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The map label for this gene is nadE [H]

Identifier: 108800509

GI number: 108800509

Start: 3770277

End: 3772319

Strand: Reverse

Name: nadE [H]

Synonym: Mmcs_3543

Alternate gene names: 108800509

Gene position: 3772319-3770277 (Counterclockwise)

Preceding gene: 108800513

Following gene: 108800504

Centisome position: 66.12

GC content: 66.28

Gene sequence:

>2043_bases
ATGGATTTTTACTCGGCGTACCGTCACGGCTTCGCGCGGATCGCCGCGTGCACGCACCACACGTCCATCGCGGACCCCAA
GGGCAACGCCGAGTCCGTACTGCGGATGGCGCGTGACTGTCATGACGACAACGCGGCGCTGGCCGTGTTCCCCGAACTGA
CGCTGACGGGTTATTCGATCGAGGACATCGTCATGCAGGACGGGTTGCTCGAGGCGGTCGAAGCCGCCCTGCTGCAGGTG
GTGACGGCGTCGGCGGAGCTGCTGCCGGTTCTCGTGGTGGGTGCGCCACTGCGTTACCGCCACCGGGTCTACAACACCGC
GGTGGTCATTCACCGCGGGCAGGTCCTCGGCGTGGTCCCGAAGTCCTACATCCCCAATTACCGTGAGTTCTACGAGAATC
GCCAGATCGCCGCCGGCGACGAGGAGCGCGGTGAGATCCGGGTCGGAGGGCAGGAGGTGCCGTTCGGTCCGGATCTGCTG
TTCGAGGCGACCGATGTCCCCGGTTTCGTGCTGCACGTCGAGATCTGCGAGGACATGTGGGTTCCGGTGCCCCCGAGCGC
CGAGGCGGCGCTGGCCGGGGCGACGGTGCTGGCCAACCTGTCCGGCAGCCCGATCACCATCGGCCGGTCCGAGGACCGGT
GCCTGTTGGCGCGTTCGGCGTCGTCGCGGTGTCTGGCCGCCTACGTCTACGCCGCCGCGGGAGAAGGGGAATCCACGACC
GATCTGGCCTGGGACGGGCAGACGATGATCTGGGAGAACGGGTCGTGCCTGGCGCAGTCCGAGCGATTCCCCAAGGGTGA
ACGCCGCTCGATCGCCGATGTCGACCTCCAGCTGCTCCGCAACGAACGACTGCGCATGGGTACTTTCGACGACAACCGCC
GCCACCATCTGATCGACGAGGACTCCTTCCGCCGCATCGAGTTCACGCTCGACCCGCCGACCGGCGACATCGGTCTGTAT
CGCGAGGTGGAGCGGTTCCCGTTCGTACCCGCGGATCCCGCCCGGCTGGAACAGGACTGCTACGAGGCGTACAACATTCA
GGTGGCCGGCCTCGAACAGCGGCTGCGCGCACTGCATTACCCCAAGATCGTGCTCGGGCTCTCCGGCGGCCTGGACTCCA
CACACGCGCTGATCGTCGCCGCGCGGGCGATGGACCGGGAGGAGCGGCCGCGCAGCGACATCCTCGCGTTCACGCTGCCG
GGTTTCGCGACCGGTGAGCACACCAGGAACAACGCCACCCGGCTGGCCGAGGCGCTCGGTGTGACATTCGAGACCATCGA
CATCACGTCGACGGCCAAGTTGATGCTCACGGAGATGGACCACCCGTTCTCGCGCGGCGAAAAGGTCTACGACGTCACGT
TCGAGAACGTGCAGGCCGGACTGCGCACGGATTACCTGTTCCGCCTTGCCAATCAGCGCGGTGGCATCGTGCTGGGCACC
GGCGACCTGTCCGAGCTGGCGCTGGGCTGGTCCACTTACGGTGTGGGCGACCAGATGTCGCACTACAACGTCAACGGGGG
TGTGCCGAAGACATTGATCCAGCACCTCATCCGATGGGTCATCCTCTCGAAGCAGTTCGACGACACCGTCAACGAGGTCC
TGCAGTCGGTGCTCGACACCGAGATCACCCCGGAACTGGTGCCCTCCGGTGAGGACGAGGAGATCCAGAGCAGTGAAGCC
AAGGTGGGACCCTATGTCCTGCAGGACTTCTCGCTGTTCCAGGTGCTGCGTTACGGGTTCCGGCCGTCGAAGGTCGCCTT
CCTGGCGTGGCACGCCTGGCGCGATGCCGACAGCGGCAACTGGCCCACCGGATTCCCGGACAGCAAGCGGCCCAGCTACA
CCCTCGACGAGATCAGGCACTGGCTGCAGGTGTTCGCCCAGCGGTTCTACTCGTTCAGCCAGTTCAAGCGCTCGGCGCTG
CCGAACGGGCCCAAGGTGTCCGCAGGGGGTTCCCTGTCCCCGCGCGGTGACTGGCGCGCGCCGTCGGACATGTCCGCGCG
AGTGTGGCTCGACGAGATCGAGAACGCGATCCCGAAGGGCTGA

Upstream 100 bases:

>100_bases
CGTCCGGATGGTCGTAGGTATTCATCATGTCGATGATCAGCACCGCGGTCTTGGTCATGTCGCTTGCATACCCCGCGACC
AATGCGGGCAACAATGTGGC

Downstream 100 bases:

>100_bases
CTATTCGGGTTCGTCGTCGTCTGCCTTGTCGGTGAGGTAGCGGTGGGGGTGGAAGTGGTTGTTGATGCGGGGTTGGCCGG
TGTCCTGTTCCTTGGGTGGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MDFYSAYRHGFARIAACTHHTSIADPKGNAESVLRMARDCHDDNAALAVFPELTLTGYSIEDIVMQDGLLEAVEAALLQV
VTASAELLPVLVVGAPLRYRHRVYNTAVVIHRGQVLGVVPKSYIPNYREFYENRQIAAGDEERGEIRVGGQEVPFGPDLL
FEATDVPGFVLHVEICEDMWVPVPPSAEAALAGATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGSCLAQSERFPKGERRSIADVDLQLLRNERLRMGTFDDNRRHHLIDEDSFRRIEFTLDPPTGDIGLY
REVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALHYPKIVLGLSGGLDSTHALIVAARAMDREERPRSDILAFTLP
GFATGEHTRNNATRLAEALGVTFETIDITSTAKLMLTEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVILSKQFDDTVNEVLQSVLDTEITPELVPSGEDEEIQSSEA
KVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWRDADSGNWPTGFPDSKRPSYTLDEIRHWLQVFAQRFYSFSQFKRSAL
PNGPKVSAGGSLSPRGDWRAPSDMSARVWLDEIENAIPKG

Sequences:

>Translated_680_residues
MDFYSAYRHGFARIAACTHHTSIADPKGNAESVLRMARDCHDDNAALAVFPELTLTGYSIEDIVMQDGLLEAVEAALLQV
VTASAELLPVLVVGAPLRYRHRVYNTAVVIHRGQVLGVVPKSYIPNYREFYENRQIAAGDEERGEIRVGGQEVPFGPDLL
FEATDVPGFVLHVEICEDMWVPVPPSAEAALAGATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGSCLAQSERFPKGERRSIADVDLQLLRNERLRMGTFDDNRRHHLIDEDSFRRIEFTLDPPTGDIGLY
REVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALHYPKIVLGLSGGLDSTHALIVAARAMDREERPRSDILAFTLP
GFATGEHTRNNATRLAEALGVTFETIDITSTAKLMLTEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVILSKQFDDTVNEVLQSVLDTEITPELVPSGEDEEIQSSEA
KVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWRDADSGNWPTGFPDSKRPSYTLDEIRHWLQVFAQRFYSFSQFKRSAL
PNGPKVSAGGSLSPRGDWRAPSDMSARVWLDEIENAIPKG
>Mature_680_residues
MDFYSAYRHGFARIAACTHHTSIADPKGNAESVLRMARDCHDDNAALAVFPELTLTGYSIEDIVMQDGLLEAVEAALLQV
VTASAELLPVLVVGAPLRYRHRVYNTAVVIHRGQVLGVVPKSYIPNYREFYENRQIAAGDEERGEIRVGGQEVPFGPDLL
FEATDVPGFVLHVEICEDMWVPVPPSAEAALAGATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGSCLAQSERFPKGERRSIADVDLQLLRNERLRMGTFDDNRRHHLIDEDSFRRIEFTLDPPTGDIGLY
REVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALHYPKIVLGLSGGLDSTHALIVAARAMDREERPRSDILAFTLP
GFATGEHTRNNATRLAEALGVTFETIDITSTAKLMLTEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVILSKQFDDTVNEVLQSVLDTEITPELVPSGEDEEIQSSEA
KVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWRDADSGNWPTGFPDSKRPSYTLDEIRHWLQVFAQRFYSFSQFKRSAL
PNGPKVSAGGSLSPRGDWRAPSDMSARVWLDEIENAIPKG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=624, Percent_Identity=23.5576923076923, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538756, Length=696, Percent_Identity=24.2816091954023, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=649, Percent_Identity=24.8073959938367, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24641841, Length=680, Percent_Identity=23.9705882352941, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24641843, Length=680, Percent_Identity=23.9705882352941, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 75572; Mature: 75572

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFYSAYRHGFARIAACTHHTSIADPKGNAESVLRMARDCHDDNAALAVFPELTLTGYSI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEEECCCCH
EDIVMQDGLLEAVEAALLQVVTASAELLPVLVVGAPLRYRHRVYNTAVVIHRGQVLGVVP
HHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHEEEEEEECCCEEEECC
KSYIPNYREFYENRQIAAGDEERGEIRVGGQEVPFGPDLLFEATDVPGFVLHVEICEDMW
HHHCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEEEEECCCCC
VPVPPSAEAALAGATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCCCCEEEECCCCCCEEEEEEEEECCCCCCCC
DLAWDGQTMIWENGSCLAQSERFPKGERRSIADVDLQLLRNERLRMGTFDDNRRHHLIDE
EEEECCCEEEEECCCEEEHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
DSFRRIEFTLDPPTGDIGLYREVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALHY
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHHCC
PKIVLGLSGGLDSTHALIVAARAMDREERPRSDILAFTLPGFATGEHTRNNATRLAEALG
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
VTFETIDITSTAKLMLTEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT
CEEEEEECCCCHHEEEECCCCCHHCCCEEEEEEHHHHHHCCHHHHHHHHHCCCCCEEEEC
GDLSELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVILSKQFDDTVNEVLQSVLDT
CCHHHHHHCCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EITPELVPSGEDEEIQSSEAKVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWRDADSGN
CCCHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
WPTGFPDSKRPSYTLDEIRHWLQVFAQRFYSFSQFKRSALPNGPKVSAGGSLSPRGDWRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSDMSARVWLDEIENAIPKG
CCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFYSAYRHGFARIAACTHHTSIADPKGNAESVLRMARDCHDDNAALAVFPELTLTGYSI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEEECCCCH
EDIVMQDGLLEAVEAALLQVVTASAELLPVLVVGAPLRYRHRVYNTAVVIHRGQVLGVVP
HHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHEEEEEEECCCEEEECC
KSYIPNYREFYENRQIAAGDEERGEIRVGGQEVPFGPDLLFEATDVPGFVLHVEICEDMW
HHHCCCHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEEEEECCCCC
VPVPPSAEAALAGATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCCCCEEEECCCCCCEEEEEEEEECCCCCCCC
DLAWDGQTMIWENGSCLAQSERFPKGERRSIADVDLQLLRNERLRMGTFDDNRRHHLIDE
EEEECCCEEEEECCCEEEHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
DSFRRIEFTLDPPTGDIGLYREVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALHY
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHHCC
PKIVLGLSGGLDSTHALIVAARAMDREERPRSDILAFTLPGFATGEHTRNNATRLAEALG
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
VTFETIDITSTAKLMLTEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGT
CEEEEEECCCCHHEEEECCCCCHHCCCEEEEEEHHHHHHCCHHHHHHHHHCCCCCEEEEC
GDLSELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVILSKQFDDTVNEVLQSVLDT
CCHHHHHHCCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EITPELVPSGEDEEIQSSEAKVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWRDADSGN
CCCHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
WPTGFPDSKRPSYTLDEIRHWLQVFAQRFYSFSQFKRSALPNGPKVSAGGSLSPRGDWRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PSDMSARVWLDEIENAIPKG
CCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]