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The map label for this gene is fadD21 [H]

Identifier: 108800833

GI number: 108800833

Start: 4116128

End: 4117945

Strand: Reverse

Name: fadD21 [H]

Synonym: Mmcs_3869

Alternate gene names: 108800833

Gene position: 4117945-4116128 (Counterclockwise)

Preceding gene: 108800836

Following gene: 108800831

Centisome position: 72.18

GC content: 70.57

Gene sequence:

>1818_bases
ATGAGCGGCGGTACCGGGCAGATGGTTCACGAAGGTCTGCTGAAGATCGAGGACTGCCTCGACGCCGCGGGCAACATCGT
GCTCCCGCCGGGCGTCACGCTGATCTCGCTCATCGAGCGCAACATCGCCGCCGTCGGCGACGCCGTGGCGTACCGCTACC
TCGACCACACCGGCACCGACGAGGTGCGGGTCGCGGAGCTCACCTGGACCGAACTCGGCGTGCGGTTACGCGCGGTCGGT
GCGCGGATACAGCAGCTCACCTCCCGCGGCGACCGCGTCGCGATCCTCGCGCCCCAGGGCCTGGACTGGGTCACCGGGTT
CTTCGCCGCGATCAAGGCGGGCACCATCGCGGTGCCGCTGTTCGCGCCCGAACTGCAGGGCCACGCCGAACGCCTCCGGA
CCGCGCTCGAGGACGCCCGCCCGACCGTCGTGCTCACCACCTCGGCGGCGGCCGGCGCCGTCGAAGGTTTCCTCGCCGAC
CTCGTGGGCGTCACGCGTCCGCACGTCGTGCTGATCGACGACATTCCCGACTCCGCGGGGGAGTCGTTCGTGACCACGCC
GATCGACGTCGACGACGTCTCGCACCTGCAGTACACCTCCGGCGCGACCCGGCCCCCGGCCGGCGTCGAGATCACCCACC
GTGCCGTCGGCACGAACCTCCTGCAGATGATCCTGTCGATCGACCTGCTGGACCGAAACACCCACGGCGTCAGTTGGTTA
CCGCTGTTCCACGACATGGGGCTGTCGATGATCGGCTTCCCGGCCGTCTACGGCGGGCACTCCACGCTGATGTCGCCGAC
GGCGTTCCTGCGCCGGCCCCAGCGCTGGATCCGCGCGCTGTCGGAGGGGTCCCGGCAGGGCCGCGTCGTCACCGCCGCGC
CGAACTTCGCCTTCGAGTGGACCGCCCAGCGCGGCTTGCCCGACGAAGGTGAGGACATCGATCTGGGCAACGTCGTCCTC
ATCGTCGGATCCGAACCGGTGAGCATGGCGGCCATCGACACCTTCACCGCGGCCTTCGCCGAATACGGGCTGCCGCCGAC
GGCGGTCAAACCGTCCTACGGCATCGCCGAGGCGACGCTGTTCGTCGCGACGATCGCCCCGGACGCGGAACCGTCGGTGG
CGCACTTCGACCGCGCACAACTGGCCGCAGGCCGGGCGGTCCGCGTGGCCGCCGACGCGCAGGACGCGGTGGCCCAGGTG
TCGTGCGGTCAGATCGCCCGGAGCCAGTGGGCGGTGATCGTCGACCCCGACTCCGGTGAAGAGATGCCCGACGGATATGT
CGGCGAGATCTGGTTGCACGGTGAGAACCTCGGCCGCGGATACTGGGGACGACCCGACGAGACTCGCGAGACATTCTGCG
CCGCAATGACATCGAGGGCGGGAGGTCATGCCGACGGCGTTCCCGACGACGGTCGCTGGCTGCGCACGGGCGACTTGGGC
ACCTATCTCGACGGCGAACTCTATGTCACCGGCCGGCTGGCGGACCTCATCGTGATCGACGGGCGCGGGCACTATCCGCA
GGACATCGAGGCCACCACAGCGGACGCCTCGCCGCTGGTGCGCCGCGGCCACGCCACCGCGTTCACCGTCTCCGGCGAGC
AGTTGGTGATCATCGCCGAGCGCGCATCGGGGACCCGCCGCGCCGACCCGGCGCCTGCCGTCGAGGCGATCCGCGCCGCG
GTGCGGCAGCGCCACGGAATCGAGGTCGCCGACGTGCGTTTCGTCCCCGCCGGCGCCATCCCGCGGACAACCAGCGGCAA
GCTGGCCCGACGGGCCTGTCGCACCGAATACATCGACGGAACGCTGCGCGCGAACTAG

Upstream 100 bases:

>100_bases
ATGCTGTCAGACTTACACGCCGCCACCGACACCACCGGCGGAAATCCGACATGGTCGTGCGCCATGCTGCCCGTGCCGCC
GCATCCCTAGACTCGAAACC

Downstream 100 bases:

>100_bases
GCGTCAGCCAGCAGCGCCAGCGTCGTCGGCTCGTCGTGGCCGCGCAGGGTCACCGTGTCGCCGAGAGTCCAGTGCGCGCG
TTCGGCATCCGTCGCGGCCT

Product: putative fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MSGGTGQMVHEGLLKIEDCLDAAGNIVLPPGVTLISLIERNIAAVGDAVAYRYLDHTGTDEVRVAELTWTELGVRLRAVG
ARIQQLTSRGDRVAILAPQGLDWVTGFFAAIKAGTIAVPLFAPELQGHAERLRTALEDARPTVVLTTSAAAGAVEGFLAD
LVGVTRPHVVLIDDIPDSAGESFVTTPIDVDDVSHLQYTSGATRPPAGVEITHRAVGTNLLQMILSIDLLDRNTHGVSWL
PLFHDMGLSMIGFPAVYGGHSTLMSPTAFLRRPQRWIRALSEGSRQGRVVTAAPNFAFEWTAQRGLPDEGEDIDLGNVVL
IVGSEPVSMAAIDTFTAAFAEYGLPPTAVKPSYGIAEATLFVATIAPDAEPSVAHFDRAQLAAGRAVRVAADAQDAVAQV
SCGQIARSQWAVIVDPDSGEEMPDGYVGEIWLHGENLGRGYWGRPDETRETFCAAMTSRAGGHADGVPDDGRWLRTGDLG
TYLDGELYVTGRLADLIVIDGRGHYPQDIEATTADASPLVRRGHATAFTVSGEQLVIIAERASGTRRADPAPAVEAIRAA
VRQRHGIEVADVRFVPAGAIPRTTSGKLARRACRTEYIDGTLRAN

Sequences:

>Translated_605_residues
MSGGTGQMVHEGLLKIEDCLDAAGNIVLPPGVTLISLIERNIAAVGDAVAYRYLDHTGTDEVRVAELTWTELGVRLRAVG
ARIQQLTSRGDRVAILAPQGLDWVTGFFAAIKAGTIAVPLFAPELQGHAERLRTALEDARPTVVLTTSAAAGAVEGFLAD
LVGVTRPHVVLIDDIPDSAGESFVTTPIDVDDVSHLQYTSGATRPPAGVEITHRAVGTNLLQMILSIDLLDRNTHGVSWL
PLFHDMGLSMIGFPAVYGGHSTLMSPTAFLRRPQRWIRALSEGSRQGRVVTAAPNFAFEWTAQRGLPDEGEDIDLGNVVL
IVGSEPVSMAAIDTFTAAFAEYGLPPTAVKPSYGIAEATLFVATIAPDAEPSVAHFDRAQLAAGRAVRVAADAQDAVAQV
SCGQIARSQWAVIVDPDSGEEMPDGYVGEIWLHGENLGRGYWGRPDETRETFCAAMTSRAGGHADGVPDDGRWLRTGDLG
TYLDGELYVTGRLADLIVIDGRGHYPQDIEATTADASPLVRRGHATAFTVSGEQLVIIAERASGTRRADPAPAVEAIRAA
VRQRHGIEVADVRFVPAGAIPRTTSGKLARRACRTEYIDGTLRAN
>Mature_604_residues
SGGTGQMVHEGLLKIEDCLDAAGNIVLPPGVTLISLIERNIAAVGDAVAYRYLDHTGTDEVRVAELTWTELGVRLRAVGA
RIQQLTSRGDRVAILAPQGLDWVTGFFAAIKAGTIAVPLFAPELQGHAERLRTALEDARPTVVLTTSAAAGAVEGFLADL
VGVTRPHVVLIDDIPDSAGESFVTTPIDVDDVSHLQYTSGATRPPAGVEITHRAVGTNLLQMILSIDLLDRNTHGVSWLP
LFHDMGLSMIGFPAVYGGHSTLMSPTAFLRRPQRWIRALSEGSRQGRVVTAAPNFAFEWTAQRGLPDEGEDIDLGNVVLI
VGSEPVSMAAIDTFTAAFAEYGLPPTAVKPSYGIAEATLFVATIAPDAEPSVAHFDRAQLAAGRAVRVAADAQDAVAQVS
CGQIARSQWAVIVDPDSGEEMPDGYVGEIWLHGENLGRGYWGRPDETRETFCAAMTSRAGGHADGVPDDGRWLRTGDLGT
YLDGELYVTGRLADLIVIDGRGHYPQDIEATTADASPLVRRGHATAFTVSGEQLVIIAERASGTRRADPAPAVEAIRAAV
RQRHGIEVADVRFVPAGAIPRTTSGKLARRACRTEYIDGTLRAN

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=586, Percent_Identity=24.7440273037543, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI55749758, Length=544, Percent_Identity=23.3455882352941, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI45827692, Length=475, Percent_Identity=25.0526315789474, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI225735629, Length=475, Percent_Identity=25.0526315789474, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI225735625, Length=588, Percent_Identity=22.7891156462585, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193202679, Length=555, Percent_Identity=22.8828828828829, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=572, Percent_Identity=25, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654656, Length=451, Percent_Identity=23.5033259423503, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64415; Mature: 64284

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGTGQMVHEGLLKIEDCLDAAGNIVLPPGVTLISLIERNIAAVGDAVAYRYLDHTGTD
CCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EVRVAELTWTELGVRLRAVGARIQQLTSRGDRVAILAPQGLDWVTGFFAAIKAGTIAVPL
CEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEE
FAPELQGHAERLRTALEDARPTVVLTTSAAAGAVEGFLADLVGVTRPHVVLIDDIPDSAG
ECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ESFVTTPIDVDDVSHLQYTSGATRPPAGVEITHRAVGTNLLQMILSIDLLDRNTHGVSWL
CCEEECCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PLFHDMGLSMIGFPAVYGGHSTLMSPTAFLRRPQRWIRALSEGSRQGRVVTAAPNFAFEW
HHHHHCCCHHHCCCCEECCCHHHCCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCEEEE
TAQRGLPDEGEDIDLGNVVLIVGSEPVSMAAIDTFTAAFAEYGLPPTAVKPSYGIAEATL
ECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
FVATIAPDAEPSVAHFDRAQLAAGRAVRVAADAQDAVAQVSCGQIARSQWAVIVDPDSGE
EEEEECCCCCCCHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCC
EMPDGYVGEIWLHGENLGRGYWGRPDETRETFCAAMTSRAGGHADGVPDDGRWLRTGDLG
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
TYLDGELYVTGRLADLIVIDGRGHYPQDIEATTADASPLVRRGHATAFTVSGEQLVIIAE
CEECCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCEEEEEEE
RASGTRRADPAPAVEAIRAAVRQRHGIEVADVRFVPAGAIPRTTSGKLARRACRTEYIDG
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
TLRAN
EEECC
>Mature Secondary Structure 
SGGTGQMVHEGLLKIEDCLDAAGNIVLPPGVTLISLIERNIAAVGDAVAYRYLDHTGTD
CCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EVRVAELTWTELGVRLRAVGARIQQLTSRGDRVAILAPQGLDWVTGFFAAIKAGTIAVPL
CEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEE
FAPELQGHAERLRTALEDARPTVVLTTSAAAGAVEGFLADLVGVTRPHVVLIDDIPDSAG
ECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ESFVTTPIDVDDVSHLQYTSGATRPPAGVEITHRAVGTNLLQMILSIDLLDRNTHGVSWL
CCEEECCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PLFHDMGLSMIGFPAVYGGHSTLMSPTAFLRRPQRWIRALSEGSRQGRVVTAAPNFAFEW
HHHHHCCCHHHCCCCEECCCHHHCCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCEEEE
TAQRGLPDEGEDIDLGNVVLIVGSEPVSMAAIDTFTAAFAEYGLPPTAVKPSYGIAEATL
ECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
FVATIAPDAEPSVAHFDRAQLAAGRAVRVAADAQDAVAQVSCGQIARSQWAVIVDPDSGE
EEEEECCCCCCCHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCC
EMPDGYVGEIWLHGENLGRGYWGRPDETRETFCAAMTSRAGGHADGVPDDGRWLRTGDLG
CCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
TYLDGELYVTGRLADLIVIDGRGHYPQDIEATTADASPLVRRGHATAFTVSGEQLVIIAE
CEECCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCEEEEEEE
RASGTRRADPAPAVEAIRAAVRQRHGIEVADVRFVPAGAIPRTTSGKLARRACRTEYIDG
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
TLRAN
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]