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The map label for this gene is proP [H]

Identifier: 108800800

GI number: 108800800

Start: 4083384

End: 4084721

Strand: Reverse

Name: proP [H]

Synonym: Mmcs_3836

Alternate gene names: 108800800

Gene position: 4084721-4083384 (Counterclockwise)

Preceding gene: 108800801

Following gene: 108800799

Centisome position: 71.59

GC content: 68.46

Gene sequence:

>1338_bases
GTGCCTCTCGCCATGGAGAGTCAGACATCCGCGCCTGCACCCGCCGAGACGGCGGCCATCCGCAAAGCGGTCGCAGGCGC
GTCGATCGGTAACGCCGTCGAATGGTTCGACTTCGCCATCTACGGCTTCCTCGCCACTTTCATCGCCGCCAAGTTCTTCC
CGGCGGGCAACGACACGGCCGCCCTGCTGAACACGTTCGCGATCTTCGCCGCGGCCTTCTTCATGCGGCCCCTCGGCGGT
TTCGTCTTCGGCCCGCTCGGCGACCGGATCGGACGTCAGCGGGTGCTCGCGGTGGTCATCCTGCTGATGTCGGCGGCCAC
GCTCGGCATCGGCCTGCTGCCGACCTACGCCAGCATCGGCGTCGCCGCACCGCTGCTGCTGCTGTTCCTGCGGTGCCTGC
AGGGGTTCTCCGCGGGCGGTGAATACGGTGGCGGCGCAGTCTATCTCGCCGAGTACGCCCGTGACCGCAACCGCGGGTTG
ACGGTCACGTTCATCGCCTGGTCCGGTGTCGTCGGCTTCCTGCTCGGCTCGGTGACGGTGACGGTGCTGCAGGCGCTGTT
GCCCACCGCGGCGATGGACAGCTACGGCTGGCGGATCCCGTTCCTGATCGCCGCACCGCTGGGCCTGGTCGGCCTCTACA
TCCGGTTACGCCTCGACGACACGCCCGAGTTCGCGGCGCTGTCGGAGACCGACCGGGTGGCGTCGTCGCCGCTGCGTGAG
GCGGCCACCACCGCGTGGCGGCCGATCCTGCAGGTGATCGGCCTGTTCATCGTGTTCAATGTCGGCTACTACGTGGTGTT
CACGTTCCTGCCGACCTACTTCATCAAGACCCTCGAATTCGGCAAGACGGCGGCGTTCGTCTCGATGTCGCTGGCCAGTC
TGGTCGCCCTGGTGCTGATCCTGCCGCTGGCCGCGCTGTCGGACCGCATCGGCAGGCGTCCGCTGCTGATCGGCGGGACG
GTGGCGTTCGTGATCCTCGCGTATCCGCTGTTCCTGCTGCTGAATTCCGGTTCGCTCGCCGCGGCGATCACCGCGCACTG
CGTGCTGGCGGCCATCGAGTCGATCTACGTGTCGACCGCGGTCACCGCCGGCGTCGAACTGTTCGCCACCCGGGTGCGCT
ACAGCGGGTTCTCCATCGGCTACAACGTGTCGGTCGCGGCGTTCGGCGGGACGACGCCCTACGTCGTCACGTGGCTGACC
GCGACGACCGAGAACAACCTCGCCCCGGCGCTGTACCTGATCGTCGCGGCCGTCGTATCGCTGGCGACGCTGCTCACCCT
GCGGGAGTCCGCCGGCCGGCCGCTGGCCGCGACAGTGGCCGCTGACGTGCGACGATGA

Upstream 100 bases:

>100_bases
TCTGGCCGCCGGTCGCGAGATCCCGGCGGACCGGCTGAGCCCCACGCTCGTCGTGCGTACGGACTGAAACGACCACCTAC
TTACGCGGATAAGCGGTAGT

Downstream 100 bases:

>100_bases
GCTGGTGAACGGTAGGGGAACGGCGCGGCCCACCAAGGCCGACGTGGCCCGTCTCGCCAACGTGTCGACGGCCACCGTCA
GCTACGTCCTCAACAACGTC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPLAMESQTSAPAPAETAAIRKAVAGASIGNAVEWFDFAIYGFLATFIAAKFFPAGNDTAALLNTFAIFAAAFFMRPLGG
FVFGPLGDRIGRQRVLAVVILLMSAATLGIGLLPTYASIGVAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEYARDRNRGL
TVTFIAWSGVVGFLLGSVTVTVLQALLPTAAMDSYGWRIPFLIAAPLGLVGLYIRLRLDDTPEFAALSETDRVASSPLRE
AATTAWRPILQVIGLFIVFNVGYYVVFTFLPTYFIKTLEFGKTAAFVSMSLASLVALVLILPLAALSDRIGRRPLLIGGT
VAFVILAYPLFLLLNSGSLAAAITAHCVLAAIESIYVSTAVTAGVELFATRVRYSGFSIGYNVSVAAFGGTTPYVVTWLT
ATTENNLAPALYLIVAAVVSLATLLTLRESAGRPLAATVAADVRR

Sequences:

>Translated_445_residues
MPLAMESQTSAPAPAETAAIRKAVAGASIGNAVEWFDFAIYGFLATFIAAKFFPAGNDTAALLNTFAIFAAAFFMRPLGG
FVFGPLGDRIGRQRVLAVVILLMSAATLGIGLLPTYASIGVAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEYARDRNRGL
TVTFIAWSGVVGFLLGSVTVTVLQALLPTAAMDSYGWRIPFLIAAPLGLVGLYIRLRLDDTPEFAALSETDRVASSPLRE
AATTAWRPILQVIGLFIVFNVGYYVVFTFLPTYFIKTLEFGKTAAFVSMSLASLVALVLILPLAALSDRIGRRPLLIGGT
VAFVILAYPLFLLLNSGSLAAAITAHCVLAAIESIYVSTAVTAGVELFATRVRYSGFSIGYNVSVAAFGGTTPYVVTWLT
ATTENNLAPALYLIVAAVVSLATLLTLRESAGRPLAATVAADVRR
>Mature_444_residues
PLAMESQTSAPAPAETAAIRKAVAGASIGNAVEWFDFAIYGFLATFIAAKFFPAGNDTAALLNTFAIFAAAFFMRPLGGF
VFGPLGDRIGRQRVLAVVILLMSAATLGIGLLPTYASIGVAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEYARDRNRGLT
VTFIAWSGVVGFLLGSVTVTVLQALLPTAAMDSYGWRIPFLIAAPLGLVGLYIRLRLDDTPEFAALSETDRVASSPLREA
ATTAWRPILQVIGLFIVFNVGYYVVFTFLPTYFIKTLEFGKTAAFVSMSLASLVALVLILPLAALSDRIGRRPLLIGGTV
AFVILAYPLFLLLNSGSLAAAITAHCVLAAIESIYVSTAVTAGVELFATRVRYSGFSIGYNVSVAAFGGTTPYVVTWLTA
TTENNLAPALYLIVAAVVSLATLLTLRESAGRPLAATVAADVRR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=442, Percent_Identity=39.3665158371041, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788942, Length=441, Percent_Identity=35.827664399093, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=32.4519230769231, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789941, Length=436, Percent_Identity=34.4036697247706, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87082231, Length=230, Percent_Identity=25.2173913043478, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46912; Mature: 46780

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAMESQTSAPAPAETAAIRKAVAGASIGNAVEWFDFAIYGFLATFIAAKFFPAGNDTA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALLNTFAIFAAAFFMRPLGGFVFGPLGDRIGRQRVLAVVILLMSAATLGIGLLPTYASIG
HHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEYARDRNRGLTVTFIAWSGVVGFLLGSVTV
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
TVLQALLPTAAMDSYGWRIPFLIAAPLGLVGLYIRLRLDDTPEFAALSETDRVASSPLRE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHCCHHHHHHHHHHHH
AATTAWRPILQVIGLFIVFNVGYYVVFTFLPTYFIKTLEFGKTAAFVSMSLASLVALVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LPLAALSDRIGRRPLLIGGTVAFVILAYPLFLLLNSGSLAAAITAHCVLAAIESIYVSTA
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VTAGVELFATRVRYSGFSIGYNVSVAAFGGTTPYVVTWLTATTENNLAPALYLIVAAVVS
HHHHHHHHHHHHHHCCEEECCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
LATLLTLRESAGRPLAATVAADVRR
HHHHHHHHHHCCCCEEHHHHHHCCC
>Mature Secondary Structure 
PLAMESQTSAPAPAETAAIRKAVAGASIGNAVEWFDFAIYGFLATFIAAKFFPAGNDTA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ALLNTFAIFAAAFFMRPLGGFVFGPLGDRIGRQRVLAVVILLMSAATLGIGLLPTYASIG
HHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEYARDRNRGLTVTFIAWSGVVGFLLGSVTV
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
TVLQALLPTAAMDSYGWRIPFLIAAPLGLVGLYIRLRLDDTPEFAALSETDRVASSPLRE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHCCHHHHHHHHHHHH
AATTAWRPILQVIGLFIVFNVGYYVVFTFLPTYFIKTLEFGKTAAFVSMSLASLVALVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LPLAALSDRIGRRPLLIGGTVAFVILAYPLFLLLNSGSLAAAITAHCVLAAIESIYVSTA
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VTAGVELFATRVRYSGFSIGYNVSVAAFGGTTPYVVTWLTATTENNLAPALYLIVAAVVS
HHHHHHHHHHHHHHCCEEECCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
LATLLTLRESAGRPLAATVAADVRR
HHHHHHHHHHCCCCEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]