Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 108799985

GI number: 108799985

Start: 3190094

End: 3191131

Strand: Reverse

Name: braD [H]

Synonym: Mmcs_3019

Alternate gene names: 108799985

Gene position: 3191131-3190094 (Counterclockwise)

Preceding gene: 108799986

Following gene: 108799984

Centisome position: 55.93

GC content: 64.45

Gene sequence:

>1038_bases
ATGATCGCCGAGTGCGTCGACCAGTACGGCTGTCTGGCGGCGAATATCAGCTTCAACCTGGAGGGCCTGCGAAACGGCTT
CTGGCAGTTGACGATCGACGGATTGTCCTGGGGTGCCATCTATGCCCTGGTGGCGGTCGGTTACACGCTGGTGTTCGGCG
TGCTGCGGCTGATCAACTTCGCGCACTCCGAGATCTTCATGCTGGGGATGTTCGGCGCCTACTTCTGCCTCGACTTCATC
CTGGGATTCACCCCGTCCGGCAATGCCTACAACAAGGGCATCGCGCTGACGGTGCTGTACCTGGTGATCGCGATGCTGTT
CGCGATGCTGGTGTCCGGATCGGCGGCCGTCGGTCTGGAGGCGGTGGCCTACCGGCCGCTGCGAAGACGCGGGGCGCGGC
CGCTGACGTTCCTCATCACCGCCATCGGCATGTCGTTCGTGCTGCAGGAATTCGTCCACTTCATCCTGCCGAAGATCATC
AAGGGTTACGGCGGCAGCAACGCCCAGCAGCCGATCACGCTGGTGCAACCCAAGACCCAGTTCGAGATCTTCGGCGCGTC
GGTCTCCAACATCACGCTGGTCATCATCGGGGCGGCCCTGCTCTTCGCGCTGCTGACCGACATCGCCATCAACCGCACGA
AGTTCGGACGCGGCATCCGCGCGGTGGCGCAGGATCCGAACACCGCGACCCTGATGGGGGTGTCCCGGGAACGGGTGATC
ATGCTGACGTTCATGATCGGGGGTCTGCTGGCCGGGGCCGCGGCGCTGCTCTACACCCTCAAGGTGCCGCAGGGCATCAT
CTACTCCGGCGGTTTTCTGCTCGGTATCAAGGCGTTCTCCGCCGCGGTGCTCGGCGGCATCGGCAACCTGCGCGGTGCGC
TGCTCGGCGGGCTCATCCTGGGTGTCATGGAGAACTACGGGCAGGCGGTGTTCGGCACCCAGTGGCGTGACGTGGTCGCC
TTCGTCCTGCTGGTTCTGGTCCTGCTCTTCCGCCCCACCGGCATACTCGGTGAGAGCCTCGGGAAGGCGCGGGTATGA

Upstream 100 bases:

>100_bases
GTACAAGGTCCAGTGATCGACTAGGCGAGACGCGTCCGAACCAGTTGGCTCGGACGCGTTTCGTTTAGCACCCCCACCGC
GCGAGGAGCCCACCGCACCG

Downstream 100 bases:

>100_bases
GCGGTCAACGGGCGAACGTGTGGCAGGCGCTCGTCATGCGCGTGCGGGCCGTGTGGGCGGTCCTCGTCGAGCGCACGCGA
CCGGTGTGGGAACGGTTGCG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIAECVDQYGCLAANISFNLEGLRNGFWQLTIDGLSWGAIYALVAVGYTLVFGVLRLINFAHSEIFMLGMFGAYFCLDFI
LGFTPSGNAYNKGIALTVLYLVIAMLFAMLVSGSAAVGLEAVAYRPLRRRGARPLTFLITAIGMSFVLQEFVHFILPKII
KGYGGSNAQQPITLVQPKTQFEIFGASVSNITLVIIGAALLFALLTDIAINRTKFGRGIRAVAQDPNTATLMGVSRERVI
MLTFMIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKAFSAAVLGGIGNLRGALLGGLILGVMENYGQAVFGTQWRDVVA
FVLLVLVLLFRPTGILGESLGKARV

Sequences:

>Translated_345_residues
MIAECVDQYGCLAANISFNLEGLRNGFWQLTIDGLSWGAIYALVAVGYTLVFGVLRLINFAHSEIFMLGMFGAYFCLDFI
LGFTPSGNAYNKGIALTVLYLVIAMLFAMLVSGSAAVGLEAVAYRPLRRRGARPLTFLITAIGMSFVLQEFVHFILPKII
KGYGGSNAQQPITLVQPKTQFEIFGASVSNITLVIIGAALLFALLTDIAINRTKFGRGIRAVAQDPNTATLMGVSRERVI
MLTFMIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKAFSAAVLGGIGNLRGALLGGLILGVMENYGQAVFGTQWRDVVA
FVLLVLVLLFRPTGILGESLGKARV
>Mature_345_residues
MIAECVDQYGCLAANISFNLEGLRNGFWQLTIDGLSWGAIYALVAVGYTLVFGVLRLINFAHSEIFMLGMFGAYFCLDFI
LGFTPSGNAYNKGIALTVLYLVIAMLFAMLVSGSAAVGLEAVAYRPLRRRGARPLTFLITAIGMSFVLQEFVHFILPKII
KGYGGSNAQQPITLVQPKTQFEIFGASVSNITLVIIGAALLFALLTDIAINRTKFGRGIRAVAQDPNTATLMGVSRERVI
MLTFMIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKAFSAAVLGGIGNLRGALLGGLILGVMENYGQAVFGTQWRDVVA
FVLLVLVLLFRPTGILGESLGKARV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=320, Percent_Identity=37.8125, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 36864; Mature: 36864

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAECVDQYGCLAANISFNLEGLRNGFWQLTIDGLSWGAIYALVAVGYTLVFGVLRLINF
CHHHHHHHHCCEEEECEEEHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AHSEIFMLGMFGAYFCLDFILGFTPSGNAYNKGIALTVLYLVIAMLFAMLVSGSAAVGLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AVAYRPLRRRGARPLTFLITAIGMSFVLQEFVHFILPKIIKGYGGSNAQQPITLVQPKTQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
FEIFGASVSNITLVIIGAALLFALLTDIAINRTKFGRGIRAVAQDPNTATLMGVSRERVI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHH
MLTFMIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKAFSAAVLGGIGNLRGALLGGLIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMENYGQAVFGTQWRDVVAFVLLVLVLLFRPTGILGESLGKARV
HHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure
MIAECVDQYGCLAANISFNLEGLRNGFWQLTIDGLSWGAIYALVAVGYTLVFGVLRLINF
CHHHHHHHHCCEEEECEEEHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AHSEIFMLGMFGAYFCLDFILGFTPSGNAYNKGIALTVLYLVIAMLFAMLVSGSAAVGLE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AVAYRPLRRRGARPLTFLITAIGMSFVLQEFVHFILPKIIKGYGGSNAQQPITLVQPKTQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
FEIFGASVSNITLVIIGAALLFALLTDIAINRTKFGRGIRAVAQDPNTATLMGVSRERVI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHH
MLTFMIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKAFSAAVLGGIGNLRGALLGGLIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMENYGQAVFGTQWRDVVAFVLLVLVLLFRPTGILGESLGKARV
HHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]