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The map label for this gene is sigF [H]

Identifier: 108799863

GI number: 108799863

Start: 3059019

End: 3059828

Strand: Reverse

Name: sigF [H]

Synonym: Mmcs_2897

Alternate gene names: 108799863

Gene position: 3059828-3059019 (Counterclockwise)

Preceding gene: 108799865

Following gene: 108799861

Centisome position: 53.63

GC content: 64.69

Gene sequence:

>810_bases
ATGACGATCATTGACGACACACTCCAGGTGACGGCCAAAAGGCCGTCCACTCGTACCCAAGACGACTACACCGATGTCCC
GGACATGTTCCGCCTGCTGTCGGTATTGAGCCCCGGAACCCCGGATCACGACCGGCAGCGTGAACGCATCGTGACGCGAT
GCCTTCCGCTGGCCGACCACATCGCTTTCCGCTATCACCGCAAGGGCGAGAACCTCGACGATCTCGTGCAGATCGCCCGA
GTCGGGTTGATGCATGCGGTGAACCGCTTCGATCCCGAGAACGGCGCCAACTTCCTGTCGTTCGCAGTGCCCACGATGCT
CGGCGAGATCCGACGCCACTTCCGGGACAACGGATGGGCGATGCACGTCCCACGCCGGCTCAAGGATCTGCACGTGCAGA
TCACCCGGGCGACCCCGCGGCTGACGCAGTCGCTCGGCCGTCCCCCCACACCCACCGATCTGGCCGCCGAACTCGGGGCC
GACCGGCAGGAAGTGGTGGAGTGCCTGGTTGCCGGTGACGCGTACAGCCTGCGCTCGATCGACAGCCCCATCTCATCGTC
GGACGACACGACGCGGTCGCTCGCCGACACGCTCGGCGAGGTCGACGGGGAGATGGAGCACATCACCGACCGCGAGTCGC
TGCGTCCGCTGCTGGCGGCGCTGACCGAACGTGAGCGCACTGTGATCAGTCTCAGGTTCTTCGCCTCGATGACGCAGACG
CAGATCGCCGAGCGAATCGGTGTCTCGCAGATGCAGGTCTCGCGAATTCTCGCCAAGACACTTCAGCAGTTGCGCGACGG
GATGAGCTGA

Upstream 100 bases:

>100_bases
TGGCTCCCGTTGGCCACAGCACCGTCTCCCGGATAAGCAAGGCATGCGTCGAACAGCCGGCGTCGCCTTCGCGTCCGCAC
CGAGTCCGGAAGGACCCGCG

Downstream 100 bases:

>100_bases
CCCTGTAGGTCAGCCCCCGGAGCGCTGCCGGACCGCGACCGCGATCTCGTTGCGACGCCGGAACGGCAGCGTCCACGGCG
GATCGTAGAACCATGCCGCC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTIIDDTLQVTAKRPSTRTQDDYTDVPDMFRLLSVLSPGTPDHDRQRERIVTRCLPLADHIAFRYHRKGENLDDLVQIAR
VGLMHAVNRFDPENGANFLSFAVPTMLGEIRRHFRDNGWAMHVPRRLKDLHVQITRATPRLTQSLGRPPTPTDLAAELGA
DRQEVVECLVAGDAYSLRSIDSPISSSDDTTRSLADTLGEVDGEMEHITDRESLRPLLAALTERERTVISLRFFASMTQT
QIAERIGVSQMQVSRILAKTLQQLRDGMS

Sequences:

>Translated_269_residues
MTIIDDTLQVTAKRPSTRTQDDYTDVPDMFRLLSVLSPGTPDHDRQRERIVTRCLPLADHIAFRYHRKGENLDDLVQIAR
VGLMHAVNRFDPENGANFLSFAVPTMLGEIRRHFRDNGWAMHVPRRLKDLHVQITRATPRLTQSLGRPPTPTDLAAELGA
DRQEVVECLVAGDAYSLRSIDSPISSSDDTTRSLADTLGEVDGEMEHITDRESLRPLLAALTERERTVISLRFFASMTQT
QIAERIGVSQMQVSRILAKTLQQLRDGMS
>Mature_268_residues
TIIDDTLQVTAKRPSTRTQDDYTDVPDMFRLLSVLSPGTPDHDRQRERIVTRCLPLADHIAFRYHRKGENLDDLVQIARV
GLMHAVNRFDPENGANFLSFAVPTMLGEIRRHFRDNGWAMHVPRRLKDLHVQITRATPRLTQSLGRPPTPTDLAAELGAD
RQEVVECLVAGDAYSLRSIDSPISSSDDTTRSLADTLGEVDGEMEHITDRESLRPLLAALTERERTVISLRFFASMTQTQ
IAERIGVSQMQVSRILAKTLQQLRDGMS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=28.4, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788231, Length=219, Percent_Identity=26.9406392694064, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789098, Length=230, Percent_Identity=26.9565217391304, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30327; Mature: 30196

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIDDTLQVTAKRPSTRTQDDYTDVPDMFRLLSVLSPGTPDHDRQRERIVTRCLPLADH
CCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IAFRYHRKGENLDDLVQIARVGLMHAVNRFDPENGANFLSFAVPTMLGEIRRHFRDNGWA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
MHVPRRLKDLHVQITRATPRLTQSLGRPPTPTDLAAELGADRQEVVECLVAGDAYSLRSI
EECHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHC
DSPISSSDDTTRSLADTLGEVDGEMEHITDRESLRPLLAALTERERTVISLRFFASMTQT
CCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIAERIGVSQMQVSRILAKTLQQLRDGMS
HHHHHHCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIIDDTLQVTAKRPSTRTQDDYTDVPDMFRLLSVLSPGTPDHDRQRERIVTRCLPLADH
CCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
IAFRYHRKGENLDDLVQIARVGLMHAVNRFDPENGANFLSFAVPTMLGEIRRHFRDNGWA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
MHVPRRLKDLHVQITRATPRLTQSLGRPPTPTDLAAELGADRQEVVECLVAGDAYSLRSI
EECHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHC
DSPISSSDDTTRSLADTLGEVDGEMEHITDRESLRPLLAALTERERTVISLRFFASMTQT
CCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIAERIGVSQMQVSRILAKTLQQLRDGMS
HHHHHHCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]