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The map label for this gene is pinE [C]

Identifier: 108800484

GI number: 108800484

Start: 3745942

End: 3747324

Strand: Reverse

Name: pinE [C]

Synonym: Mmcs_3518

Alternate gene names: 108800484

Gene position: 3747324-3745942 (Counterclockwise)

Preceding gene: 108800485

Following gene: 108800483

Centisome position: 65.68

GC content: 66.81

Gene sequence:

>1383_bases
ATGGATAGACTCGTCGGCATCTACTGCCGAATCAGCGCCGACGCGAGTGGCGACGCGCTCGGTGTCCAGCGGCAAGAGGC
CGACGCGCGGAAGCTGTGCGCTGAGCGCGGTTGGACCGTCGTAGAGGTGTTCTGCGACAACGACCGGTCAGCGTATGACC
GACTCAAGCAGCGGCCCCGCTACCTCGCGATGCTCGACGCCATCAGGGCTGGCGAGATTAACGGCATCGTCGCGTGGCAC
CCGGACCGATTGCACCGGCAGACACGCGAGCTCGTGCCGTTCATCGACCTGGTCAATGAGTTCGGCGTGACGGTCGAGAC
TGTGACAGCGGGGGCGTACGACCTCACAACGCCGTCAGGGCGCATGAATGCCCGCATCGTCGGGTCGGTTGCCGAGTTCG
AATCTGAGCACAAGTCAGAGCGCATTCGGCGCAAGCTTGAGGCCAACGCCGCCGAGGGGCGCCACCATGGCGGGTCGCGG
CCGTACGGGTGGCTGGACGACCGCGTGAGCATCCATCCGACCGAGGCCGCGGTTGTGCGTGAGGCTGCTGCGCGAGTGCT
GGCGGGCGAGTCCGTCAAGAGCATTGCACGCGCGCTGAACGCTGCGGGGCACACGACCTCAACGGGCAGACCGTGGCGCG
ACGTGAACGTCCGAGACATGTTGGTCAGGCCGCGCAACGCTGGCTTGCGGGTCCATCACGGCGAGGTGGTCGGCGGCGGC
AGGTGGGAGGCAATTCTGCCGGCCGACGATCACCACCAGGTGGTCGCGATCCTGACCGACCCCGCGCGACGTACGACCCC
CGGCCGCGACGGACGTATCCACCTGCTCTCCGCGCTGGCGCGCTGCGGTGTCTGCGACGAGCCGGTGAGGGTGGGGAGAA
GCAAGCCGTACAAAGGCGTGTCGAAGCCGATTTACCGTTGTATGGCGGCGCACGTGATCCGCGATCAGGCGTCGGTCGAC
GACTTGGTTACCCACGTGGTCATCGGTCGGCTGGCAATGGACGACGCCGCCGCATTGCTCGTCGAACCCGAACGTAAAGA
CGACGCGCACGCCGCCGCGGGGCGCATCGACGAGATACAGCAGCGGCTCGGCGCTGCTGCGTCTGCGTTCGCCGCCGGAG
CGATCACGCTGCCCCAATTGACCATCATCACCGCTGAGCTTCGGCCCCAGCTCGACGAGGCGCAGACGGCGGCTGCGTCG
CCGAGCAGAGAGAAGGTGCTCGGTGATCTGGTCAAGGCGAAGGACCCCGCGGCGGTGTGGGAAGCGATGACCCCGCAGCG
CCGCCGTACCGTCATCGACCTGCTCGTCGCTGTTCACATCATGCCGACGTCGAAGGGGCCGCGATTCAACCCTGACGCGG
TGGATATTCAGTGGAAGACCTAG

Upstream 100 bases:

>100_bases
CTGCCCGCTCTCGTACTCGGTGACACCGCGACCGTTCCGCCGGACACGACGGCGGACTTCCGTACCGCGGGTTTGACCCA
TCTGAGCCTATCATTGAGGC

Downstream 100 bases:

>100_bases
CCGGTGTCAATACCTATGACACACAAGGGAATTCGGCTGCTCTCTGGTATAGTCATCAGAAATCCGGCAATGAGGTCTAG
TCGGCGGGACCGTTCGAGAC

Product: recombinase

Products: NA

Alternate protein names: Resolvase Domain-Containing Protein; Resolvase Domain Protein; Site-Specific Recombinase DNA Invertase Pin; Integrase/Recombinase; Site-Specific Recombinase; Resolvase Domain; Resolvase; Site-Specific Recombinase For Integration And Excision; Integrase; DNA Recombinase; Site-Specific DNA Recombinase; Prophage Protein; Transposase; Cassette Chromosome Recombinase B; Resolvase-Like Protein; Integrase /Recombinase; Ntegrase/Recombinase.; Site-Specific Integrase; Site-Specific RecombinaIVCA; Resolvase/Recombinase Domain Protein; PhiRv1 Phage Integrase

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDRLVGIYCRISADASGDALGVQRQEADARKLCAERGWTVVEVFCDNDRSAYDRLKQRPRYLAMLDAIRAGEINGIVAWH
PDRLHRQTRELVPFIDLVNEFGVTVETVTAGAYDLTTPSGRMNARIVGSVAEFESEHKSERIRRKLEANAAEGRHHGGSR
PYGWLDDRVSIHPTEAAVVREAAARVLAGESVKSIARALNAAGHTTSTGRPWRDVNVRDMLVRPRNAGLRVHHGEVVGGG
RWEAILPADDHHQVVAILTDPARRTTPGRDGRIHLLSALARCGVCDEPVRVGRSKPYKGVSKPIYRCMAAHVIRDQASVD
DLVTHVVIGRLAMDDAAALLVEPERKDDAHAAAGRIDEIQQRLGAAASAFAAGAITLPQLTIITAELRPQLDEAQTAAAS
PSREKVLGDLVKAKDPAAVWEAMTPQRRRTVIDLLVAVHIMPTSKGPRFNPDAVDIQWKT

Sequences:

>Translated_460_residues
MDRLVGIYCRISADASGDALGVQRQEADARKLCAERGWTVVEVFCDNDRSAYDRLKQRPRYLAMLDAIRAGEINGIVAWH
PDRLHRQTRELVPFIDLVNEFGVTVETVTAGAYDLTTPSGRMNARIVGSVAEFESEHKSERIRRKLEANAAEGRHHGGSR
PYGWLDDRVSIHPTEAAVVREAAARVLAGESVKSIARALNAAGHTTSTGRPWRDVNVRDMLVRPRNAGLRVHHGEVVGGG
RWEAILPADDHHQVVAILTDPARRTTPGRDGRIHLLSALARCGVCDEPVRVGRSKPYKGVSKPIYRCMAAHVIRDQASVD
DLVTHVVIGRLAMDDAAALLVEPERKDDAHAAAGRIDEIQQRLGAAASAFAAGAITLPQLTIITAELRPQLDEAQTAAAS
PSREKVLGDLVKAKDPAAVWEAMTPQRRRTVIDLLVAVHIMPTSKGPRFNPDAVDIQWKT
>Mature_460_residues
MDRLVGIYCRISADASGDALGVQRQEADARKLCAERGWTVVEVFCDNDRSAYDRLKQRPRYLAMLDAIRAGEINGIVAWH
PDRLHRQTRELVPFIDLVNEFGVTVETVTAGAYDLTTPSGRMNARIVGSVAEFESEHKSERIRRKLEANAAEGRHHGGSR
PYGWLDDRVSIHPTEAAVVREAAARVLAGESVKSIARALNAAGHTTSTGRPWRDVNVRDMLVRPRNAGLRVHHGEVVGGG
RWEAILPADDHHQVVAILTDPARRTTPGRDGRIHLLSALARCGVCDEPVRVGRSKPYKGVSKPIYRCMAAHVIRDQASVD
DLVTHVVIGRLAMDDAAALLVEPERKDDAHAAAGRIDEIQQRLGAAASAFAAGAITLPQLTIITAELRPQLDEAQTAAAS
PSREKVLGDLVKAKDPAAVWEAMTPQRRRTVIDLLVAVHIMPTSKGPRFNPDAVDIQWKT

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50335; Mature: 50335

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLVGIYCRISADASGDALGVQRQEADARKLCAERGWTVVEVFCDNDRSAYDRLKQRPR
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCH
YLAMLDAIRAGEINGIVAWHPDRLHRQTRELVPFIDLVNEFGVTVETVTAGAYDLTTPSG
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECCCCCC
RMNARIVGSVAEFESEHKSERIRRKLEANAAEGRHHGGSRPYGWLDDRVSIHPTEAAVVR
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHH
EAAARVLAGESVKSIARALNAAGHTTSTGRPWRDVNVRDMLVRPRNAGLRVHHGEVVGGG
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCEEEECCEEECCC
RWEAILPADDHHQVVAILTDPARRTTPGRDGRIHLLSALARCGVCDEPVRVGRSKPYKGV
CEEEECCCCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCCCH
SKPIYRCMAAHVIRDQASVDDLVTHVVIGRLAMDDAAALLVEPERKDDAHAAAGRIDEIQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCHHHHH
QRLGAAASAFAAGAITLPQLTIITAELRPQLDEAQTAAASPSREKVLGDLVKAKDPAAVW
HHHHHHHHHHHHCCEECCEEEEEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
EAMTPQRRRTVIDLLVAVHIMPTSKGPRFNPDAVDIQWKT
HHCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MDRLVGIYCRISADASGDALGVQRQEADARKLCAERGWTVVEVFCDNDRSAYDRLKQRPR
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCH
YLAMLDAIRAGEINGIVAWHPDRLHRQTRELVPFIDLVNEFGVTVETVTAGAYDLTTPSG
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECCCCCC
RMNARIVGSVAEFESEHKSERIRRKLEANAAEGRHHGGSRPYGWLDDRVSIHPTEAAVVR
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHH
EAAARVLAGESVKSIARALNAAGHTTSTGRPWRDVNVRDMLVRPRNAGLRVHHGEVVGGG
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCEEEECCEEECCC
RWEAILPADDHHQVVAILTDPARRTTPGRDGRIHLLSALARCGVCDEPVRVGRSKPYKGV
CEEEECCCCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCCCCCH
SKPIYRCMAAHVIRDQASVDDLVTHVVIGRLAMDDAAALLVEPERKDDAHAAAGRIDEIQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCHHHHH
QRLGAAASAFAAGAITLPQLTIITAELRPQLDEAQTAAASPSREKVLGDLVKAKDPAAVW
HHHHHHHHHHHHCCEECCEEEEEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
EAMTPQRRRTVIDLLVAVHIMPTSKGPRFNPDAVDIQWKT
HHCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA