC
GAAACTCGATGCGCCACAACGGCCGGTGGTGGCGCTGGTCGGTGACGGGGCGATGCAGATGAGCGGACTGCTCGAACTCG
TCACGATCGCCGACCGGTGGCGCTCGTGGCAGGATCCGCGGTTCGTGGTGCTGGTGCTGCACAACGCCGACCTGACCGAG
GTGTCGTGGGAGCAGCGGGAGATGGAGGGCAACCCGCGCTTCGCCCCGTCGCAGGACGTGCCGCGGTTCCCGTACGCCGG
CTACGCCGAACTGCTCGGACTGCGGGGTATCCGGGTGACCTCGTCGCAGGAGGCCGACGACGCCTGGGCGACGGCGTTCT
CCGCCGACCGGCCGACGGTCATCGAGGCGGTGGTCGACGCCGCCACCCCGCTGCTGCCACCGCTGATGCCGGACAGCAAG
GCCGACAAGGTGCGGGCGGCGCTCGAACAGGAGGCCCGAACGTGA

Upstream 100 bases:

>100_bases
TGATCTCCGGCCTGGCGGGCGGGGTGTTCGCCGTGATCGTCGCGGTGTTCGCCGTGCGGCACCGCTGATTGGCGATCGTC
CCGGCGGGTATGCCGCGACG

Downstream 100 bases:

>100_bases
GCCTGTGCCTGGGAATTCGGCCGAATCTCGAACACAGCTCACGTTCGCGCGAGTTTGGGACACGGCCGTCCGGGTATCGA
CTGAGGCATGGCCGAAGCTG

Product: thiamine pyrophosphate enzyme-like TPP binding region

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MTDLVADALVARLRDWGADRVFGYAGDGVNTLLGALQRSGAPEFISTRHEELAAFMACGHAKYTGRVGVCMATQGPGAIH
LLAGLYDAKLDKRPVVAIVGQVVSTALGSGYLQEVDLHTLFKDVCGQYVQTVFAPEQALMALDNAMRTAIATSTPTCLII
PHDVQQAEMPDELAHSHGVVPSAAVSAPTRITAPDDQIRSAADILNAGRRVALLVGRGAAGAADEIARVVETLGAGVTTS
LLGKPVLDEGQPWHTGVMGHLGTTASAELMANCDTLLIVGSNDPWTEFYPAPGQAKAVQIDIQPRVIGAKYPVDAPVVGQ
AAQALSALSALLEPKPDRAWQEQVRQWREQWHAEAARRAAAETSDANPEAVVRALSDHLPADARVAVDTGSSTYWYARHL
RLPPGVPAHHSGYLASMGCALPYGVAAKLDAPQRPVVALVGDGAMQMSGLLELVTIADRWRSWQDPRFVVLVLHNADLTE
VSWEQREMEGNPRFAPSQDVPRFPYAGYAELLGLRGIRVTSSQEADDAWATAFSADRPTVIEAVVDAATPLLPPLMPDSK
ADKVRAALEQEART

Sequences:

>Translated_574_residues
MTDLVADALVARLRDWGADRVFGYAGDGVNTLLGALQRSGAPEFISTRHEELAAFMACGHAKYTGRVGVCMATQGPGAIH
LLAGLYDAKLDKRPVVAIVGQVVSTALGSGYLQEVDLHTLFKDVCGQYVQTVFAPEQALMALDNAMRTAIATSTPTCLII
PHDVQQAEMPDELAHSHGVVPSAAVSAPTRITAPDDQIRSAADILNAGRRVALLVGRGAAGAADEIARVVETLGAGVTTS
LLGKPVLDEGQPWHTGVMGHLGTTASAELMANCDTLLIVGSNDPWTEFYPAPGQAKAVQIDIQPRVIGAKYPVDAPVVGQ
AAQALSALSALLEPKPDRAWQEQVRQWREQWHAEAARRAAAETSDANPEAVVRALSDHLPADARVAVDTGSSTYWYARHL
RLPPGVPAHHSGYLASMGCALPYGVAAKLDAPQRPVVALVGDGAMQMSGLLELVTIADRWRSWQDPRFVVLVLHNADLTE
VSWEQREMEGNPRFAPSQDVPRFPYAGYAELLGLRGIRVTSSQEADDAWATAFSADRPTVIEAVVDAATPLLPPLMPDSK
ADKVRAALEQEART
>Mature_573_residues
TDLVADALVARLRDWGADRVFGYAGDGVNTLLGALQRSGAPEFISTRHEELAAFMACGHAKYTGRVGVCMATQGPGAIHL
LAGLYDAKLDKRPVVAIVGQVVSTALGSGYLQEVDLHTLFKDVCGQYVQTVFAPEQALMALDNAMRTAIATSTPTCLIIP
HDVQQAEMPDELAHSHGVVPSAAVSAPTRITAPDDQIRSAADILNAGRRVALLVGRGAAGAADEIARVVETLGAGVTTSL
LGKPVLDEGQPWHTGVMGHLGTTASAELMANCDTLLIVGSNDPWTEFYPAPGQAKAVQIDIQPRVIGAKYPVDAPVVGQA
AQALSALSALLEPKPDRAWQEQVRQWREQWHAEAARRAAAETSDANPEAVVRALSDHLPADARVAVDTGSSTYWYARHLR
LPPGVPAHHSGYLASMGCALPYGVAAKLDAPQRPVVALVGDGAMQMSGLLELVTIADRWRSWQDPRFVVLVLHNADLTEV
SWEQREMEGNPRFAPSQDVPRFPYAGYAELLGLRGIRVTSSQEADDAWATAFSADRPTVIEAVVDAATPLLPPLMPDSKA
DKVRAALEQEART

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=22.0665499124343, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=31.9569120287253, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.6219081272085, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=24.3291592128801, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786717, Length=586, Percent_Identity=25.4266211604096, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=23.9285714285714, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=23.5191637630662, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=23.5191637630662, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=26.643598615917, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=572, Percent_Identity=22.7272727272727, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922626, Length=580, Percent_Identity=24.4827586206897, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 61179; Mature: 61048

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLVADALVARLRDWGADRVFGYAGDGVNTLLGALQRSGAPEFISTRHEELAAFMACGH
CCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
AKYTGRVGVCMATQGPGAIHLLAGLYDAKLDKRPVVAIVGQVVSTALGSGYLQEVDLHTL
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
FKDVCGQYVQTVFAPEQALMALDNAMRTAIATSTPTCLIIPHDVQQAEMPDELAHSHGVV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHHHCCCC
PSAAVSAPTRITAPDDQIRSAADILNAGRRVALLVGRGAAGAADEIARVVETLGAGVTTS
CCHHCCCCCEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHH
LLGKPVLDEGQPWHTGVMGHLGTTASAELMANCDTLLIVGSNDPWTEFYPAPGQAKAVQI
HHCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
DIQPRVIGAKYPVDAPVVGQAAQALSALSALLEPKPDRAWQEQVRQWREQWHAEAARRAA
EECCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AETSDANPEAVVRALSDHLPADARVAVDTGSSTYWYARHLRLPPGVPAHHSGYLASMGCA
HCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHCCC
LPYGVAAKLDAPQRPVVALVGDGAMQMSGLLELVTIADRWRSWQDPRFVVLVLHNADLTE
CCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
VSWEQREMEGNPRFAPSQDVPRFPYAGYAELLGLRGIRVTSSQEADDAWATAFSADRPTV
CCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCHH
IEAVVDAATPLLPPLMPDSKADKVRAALEQEART
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDLVADALVARLRDWGADRVFGYAGDGVNTLLGALQRSGAPEFISTRHEELAAFMACGH
CHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
AKYTGRVGVCMATQGPGAIHLLAGLYDAKLDKRPVVAIVGQVVSTALGSGYLQEVDLHTL
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
FKDVCGQYVQTVFAPEQALMALDNAMRTAIATSTPTCLIIPHDVQQAEMPDELAHSHGVV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHHHCCCC
PSAAVSAPTRITAPDDQIRSAADILNAGRRVALLVGRGAAGAADEIARVVETLGAGVTTS
CCHHCCCCCEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHH
LLGKPVLDEGQPWHTGVMGHLGTTASAELMANCDTLLIVGSNDPWTEFYPAPGQAKAVQI
HHCCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
DIQPRVIGAKYPVDAPVVGQAAQALSALSALLEPKPDRAWQEQVRQWREQWHAEAARRAA
EECCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AETSDANPEAVVRALSDHLPADARVAVDTGSSTYWYARHLRLPPGVPAHHSGYLASMGCA
HCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHCCC
LPYGVAAKLDAPQRPVVALVGDGAMQMSGLLELVTIADRWRSWQDPRFVVLVLHNADLTE
CCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
VSWEQREMEGNPRFAPSQDVPRFPYAGYAELLGLRGIRVTSSQEADDAWATAFSADRPTV
CCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCHH
IEAVVDAATPLLPPLMPDSKADKVRAALEQEART
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]