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The map label for this gene is dnaG [H]

Identifier: 108800408

GI number: 108800408

Start: 3651928

End: 3653850

Strand: Reverse

Name: dnaG [H]

Synonym: Mmcs_3442

Alternate gene names: 108800408

Gene position: 3653850-3651928 (Counterclockwise)

Preceding gene: 108800409

Following gene: 108800407

Centisome position: 64.04

GC content: 69.94

Gene sequence:

>1923_bases
GTGGCCGGGCGGATACCAGATCGCGACATCGCGGCCATTCGTGAACGCGTGCGCATCGAGGATGTCGTCGGCGACTACGT
GCAGTTGCGCCGCGCCGGCGCCGATTCGCTCAAGGGCCTGTGCCCGTTCCACGACGAGAAGTCGCCGTCGTTCCACGTCC
GGCCCAACCACGGCCACTTCCACTGCTTCGGCTGCGGTGAGGGCGGTGACGTCTACGCGTTCGTCCAGAAGATCGAGCAC
GTCAGCTTCGTCGAATCGGTGGAACTGCTCGCCGACCGCATCGGCTACACCGTCACCTACACCGGCGCCTCGAACACCAA
CGTGCAGCGCGACCGGGGCAGCCGCAGCCGGCTGCTGGCGGCCAACGCCGCCGCGCAGGAGTTCTACGCCGAGGCGCTGA
CATCCGACGAGGCCGCCCCGGCCCGGCAGTACCTCATCGAGCGCAACTTCGACGCCCAGGCGGCCGCCCGCTTCGGGTGC
GGTTTCGCCCCCTCTGGCTGGGACAAGCTCACCAAACACTTGCTGCGCAAGGGATTCGAGTTCAAGGAGCTCGAGGCGGC
CGGACTGGCCCGGGAGGGCAAACGCGGTCCGATGGACCGCTTCCACCGCCGGTTGCTCTGGCCGATCCGGGCGGCCGGGG
GAGAGGTGATCGGATTCGGCGCGCGGCGGATCTTCGACGACGATCAGATGGAAGCCAAGTACGTCAACACCCCCGAGACC
GTCCTGTACAAGAAGTCGGCGGTGCTCTTCGGCCTGGACCTGGCCAAACGCGATATCGCCAAATCCCACCGGGCCGTCGT
CGTCGAGGGGTACACCGACGTGATGGCCATGCACCTGGCCAACGAGACCACCGCCGTCGCCTCGTGCGGAACGGCCTTCG
GCGAAGGCCACCTCGCGCTGCTGCGCCGGTTGATGATGGACGACAACTGGTACCGCGGCGAGCTGATCTTCGTGTTCGAC
GGGGACGCCGCCGGACAGGCCGCCGCGCTCAAGGCGTTCGACGGTGATCAGCAGGTGGCCGGCAAGTCGTTCGTCGCGGT
CGCCGCCGACGGGATGGATCCCTGCGATCTGCGCCTGAAGTCAGGGGACAACGCGCTGCGCGATCTGGTGGCGCGGCGGA
TCCCGATGTTCGAGTTCGCCATCCGCAGCCTGATCCCCGCCGGCGACGTCCTCGACAACGATCCCGCCGCGCAGGTGGAG
GCGCTGCGCCGCTGCGTACCCCTGGTGTCCCGGATCCGTGACTACGCGCTGCGCGACGAGTACGCGCGCCGGCTGGCCGG
CTGGACCGGATGGGCCGACGAGGCGCAGGTGCTCAACCGGGTGCGCGAAGAGGCGGCCAAGCGCGGGATGCCGGACCGCG
GCCGCCGGCGCAACGGAGCGGATGCGGCGCCCGCCCAACCGCGGCGCGCGCCGGATGTGGCCGCGCGGCCCGATCCGGCC
GATCCGACGCTGTGGCCGCAGCGCGAGGCGCTCAAATCGGCGCTGCAGTATCCCGCGCTGGCCGGTCCGGTGTTCGACGC
GCTGGCCGTCGACAGCTTCACCCACCCCGGATACCGCGCGGTGCGCACCGCGATCGAGGCGGCGGGCGGTGCCGCCGCCG
GGCTCTCGGGTGCGCAGTGGATCGAGGTGGTGCGTGAGCAGACCGCGAGCCCGCAGGCGGCCAACCTGGTCAACGAGTTG
AGCGTCGAGGCCATCCGGGTCGAGGACGACGACAAGCTGCCCCGCTACATCGGCGGAGTCCTGGCCCGTCTCCAGGAGGT
CTGGGTGGGCCGGCAGATCGCCGAGGTGAAGTCGAAACTGCAGCGCATGTCGCCGGTGGAGCAGGGCGACGAGTACCACG
CGCTGTTCGGCGACCTGGTGGCTATGGAGGCCTACCGCCGCAGCCTGCTCGAACAGGCCAGCGGGGATGACTTGACTGCG
TGA

Upstream 100 bases:

>100_bases
GCCCGGCTGCGCGTGGTGATCGATCAGATCGCGTCCTACACCGAGGGGCGGTTGGAACGAGTGCACGAAGCCCGGTCGCC
CCGACCTCTAGACTGACCCG

Downstream 100 bases:

>100_bases
TACCGCGTAACACGATATGGTGAGCGCGCTATAACCCGTTGGGGGAACGGCGAGGGAAGGCAACACCTGTGGCAACCATG
ATTCGGATCCGGCGCTCGGT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MAGRIPDRDIAAIRERVRIEDVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKIEH
VSFVESVELLADRIGYTVTYTGASNTNVQRDRGSRSRLLAANAAAQEFYAEALTSDEAAPARQYLIERNFDAQAAARFGC
GFAPSGWDKLTKHLLRKGFEFKELEAAGLAREGKRGPMDRFHRRLLWPIRAAGGEVIGFGARRIFDDDQMEAKYVNTPET
VLYKKSAVLFGLDLAKRDIAKSHRAVVVEGYTDVMAMHLANETTAVASCGTAFGEGHLALLRRLMMDDNWYRGELIFVFD
GDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLKSGDNALRDLVARRIPMFEFAIRSLIPAGDVLDNDPAAQVE
ALRRCVPLVSRIRDYALRDEYARRLAGWTGWADEAQVLNRVREEAAKRGMPDRGRRRNGADAAPAQPRRAPDVAARPDPA
DPTLWPQREALKSALQYPALAGPVFDALAVDSFTHPGYRAVRTAIEAAGGAAAGLSGAQWIEVVREQTASPQAANLVNEL
SVEAIRVEDDDKLPRYIGGVLARLQEVWVGRQIAEVKSKLQRMSPVEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA

Sequences:

>Translated_640_residues
MAGRIPDRDIAAIRERVRIEDVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKIEH
VSFVESVELLADRIGYTVTYTGASNTNVQRDRGSRSRLLAANAAAQEFYAEALTSDEAAPARQYLIERNFDAQAAARFGC
GFAPSGWDKLTKHLLRKGFEFKELEAAGLAREGKRGPMDRFHRRLLWPIRAAGGEVIGFGARRIFDDDQMEAKYVNTPET
VLYKKSAVLFGLDLAKRDIAKSHRAVVVEGYTDVMAMHLANETTAVASCGTAFGEGHLALLRRLMMDDNWYRGELIFVFD
GDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLKSGDNALRDLVARRIPMFEFAIRSLIPAGDVLDNDPAAQVE
ALRRCVPLVSRIRDYALRDEYARRLAGWTGWADEAQVLNRVREEAAKRGMPDRGRRRNGADAAPAQPRRAPDVAARPDPA
DPTLWPQREALKSALQYPALAGPVFDALAVDSFTHPGYRAVRTAIEAAGGAAAGLSGAQWIEVVREQTASPQAANLVNEL
SVEAIRVEDDDKLPRYIGGVLARLQEVWVGRQIAEVKSKLQRMSPVEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
>Mature_639_residues
AGRIPDRDIAAIRERVRIEDVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKIEHV
SFVESVELLADRIGYTVTYTGASNTNVQRDRGSRSRLLAANAAAQEFYAEALTSDEAAPARQYLIERNFDAQAAARFGCG
FAPSGWDKLTKHLLRKGFEFKELEAAGLAREGKRGPMDRFHRRLLWPIRAAGGEVIGFGARRIFDDDQMEAKYVNTPETV
LYKKSAVLFGLDLAKRDIAKSHRAVVVEGYTDVMAMHLANETTAVASCGTAFGEGHLALLRRLMMDDNWYRGELIFVFDG
DAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLKSGDNALRDLVARRIPMFEFAIRSLIPAGDVLDNDPAAQVEA
LRRCVPLVSRIRDYALRDEYARRLAGWTGWADEAQVLNRVREEAAKRGMPDRGRRRNGADAAPAQPRRAPDVAARPDPAD
PTLWPQREALKSALQYPALAGPVFDALAVDSFTHPGYRAVRTAIEAAGGAAAGLSGAQWIEVVREQTASPQAANLVNELS
VEAIRVEDDDKLPRYIGGVLARLQEVWVGRQIAEVKSKLQRMSPVEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=514, Percent_Identity=32.8793774319066, Blast_Score=231, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70293; Mature: 70162

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPDRDIAAIRERVRIEDVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCGEGGDVYAFVQKIEHVSFVESVELLADRIGYTVTYTGASNTNVQRDRGSRSRLLA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
ANAAAQEFYAEALTSDEAAPARQYLIERNFDAQAAARFGCGFAPSGWDKLTKHLLRKGFE
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
FKELEAAGLAREGKRGPMDRFHRRLLWPIRAAGGEVIGFGARRIFDDDQMEAKYVNTPET
HHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCEECCCCHH
VLYKKSAVLFGLDLAKRDIAKSHRAVVVEGYTDVMAMHLANETTAVASCGTAFGEGHLAL
HHEECCHHEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHCHHCCCCHHHH
LRRLMMDDNWYRGELIFVFDGDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLK
HHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEEEECCCCCCCEEEC
SGDNALRDLVARRIPMFEFAIRSLIPAGDVLDNDPAAQVEALRRCVPLVSRIRDYALRDE
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YARRLAGWTGWADEAQVLNRVREEAAKRGMPDRGRRRNGADAAPAQPRRAPDVAARPDPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DPTLWPQREALKSALQYPALAGPVFDALAVDSFTHPGYRAVRTAIEAAGGAAAGLSGAQW
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
IEVVREQTASPQAANLVNELSVEAIRVEDDDKLPRYIGGVLARLQEVWVGRQIAEVKSKL
HHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMSPVEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
HHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGRIPDRDIAAIRERVRIEDVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCGEGGDVYAFVQKIEHVSFVESVELLADRIGYTVTYTGASNTNVQRDRGSRSRLLA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
ANAAAQEFYAEALTSDEAAPARQYLIERNFDAQAAARFGCGFAPSGWDKLTKHLLRKGFE
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
FKELEAAGLAREGKRGPMDRFHRRLLWPIRAAGGEVIGFGARRIFDDDQMEAKYVNTPET
HHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCEECCCCHH
VLYKKSAVLFGLDLAKRDIAKSHRAVVVEGYTDVMAMHLANETTAVASCGTAFGEGHLAL
HHEECCHHEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHCHHCCCCHHHH
LRRLMMDDNWYRGELIFVFDGDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLK
HHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEEEECCCCCCCEEEC
SGDNALRDLVARRIPMFEFAIRSLIPAGDVLDNDPAAQVEALRRCVPLVSRIRDYALRDE
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YARRLAGWTGWADEAQVLNRVREEAAKRGMPDRGRRRNGADAAPAQPRRAPDVAARPDPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DPTLWPQREALKSALQYPALAGPVFDALAVDSFTHPGYRAVRTAIEAAGGAAAGLSGAQW
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
IEVVREQTASPQAANLVNELSVEAIRVEDDDKLPRYIGGVLARLQEVWVGRQIAEVKSKL
HHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMSPVEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
HHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]