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The map label for this gene is caiD [H]

Identifier: 108800174

GI number: 108800174

Start: 3402557

End: 3403324

Strand: Reverse

Name: caiD [H]

Synonym: Mmcs_3208

Alternate gene names: 108800174

Gene position: 3403324-3402557 (Counterclockwise)

Preceding gene: 108800175

Following gene: 108800173

Centisome position: 59.65

GC content: 68.23

Gene sequence:

>768_bases
GTGGCCGAGCAACCGGTTCGCTACGAGGTCGTCGACAGCGTGGCCTGGCTGACGATCAACCGGCCCGAGGCGCGCAACGC
GCTGAACAACGCTGTGCGCACGGGGCTTTTCGACGCGGTACGCCGCTTCAATGACGACGACGCGGCGAAGGTGCTCGTCC
TCACCGGCGTGGGCGATAAGGCGTTCTGCGCCGGCGGGGACTTGAAGGAGATGGCGCAGAACGCGCTCAAGGTGCCCCCG
AAGGACTTCGCTCCGCAGTTCGGCCGCAACATCGATGTCGCGAAGCCGACGATCGCGGCCGTCAACGGTGTCGCGTTCGC
CGGGGGCTTCCTGCTCGCGCAGCAGTGCGACCTGGTCGTGGCTGCCGAACACGCGACCTTCGCCGTCAGCGAGGTCAAAG
TCGGCCGCGGGTCGCCGTGGGCGGCACCGCTATCGTGGCTGGTGCCCCCACGCGTTGCCATGCAGATCCTGCTGACCGGC
GACCCGATCACCGCCGAGCGCGCCCACCAGGTCGGCTTAGTCAATGAGGTGGTGCCGGCCGATCAGTTGCGCGAGCGTAC
CCGGCAGTTGGCGCTCAGCATCGCCGCGAACGCACCGCTATCGGTGCTTGCGTCCAAGCGCACCGTGTACCTCTCGGCAC
AGCACCACCTCGCCGCCGCCTACGACCTGGCCGACGAGATATGGGAGCCGGTCTATCTGAGCGACGACGCGCAGGAAGGC
CCGACGGCGTTCCGCGAGAAGCGCGCACCACAGTGGAAGGGACGCTGA

Upstream 100 bases:

>100_bases
GTGCCGAGTGAAGCCACCGTTGAGCTGGCCGACCCGTTTGTCGAGGTGCCGGGGTGAGCTGCCACTCACATGCGAGGCGA
CGGTGAGGGACGGAGGCTCG

Downstream 100 bases:

>100_bases
CATGGCGGTGACCATGCAGTCGGTAATCGCCGACATCGAAGCCGAAACGGCCGCGTTGCGCGAACTCATCGCACCGCTGC
CCGAAGGTCCGCGCGGCTGG

Product: enoyl-CoA hydratase/isomerase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAEQPVRYEVVDSVAWLTINRPEARNALNNAVRTGLFDAVRRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPP
KDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWAAPLSWLVPPRVAMQILLTG
DPITAERAHQVGLVNEVVPADQLRERTRQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
PTAFREKRAPQWKGR

Sequences:

>Translated_255_residues
MAEQPVRYEVVDSVAWLTINRPEARNALNNAVRTGLFDAVRRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPP
KDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWAAPLSWLVPPRVAMQILLTG
DPITAERAHQVGLVNEVVPADQLRERTRQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
PTAFREKRAPQWKGR
>Mature_254_residues
AEQPVRYEVVDSVAWLTINRPEARNALNNAVRTGLFDAVRRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPPK
DFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWAAPLSWLVPPRVAMQILLTGD
PITAERAHQVGLVNEVVPADQLRERTRQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEGP
TAFREKRAPQWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=37.9591836734694, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=31.6602316602317, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI70995211, Length=233, Percent_Identity=29.1845493562232, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=25.4980079681275, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=25.4980079681275, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=27.843137254902, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=26.4248704663212, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=26.4248704663212, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=38.7755102040816, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=36.3636363636364, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=32.3076923076923, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=28.8973384030418, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=32.5757575757576, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=37.3493975903614, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=35.3413654618474, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17560910, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=31.0756972111554, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=25.7777777777778, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=26.8482490272374, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=26.7543859649123, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=248, Percent_Identity=26.6129032258064, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=207, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=34.9794238683128, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=34.9794238683128, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=28.4584980237154, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=29.7169811320755, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24650670, Length=261, Percent_Identity=27.9693486590038, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=175, Percent_Identity=26.8571428571429, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583165, Length=174, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27585; Mature: 27453

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPVRYEVVDSVAWLTINRPEARNALNNAVRTGLFDAVRRFNDDDAAKVLVLTGVGDK
CCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGGDLKEMAQNALKVPPKDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVV
CEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEE
AAEHATFAVSEVKVGRGSPWAAPLSWLVPPRVAMQILLTGDPITAERAHQVGLVNEVVPA
EECCCEEEEEEEECCCCCCCCCCHHHCCCHHHHEEEEECCCCCCHHHHHHHCHHHHCCCH
DQLRERTRQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
HHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC
PTAFREKRAPQWKGR
CHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEQPVRYEVVDSVAWLTINRPEARNALNNAVRTGLFDAVRRFNDDDAAKVLVLTGVGDK
CCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGGDLKEMAQNALKVPPKDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVV
CEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEE
AAEHATFAVSEVKVGRGSPWAAPLSWLVPPRVAMQILLTGDPITAERAHQVGLVNEVVPA
EECCCEEEEEEEECCCCCCCCCCHHHCCCHHHHEEEEECCCCCCHHHHHHHCHHHHCCCH
DQLRERTRQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
HHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC
PTAFREKRAPQWKGR
CHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA