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The map label for this gene is eno [H]

Identifier: 108801204

GI number: 108801204

Start: 4502145

End: 4503434

Strand: Reverse

Name: eno [H]

Synonym: Mmcs_4240

Alternate gene names: 108801204

Gene position: 4503434-4502145 (Counterclockwise)

Preceding gene: 108801205

Following gene: 108801203

Centisome position: 78.93

GC content: 69.3

Gene sequence:

>1290_bases
GTGCCCATCATCGAGCAGGTCGGAGCCCGCGAGATCCTCGACTCCCGCGGCAACCCGACGGTCGAGGTCGAGTTGGCGCT
GACCGACGGCACCTTCGCCCGGGCCGCGGTGCCCTCGGGCGCGTCGACCGGTGAGCACGAGGCCGTCGAACTGCGCGACG
GTGGATCTCGCTACGGCGGCAAGGGCGTCGACAAGGCCGTGCAGGCCGTGCTCGACGACATCGCGCCCGCGGTGATCGGC
ATGAGCGCCGACGATCAGCGGCTCATCGACCAGGCCCTGCTCGACCTCGACGGCACCCCGGACAAGTCCCGGTTGGGCGC
CAACGCGATCCTCGGGGTGTCGCTGGCGGTGTCGAAGGCCGCGGCCGAATCGGCCGGTCTGCCCCTTTTTCGGTACCTGG
GCGGACCCAATGCCCACATCCTGCCGGTGCCGATGATGAACATCCTCAACGGTGGCGCGCACGCCGACACCGGCGTCGAC
GTCCAGGAGTTCATGGTCGCCCCGATCGGTGCCCCGTCGTTCAAGGAGGCGCTGCGCTGGGGCGCCGAGGTGTACCACTC
GCTCAAGTCGGTGCTCAAGAAGCAGGGACTGTCGACCGGGCTGGGTGACGAGGGCGGTTTCGCGCCCGACGTGGCGGGCA
CCAAGGCCGCGCTGGACCTGATCTCGTCGGCGATCGAGGCCGCGGGCTTCAAGCTCGGCACCGATGTGACGCTGGCGCTC
GACGTCGCGGCCACCGAGTTCTACACCGAGGGCACGGGATACAGCTTCGAGAAGGAGACCCGCACCGCCGAGCAGATGGC
CGAGTTCTACGCCTCGCTGCTGGACGCCTACCCGCTGGTGTCGATCGAGGATCCGCTGTCCGAGGACGACTGGGACGGCT
GGGTGTCGCTGACGACGCAGATCGGCGACCGGGTGCAGCTGGTCGGCGACGATCTGTTCGTCACCAACCCCGAGCGCCTC
GAAGAGGGCATCGAACGCGGCGCGGCCAACGCGCTGCTGGTGAAGGTCAACCAGATCGGCACCCTCACCGAGACGCTCGA
CGCCGTCGCGCTGGCCCACAACAGCGGGTACCGCACGATGATGAGCCACCGCAGCGGTGAGACCGAGGACACCACGATCG
CCGACCTCGCCGTCGCGGTCGGCAGCGGTCAGATCAAGACCGGCGCACCGGCGCGCAGCGAGCGCGTCGCCAAGTACAAC
CAGCTGCTGCGCATCGAGGAGACCCTCGGTGACGCCGCCCGTTACGCCGGCGACCTGGCCTTCCCGCGCTTCGCCCTGGA
GACCAAATAG

Upstream 100 bases:

>100_bases
CCGACGGCCGCCGACTCTAAACCGGCCTCTGGTCACGCCGTGGCAGCAGGCAGCCTTTAGGCTGAGGCGACGTCCGCACA
GCCCCCCGAAGGAGACGCCA

Downstream 100 bases:

>100_bases
GCATGGTGCGCTGAAAGACGATGCCCGAATCGAAGCGGCCCGATCCGAAACGTCGGTCCCCGGCCTCCCGGCCGGGTAAG
CCCGGCGGTGCGAACCGGGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTGEHEAVELRDGGSRYGGKGVDKAVQAVLDDIAPAVIG
MSADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVSKAAAESAGLPLFRYLGGPNAHILPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPSFKEALRWGAEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTKAALDLISSAIEAAGFKLGTDVTLAL
DVAATEFYTEGTGYSFEKETRTAEQMAEFYASLLDAYPLVSIEDPLSEDDWDGWVSLTTQIGDRVQLVGDDLFVTNPERL
EEGIERGAANALLVKVNQIGTLTETLDAVALAHNSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEETLGDAARYAGDLAFPRFALETK

Sequences:

>Translated_429_residues
MPIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTGEHEAVELRDGGSRYGGKGVDKAVQAVLDDIAPAVIG
MSADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVSKAAAESAGLPLFRYLGGPNAHILPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPSFKEALRWGAEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTKAALDLISSAIEAAGFKLGTDVTLAL
DVAATEFYTEGTGYSFEKETRTAEQMAEFYASLLDAYPLVSIEDPLSEDDWDGWVSLTTQIGDRVQLVGDDLFVTNPERL
EEGIERGAANALLVKVNQIGTLTETLDAVALAHNSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEETLGDAARYAGDLAFPRFALETK
>Mature_428_residues
PIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTGEHEAVELRDGGSRYGGKGVDKAVQAVLDDIAPAVIGM
SADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVSKAAAESAGLPLFRYLGGPNAHILPVPMMNILNGGAHADTGVDV
QEFMVAPIGAPSFKEALRWGAEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTKAALDLISSAIEAAGFKLGTDVTLALD
VAATEFYTEGTGYSFEKETRTAEQMAEFYASLLDAYPLVSIEDPLSEDDWDGWVSLTTQIGDRVQLVGDDLFVTNPERLE
EGIERGAANALLVKVNQIGTLTETLDAVALAHNSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQ
LLRIEETLGDAARYAGDLAFPRFALETK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=53.953488372093, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=53.953488372093, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=54.9065420560748, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=52.3255813953488, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=50.2336448598131, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=56.4705882352941, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.6558891454965, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.6558891454965, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=51.0526315789474, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=53.2407407407407, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=50.8120649651972, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=50.8120649651972, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=50.8120649651972, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=52.0833333333333, Blast_Score=386, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=52.2144522144522, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45164; Mature: 45033

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTGEHEAVELRDGGSRYGG
CCCHHHCCHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCCEEEECCCCCCCCC
KGVDKAVQAVLDDIAPAVIGMSADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVSKA
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHH
AAESAGLPLFRYLGGPNAHILPVPMMNILNGGAHADTGVDVQEFMVAPIGAPSFKEALRW
HHHHCCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTKAALDLISSAIEAAGFKLGTDVTLAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVAATEFYTEGTGYSFEKETRTAEQMAEFYASLLDAYPLVSIEDPLSEDDWDGWVSLTTQ
EEHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHEEEHHH
IGDRVQLVGDDLFVTNPERLEEGIERGAANALLVKVNQIGTLTETLDAVALAHNSGYRTM
HCCEEEEECCCEEECCHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHCCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEETLGDAARYAGDLA
HHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPRFALETK
CCCEECCCC
>Mature Secondary Structure 
PIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTGEHEAVELRDGGSRYGG
CCHHHCCHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCCEEEECCCCCCCCC
KGVDKAVQAVLDDIAPAVIGMSADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVSKA
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHH
AAESAGLPLFRYLGGPNAHILPVPMMNILNGGAHADTGVDVQEFMVAPIGAPSFKEALRW
HHHHCCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTKAALDLISSAIEAAGFKLGTDVTLAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVAATEFYTEGTGYSFEKETRTAEQMAEFYASLLDAYPLVSIEDPLSEDDWDGWVSLTTQ
EEHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHEEEHHH
IGDRVQLVGDDLFVTNPERLEEGIERGAANALLVKVNQIGTLTETLDAVALAHNSGYRTM
HCCEEEEECCCEEECCHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHCCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEETLGDAARYAGDLA
HHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPRFALETK
CCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA