Click here to switch to the map view.

The map label for this gene is mshC

Identifier: 108800119

GI number: 108800119

Start: 3345881

End: 3347128

Strand: Reverse

Name: mshC

Synonym: Mmcs_3153

Alternate gene names: 108800119

Gene position: 3347128-3345881 (Counterclockwise)

Preceding gene: 108800120

Following gene: 108800118

Centisome position: 58.67

GC content: 70.35

Gene sequence:

>1248_bases
ATGGAATCGTGGTCCGCGCCGGAGGTTCCGGCCCTGCCGGGCCGGGGCCCGCAACTGCGGCTCTACGACAGCGCCGACCG
CCAGGTCCGGCCGGTCTCGGCGGGCGACACGGCCACCATGTACGTCTGCGGGATCACCCCGTACGACGCCACCCACCTCG
GCCACGCGGCCACCTATCTGGCGTTCGACCTCGTGCACCGGCTGTGGTTGGACGCCGGTCACCGGGTGCACTACGTGCAG
AACATCACCGACGTCGACGATCCGCTGTTCGAACGCGCTGCCCGCGACGGGATCGACTGGCGAGACCTGGGCGCGCGCGA
GATCCAGCTCTTCCGCGAGGACATGGCCGCGTTGCGGGTGTTGCCGCCGCACGACTACGTCGCGGCCACCGATGCGATCG
CCGAGGTGATCGAGTTGGTGGAGAAGATGCTGGCCTCCGGGGCGGCATACGTCGTCGACGACCCCGAGTTCCCCGACGTG
TACTACCGCGCGGACGCGACGGTCCAGTTCGGATACGAGTCGAACTACGACCACGAGACGATGCTCACACTGTTCGCCGA
ACGCGGCGGCGATCCGGACCGTGCCGGGAAGGCCGACGAACTCGACGCGCTGCTGTGGCGGGCGGAGCGCCCCGGCGAGC
CGAGCTGGCCGTCGCCGTTCGGTCCCGGGCGTCCCGGCTGGCACGTGGAATGCGCCGCCATCGCGCTCAGCCGCATCGGG
ACCGGCCTGGACATCCAGGGCGGCGGCAGCGACCTGATCTTCCCGCACCACGAGTTCTCCGCCGCCCACGCCGAATCGGT
CACGGGGGAGCGGCGTTTCGCGCGGCACTACGTGCACGCCGGAATGATCGGCTGGGACGGGCACAAGATGAGCAAGAGCC
GCGGCAACCTGGTGTTGGTGTCGCGGTTGCGCGCCGAGGGTGTCGACCCGTCGGCGATCCGCCTCGGGCTGCTGGCCGGT
CACTATCGCGAGGACCGGTTCTGGAGCGACGACGTGCTGTCCGACGCGCAGACCCGGCTGCAGCGCTGGCGTCGCGCCAC
CTCGCTGCCGACCGGACCCGATGCGACGGACGTGCTCGCGAGGGTGCGCACGTATCTCGCCGACGATCTGGACACCCCGA
AAGCGCTCATAGCGCTGGACGCCTGGTGTACCGAGGCGCTCGACGGTGGCGGCAGCGACGTGACAGCGCCCAAAACCGTG
GCCACGGCCGTCGACGCGTTGCTCGGAGTTGCGCTCCCCGTGGAGTAG

Upstream 100 bases:

>100_bases
CGTACTGACGGGGAATGAAAGTGCTGCCGTCGTTGTCGCACTGCGCGTGACTGCCCACCAGCGGGAGGCTGCCCTGATCG
AACAACCTAGAGTCGACGTC

Downstream 100 bases:

>100_bases
CTTGCGACGCATGACTTCCGTACACGGCAAGGTCGTGTTGATCACCGGTGGGGCCCGAGGGGTCGGCGAAGAACTGGCCC
GCCGGTTACATGCCAAGGGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MESWSAPEVPALPGRGPQLRLYDSADRQVRPVSAGDTATMYVCGITPYDATHLGHAATYLAFDLVHRLWLDAGHRVHYVQ
NITDVDDPLFERAARDGIDWRDLGAREIQLFREDMAALRVLPPHDYVAATDAIAEVIELVEKMLASGAAYVVDDPEFPDV
YYRADATVQFGYESNYDHETMLTLFAERGGDPDRAGKADELDALLWRAERPGEPSWPSPFGPGRPGWHVECAAIALSRIG
TGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSRLRAEGVDPSAIRLGLLAG
HYREDRFWSDDVLSDAQTRLQRWRRATSLPTGPDATDVLARVRTYLADDLDTPKALIALDAWCTEALDGGGSDVTAPKTV
ATAVDALLGVALPVE

Sequences:

>Translated_415_residues
MESWSAPEVPALPGRGPQLRLYDSADRQVRPVSAGDTATMYVCGITPYDATHLGHAATYLAFDLVHRLWLDAGHRVHYVQ
NITDVDDPLFERAARDGIDWRDLGAREIQLFREDMAALRVLPPHDYVAATDAIAEVIELVEKMLASGAAYVVDDPEFPDV
YYRADATVQFGYESNYDHETMLTLFAERGGDPDRAGKADELDALLWRAERPGEPSWPSPFGPGRPGWHVECAAIALSRIG
TGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSRLRAEGVDPSAIRLGLLAG
HYREDRFWSDDVLSDAQTRLQRWRRATSLPTGPDATDVLARVRTYLADDLDTPKALIALDAWCTEALDGGGSDVTAPKTV
ATAVDALLGVALPVE
>Mature_415_residues
MESWSAPEVPALPGRGPQLRLYDSADRQVRPVSAGDTATMYVCGITPYDATHLGHAATYLAFDLVHRLWLDAGHRVHYVQ
NITDVDDPLFERAARDGIDWRDLGAREIQLFREDMAALRVLPPHDYVAATDAIAEVIELVEKMLASGAAYVVDDPEFPDV
YYRADATVQFGYESNYDHETMLTLFAERGGDPDRAGKADELDALLWRAERPGEPSWPSPFGPGRPGWHVECAAIALSRIG
TGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARHYVHAGMIGWDGHKMSKSRGNLVLVSRLRAEGVDPSAIRLGLLAG
HYREDRFWSDDVLSDAQTRLQRWRRATSLPTGPDATDVLARVRTYLADDLDTPKALIALDAWCTEALDGGGSDVTAPKTV
ATAVDALLGVALPVE

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=398, Percent_Identity=32.4120603015075, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI303304987, Length=239, Percent_Identity=32.6359832635983, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI21269877, Length=239, Percent_Identity=32.6359832635983, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI10835051, Length=239, Percent_Identity=32.6359832635983, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI62240992, Length=239, Percent_Identity=32.6359832635983, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1786737, Length=402, Percent_Identity=36.318407960199, Blast_Score=242, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17509795, Length=262, Percent_Identity=31.2977099236641, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17509797, Length=262, Percent_Identity=31.2977099236641, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324082, Length=344, Percent_Identity=28.1976744186047, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922218, Length=423, Percent_Identity=31.6784869976359, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI19922364, Length=236, Percent_Identity=29.6610169491525, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_MYCSJ (A3Q1B7)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071435.1
- ProteinModelPortal:   A3Q1B7
- SMR:   A3Q1B7
- STRING:   A3Q1B7
- EnsemblBacteria:   EBMYCT00000050380
- GeneID:   4878878
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3165
- eggNOG:   COG0215
- GeneTree:   EBGT00050000016491
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- BioCyc:   MSP164757:MJLS_3165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.3.1.13

Molecular weight: Translated: 45517; Mature: 45517

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: BINDING 58-58 BINDING 227-227 BINDING 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESWSAPEVPALPGRGPQLRLYDSADRQVRPVSAGDTATMYVCGITPYDATHLGHAATYL
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
AFDLVHRLWLDAGHRVHYVQNITDVDDPLFERAARDGIDWRDLGAREIQLFREDMAALRV
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHEE
LPPHDYVAATDAIAEVIELVEKMLASGAAYVVDDPEFPDVYYRADATVQFGYESNYDHET
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCEEEECCCCCCCHHH
MLTLFAERGGDPDRAGKADELDALLWRAERPGEPSWPSPFGPGRPGWHVECAAIALSRIG
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
TGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARHYVHAGMIGWDGHKMSKSRGNLVLV
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEE
SRLRAEGVDPSAIRLGLLAGHYREDRFWSDDVLSDAQTRLQRWRRATSLPTGPDATDVLA
EEHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RVRTYLADDLDTPKALIALDAWCTEALDGGGSDVTAPKTVATAVDALLGVALPVE
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MESWSAPEVPALPGRGPQLRLYDSADRQVRPVSAGDTATMYVCGITPYDATHLGHAATYL
CCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
AFDLVHRLWLDAGHRVHYVQNITDVDDPLFERAARDGIDWRDLGAREIQLFREDMAALRV
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHEE
LPPHDYVAATDAIAEVIELVEKMLASGAAYVVDDPEFPDVYYRADATVQFGYESNYDHET
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCEEEECCCCCCCHHH
MLTLFAERGGDPDRAGKADELDALLWRAERPGEPSWPSPFGPGRPGWHVECAAIALSRIG
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
TGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARHYVHAGMIGWDGHKMSKSRGNLVLV
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEE
SRLRAEGVDPSAIRLGLLAGHYREDRFWSDDVLSDAQTRLQRWRRATSLPTGPDATDVLA
EEHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RVRTYLADDLDTPKALIALDAWCTEALDGGGSDVTAPKTVATAVDALLGVALPVE
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA